Rapid testing requires clinical evaluation for accurate diagnosis of dengue disease: A passive surveillance study in Southern Malaysia.
Background Dengue fever is the most common mosquito-borne infection worldwide where an expanding surveillance and characterization of this infection are needed to better inform the healthcare system. In this surveillance-based study, we explored the prevalence and distinguishing features of dengue f...
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ftdoajarticles:oai:doaj.org/article:d9382a3e44c449b08d2966163688c7f6 2023-05-15T15:17:43+02:00 Rapid testing requires clinical evaluation for accurate diagnosis of dengue disease: A passive surveillance study in Southern Malaysia. Chin Fang Ngim Syed M Tupur Husain Sharifah Syed Hassan Amreeta Dhanoa Siti Aisyah Abdul Ahmad Jeevitha Mariapun Wan Fadhilah Wan Ismail Nevein Philip Botross Henien Nowrozy Kamar Jahan Lian Yih Pong Hesham Elshahawi Robert D Hontz Tyler Warkentein Nor'azim Mohd Yunos 2021-05-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0009445 https://doaj.org/article/d9382a3e44c449b08d2966163688c7f6 EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pntd.0009445 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0009445 https://doaj.org/article/d9382a3e44c449b08d2966163688c7f6 PLoS Neglected Tropical Diseases, Vol 15, Iss 5, p e0009445 (2021) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2021 ftdoajarticles https://doi.org/10.1371/journal.pntd.0009445 2022-12-31T08:00:27Z Background Dengue fever is the most common mosquito-borne infection worldwide where an expanding surveillance and characterization of this infection are needed to better inform the healthcare system. In this surveillance-based study, we explored the prevalence and distinguishing features of dengue fever amongst febrile patients in a large community-based health facility in southern peninsular Malaysia. Methods Over six months in 2018, we recruited 368 adults who met the WHO 2009 criteria for probable dengue infection. They underwent the following blood tests: full blood count, dengue virus (DENV) rapid diagnostic test (RDT), ELISA (dengue IgM and IgG), nested RT-PCR for dengue, multiplex qRT-PCR for Zika, Chikungunya and dengue as well as PCR tests for Leptopspira spp., Japanese encephalitis and West Nile virus. Results Laboratory-confirmed dengue infections (defined by positive tests in NS1, IgM, high-titre IgG or nested RT-PCR) were found in 167 (45.4%) patients. Of these 167 dengue patients, only 104 (62.3%) were positive on rapid diagnostic testing. Dengue infection was significantly associated with the following features: family or neighbours with dengue in the past week (AOR: 3.59, 95% CI:2.14-6.00, p<0.001), cutaneous rash (AOR: 3.58, 95% CI:1.77-7.23, p<0.001), increased temperature (AOR: 1.33, 95% CI:1.04-1.70, p = 0.021), leucopenia (white cell count < 4,000/μL) (AOR: 3.44, 95% CI:1.72-6.89, p<0.001) and thrombocytopenia (platelet count <150,000/μL)(AOR: 4.63, 95% CI:2.33-9.21, p<0.001). Dengue infection was negatively associated with runny nose (AOR: 0.47, 95% CI:0.29-0.78, p = 0.003) and arthralgia (AOR: 0.42, 95% CI:0.24-0.75, p = 0.004). Serotyping by nested RT-PCR revealed mostly mono-infections with DENV-2 (n = 64), DENV-1 (n = 32) and DENV-3 (n = 17); 14 co-infections occurred with DENV-1/DENV-2 (n = 13) and DENV-1/DENV-4 (n = 1). Besides dengue, none of the pathogens above were found in patients' serum. Conclusions Acute undifferentiated febrile infections are a diagnostic ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 15 5 e0009445 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Chin Fang Ngim Syed M Tupur Husain Sharifah Syed Hassan Amreeta Dhanoa Siti Aisyah Abdul Ahmad Jeevitha Mariapun Wan Fadhilah Wan Ismail Nevein Philip Botross Henien Nowrozy Kamar Jahan Lian Yih Pong Hesham Elshahawi Robert D Hontz Tyler Warkentein Nor'azim Mohd Yunos Rapid testing requires clinical evaluation for accurate diagnosis of dengue disease: A passive surveillance study in Southern Malaysia. