Species Delimitation of Hexacorallia and Octocorallia Around Iceland Using Nuclear and Mitochondrial DNA and Proteome Fingerprinting
Cold-water corals build up reef structures or coral gardens and play an important role for many organisms in the deep sea. Climate change, deep-sea mining, and bottom trawling are severely compromising these ecosystems, making it all the more important to document the diversity, distribution, and im...
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ftdoajarticles:oai:doaj.org/article:d927db5c7bb046a59f9dfa1107187e23 2023-05-15T16:51:25+02:00 Species Delimitation of Hexacorallia and Octocorallia Around Iceland Using Nuclear and Mitochondrial DNA and Proteome Fingerprinting Severin A. Korfhage Sven Rossel Saskia Brix Catherine S. McFadden Steinunn Hilma Ólafsdóttir Pedro Martínez Arbizu 2022-03-01T00:00:00Z https://doi.org/10.3389/fmars.2022.838201 https://doaj.org/article/d927db5c7bb046a59f9dfa1107187e23 EN eng Frontiers Media S.A. https://www.frontiersin.org/articles/10.3389/fmars.2022.838201/full https://doaj.org/toc/2296-7745 2296-7745 doi:10.3389/fmars.2022.838201 https://doaj.org/article/d927db5c7bb046a59f9dfa1107187e23 Frontiers in Marine Science, Vol 9 (2022) COI 28S rDNA mtMutS GMYC MALDI-TOF MS Anthozoa Science Q General. Including nature conservation geographical distribution QH1-199.5 article 2022 ftdoajarticles https://doi.org/10.3389/fmars.2022.838201 2022-12-31T16:12:34Z Cold-water corals build up reef structures or coral gardens and play an important role for many organisms in the deep sea. Climate change, deep-sea mining, and bottom trawling are severely compromising these ecosystems, making it all the more important to document the diversity, distribution, and impacts on corals. This goes hand in hand with species identification, which is morphologically and genetically challenging for Hexa- and Octocorallia. Morphological variation and slowly evolving molecular markers both contribute to the difficulty of species identification. In this study, a fast and cheap species delimitation tool for Octocorallia and Scleractinia, an order of the Hexacorallia, of the Northeast Atlantic was tested based on 49 specimens. Two nuclear markers (ITS2 and 28S rDNA) and two mitochondrial markers (COI and mtMutS) were sequenced. The sequences formed the basis of a reference library for comparison to the results of species delimitation based on proteomic fingerprinting using MALDI-TOF MS. The genetic methods were able to distinguish 17 of 18 presumed species. Due to a lack of replicates, using proteome fingerprinting only 7 species were distinguishable. Species that could not be distinguished from one another still achieved good signals of spectra but were not represented by enough specimens for comparison. Therefore, it is predicted that with an extensive reference library of proteome spectra for Scleractinia and Octocorallia, MALDI-TOF MS may provide a rapid and cost-effective alternative for species discrimination in corals. Article in Journal/Newspaper Iceland Northeast Atlantic Directory of Open Access Journals: DOAJ Articles Frontiers in Marine Science 9 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
COI 28S rDNA mtMutS GMYC MALDI-TOF MS Anthozoa Science Q General. Including nature conservation geographical distribution QH1-199.5 |
spellingShingle |
COI 28S rDNA mtMutS GMYC MALDI-TOF MS Anthozoa Science Q General. Including nature conservation geographical distribution QH1-199.5 Severin A. Korfhage Sven Rossel Saskia Brix Catherine S. McFadden Steinunn Hilma Ólafsdóttir Pedro Martínez Arbizu Species Delimitation of Hexacorallia and Octocorallia Around Iceland Using Nuclear and Mitochondrial DNA and Proteome Fingerprinting |
topic_facet |
COI 28S rDNA mtMutS GMYC MALDI-TOF MS Anthozoa Science Q General. Including nature conservation geographical distribution QH1-199.5 |
description |
Cold-water corals build up reef structures or coral gardens and play an important role for many organisms in the deep sea. Climate change, deep-sea mining, and bottom trawling are severely compromising these ecosystems, making it all the more important to document the diversity, distribution, and impacts on corals. This goes hand in hand with species identification, which is morphologically and genetically challenging for Hexa- and Octocorallia. Morphological variation and slowly evolving molecular markers both contribute to the difficulty of species identification. In this study, a fast and cheap species delimitation tool for Octocorallia and Scleractinia, an order of the Hexacorallia, of the Northeast Atlantic was tested based on 49 specimens. Two nuclear markers (ITS2 and 28S rDNA) and two mitochondrial markers (COI and mtMutS) were sequenced. The sequences formed the basis of a reference library for comparison to the results of species delimitation based on proteomic fingerprinting using MALDI-TOF MS. The genetic methods were able to distinguish 17 of 18 presumed species. Due to a lack of replicates, using proteome fingerprinting only 7 species were distinguishable. Species that could not be distinguished from one another still achieved good signals of spectra but were not represented by enough specimens for comparison. Therefore, it is predicted that with an extensive reference library of proteome spectra for Scleractinia and Octocorallia, MALDI-TOF MS may provide a rapid and cost-effective alternative for species discrimination in corals. |
format |
Article in Journal/Newspaper |
author |
Severin A. Korfhage Sven Rossel Saskia Brix Catherine S. McFadden Steinunn Hilma Ólafsdóttir Pedro Martínez Arbizu |
author_facet |
Severin A. Korfhage Sven Rossel Saskia Brix Catherine S. McFadden Steinunn Hilma Ólafsdóttir Pedro Martínez Arbizu |
author_sort |
Severin A. Korfhage |
title |
Species Delimitation of Hexacorallia and Octocorallia Around Iceland Using Nuclear and Mitochondrial DNA and Proteome Fingerprinting |
title_short |
Species Delimitation of Hexacorallia and Octocorallia Around Iceland Using Nuclear and Mitochondrial DNA and Proteome Fingerprinting |
title_full |
Species Delimitation of Hexacorallia and Octocorallia Around Iceland Using Nuclear and Mitochondrial DNA and Proteome Fingerprinting |
title_fullStr |
Species Delimitation of Hexacorallia and Octocorallia Around Iceland Using Nuclear and Mitochondrial DNA and Proteome Fingerprinting |
title_full_unstemmed |
Species Delimitation of Hexacorallia and Octocorallia Around Iceland Using Nuclear and Mitochondrial DNA and Proteome Fingerprinting |
title_sort |
species delimitation of hexacorallia and octocorallia around iceland using nuclear and mitochondrial dna and proteome fingerprinting |
publisher |
Frontiers Media S.A. |
publishDate |
2022 |
url |
https://doi.org/10.3389/fmars.2022.838201 https://doaj.org/article/d927db5c7bb046a59f9dfa1107187e23 |
genre |
Iceland Northeast Atlantic |
genre_facet |
Iceland Northeast Atlantic |
op_source |
Frontiers in Marine Science, Vol 9 (2022) |
op_relation |
https://www.frontiersin.org/articles/10.3389/fmars.2022.838201/full https://doaj.org/toc/2296-7745 2296-7745 doi:10.3389/fmars.2022.838201 https://doaj.org/article/d927db5c7bb046a59f9dfa1107187e23 |
op_doi |
https://doi.org/10.3389/fmars.2022.838201 |
container_title |
Frontiers in Marine Science |
container_volume |
9 |
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1766041528175689728 |