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Background Dengue fever is the most common mosquito-borne infection worldwide where an expanding surveillance and characterization of this infection are needed to better inform the healthcare system. In this surveillance-based study, we explored the prevalence and distinguishing features of dengue fever amongst febrile patients in a large community-based health facility in southern peninsular Malaysia. Methods Over six months in 2018, we recruited 368 adults who met the WHO 2009 criteria for probable dengue infection. They underwent the following blood tests: full blood count, dengue virus (DENV) rapid diagnostic test (RDT), ELISA (dengue IgM and IgG), nested RT-PCR for dengue, multiplex qRT-PCR for Zika, Chikungunya and dengue as well as PCR tests for Leptopspira spp., Japanese encephalitis and West Nile virus. Results Laboratory-confirmed dengue infections (defined by positive tests in NS1, IgM, high-titre IgG or nested RT-PCR) were found in 167 (45.4%) patients. Of these 167 dengue patients, only 104 (62.3%) were positive on rapid diagnostic testing. Dengue infection was significantly associated with the following features: family or neighbours with dengue in the past week (AOR: 3.59, 95% CI:2.14-6.00, p<0.001), cutaneous rash (AOR: 3.58, 95% CI:1.77-7.23, p<0.001), increased temperature (AOR: 1.33, 95% CI:1.04-1.70, p = 0.021), leucopenia (white cell count < 4,000/μL) (AOR: 3.44, 95% CI:1.72-6.89, p<0.001) and thrombocytopenia (platelet count <150,000/μL)(AOR: 4.63, 95% CI:2.33-9.21, p<0.001). Dengue infection was negatively associated with runny nose (AOR: 0.47, 95% CI:0.29-0.78, p = 0.003) and arthralgia (AOR: 0.42, 95% CI:0.24-0.75, p = 0.004). Serotyping by nested RT-PCR revealed mostly mono-infections with DENV-2 (n = 64), DENV-1 (n = 32) and DENV-3 (n = 17); 14 co-infections occurred with DENV-1/DENV-2 (n = 13) and DENV-1/DENV-4 (n = 1). Besides dengue, none of the pathogens above were found in patients' serum. Conclusions Acute undifferentiated febrile infections are a diagnostic ... |
format |
Article in Journal/Newspaper |
author |
Chin Fang Ngim Syed M Tupur Husain Sharifah Syed Hassan Amreeta Dhanoa Siti Aisyah Abdul Ahmad Jeevitha Mariapun Wan Fadhilah Wan Ismail Nevein Philip Botross Henien Nowrozy Kamar Jahan Lian Yih Pong Hesham Elshahawi Robert D Hontz Tyler Warkentein Nor'azim Mohd Yunos |
author_facet |
Chin Fang Ngim Syed M Tupur Husain Sharifah Syed Hassan Amreeta Dhanoa Siti Aisyah Abdul Ahmad Jeevitha Mariapun Wan Fadhilah Wan Ismail Nevein Philip Botross Henien Nowrozy Kamar Jahan Lian Yih Pong Hesham Elshahawi Robert D Hontz Tyler Warkentein Nor'azim Mohd Yunos |
author_sort |
Chin Fang Ngim |
title |
Rapid testing requires clinical evaluation for accurate diagnosis of dengue disease: A passive surveillance study in Southern Malaysia. |
title_short |
Rapid testing requires clinical evaluation for accurate diagnosis of dengue disease: A passive surveillance study in Southern Malaysia. |
title_full |
Rapid testing requires clinical evaluation for accurate diagnosis of dengue disease: A passive surveillance study in Southern Malaysia. |
title_fullStr |
Rapid testing requires clinical evaluation for accurate diagnosis of dengue disease: A passive surveillance study in Southern Malaysia. |
title_full_unstemmed |
Rapid testing requires clinical evaluation for accurate diagnosis of dengue disease: A passive surveillance study in Southern Malaysia. |
title_sort |
rapid testing requires clinical evaluation for accurate diagnosis of dengue disease: a passive surveillance study in southern malaysia. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2021 |
url |
https://doi.org/10.1371/journal.pntd.0009445 https://doaj.org/article/d9382a3e44c449b08d2966163688c7f6 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 15, Iss 5, p e0009445 (2021) |
op_relation |
https://doi.org/10.1371/journal.pntd.0009445 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0009445 https://doaj.org/article/d9382a3e44c449b08d2966163688c7f6 |
op_doi |
https://doi.org/10.1371/journal.pntd.0009445 |
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PLOS Neglected Tropical Diseases |
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15 |
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5 |
container_start_page |
e0009445 |
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