Toxic thermoresistant metabolites of Fusarium oxysporum are capable of inducing histopathological alterations in Wistar rats
The genus Fusarium is known to produce mycotoxins that cause fusariosis in plants, animals and humans. Mycotoxins are among the virulence factors of this genus. Metabolic extracts of Fusarium oxysporum, isolated from a patient with onychomycosis and sterilized by filtration or autoclave, were inocul...
Published in: | Journal of Venomous Animals and Toxins including Tropical Diseases |
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ftdoajarticles:oai:doaj.org/article:d91293c2e8c3468a83023d50d83cea05 2023-05-15T15:09:39+02:00 Toxic thermoresistant metabolites of Fusarium oxysporum are capable of inducing histopathological alterations in Wistar rats L Hernandes AV Marangon T Salci TIE Svidzinski 2012-01-01T00:00:00Z https://doi.org/10.1590/S1678-91992012000200003 https://doaj.org/article/d91293c2e8c3468a83023d50d83cea05 EN eng SciELO http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992012000200003 https://doaj.org/toc/1678-9199 doi:10.1590/S1678-91992012000200003 1678-9199 https://doaj.org/article/d91293c2e8c3468a83023d50d83cea05 Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 18, Iss 2, Pp 144-149 (2012) fungal skin disease Fusarium mycotoxins Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 article 2012 ftdoajarticles https://doi.org/10.1590/S1678-91992012000200003 2022-12-31T01:54:28Z The genus Fusarium is known to produce mycotoxins that cause fusariosis in plants, animals and humans. Mycotoxins are among the virulence factors of this genus. Metabolic extracts of Fusarium oxysporum, isolated from a patient with onychomycosis and sterilized by filtration or autoclave, were inoculated intradermally into Wistar rats at concentrations of 0.25, 0.5 and 1 µg/µL, and the effects on their tegument were observed at 24 and 72 hours. After histological procedures and staining by hematoxylineosin, the sections were studied for their inflammatory-reaction intensity and for evidence of injury and tissue distortion. Inflammatory reactions in the dermis and the subcutaneous tissue were observed at all concentrations of the inoculated extract tested. There was a significant influx of neutrophils, mastocytes and lymphocytes, as well as a large quantity of macrophages. Apoptotic bodies and hyperemic blood vessels were observed. This reaction was directly related to the extract concentration, and was most intense in animals that received the 1 mg/µL dose. The maximum peak was observed at 24 hours. The autoclaved metabolic extract produced the same effects as the untreated one, indicating the presence of heat-resistant metabolites. In conclusion, the metabolic extracts obtained from sterilized culture filtrates of F. oxysporum are capable of inducing an inflammatory response within 24 hours in the dermis and subcutaneous tissue of rats. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Journal of Venomous Animals and Toxins including Tropical Diseases 18 2 144 149 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
fungal skin disease Fusarium mycotoxins Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
spellingShingle |
fungal skin disease Fusarium mycotoxins Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 L Hernandes AV Marangon T Salci TIE Svidzinski Toxic thermoresistant metabolites of Fusarium oxysporum are capable of inducing histopathological alterations in Wistar rats |
topic_facet |
fungal skin disease Fusarium mycotoxins Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
description |
The genus Fusarium is known to produce mycotoxins that cause fusariosis in plants, animals and humans. Mycotoxins are among the virulence factors of this genus. Metabolic extracts of Fusarium oxysporum, isolated from a patient with onychomycosis and sterilized by filtration or autoclave, were inoculated intradermally into Wistar rats at concentrations of 0.25, 0.5 and 1 µg/µL, and the effects on their tegument were observed at 24 and 72 hours. After histological procedures and staining by hematoxylineosin, the sections were studied for their inflammatory-reaction intensity and for evidence of injury and tissue distortion. Inflammatory reactions in the dermis and the subcutaneous tissue were observed at all concentrations of the inoculated extract tested. There was a significant influx of neutrophils, mastocytes and lymphocytes, as well as a large quantity of macrophages. Apoptotic bodies and hyperemic blood vessels were observed. This reaction was directly related to the extract concentration, and was most intense in animals that received the 1 mg/µL dose. The maximum peak was observed at 24 hours. The autoclaved metabolic extract produced the same effects as the untreated one, indicating the presence of heat-resistant metabolites. In conclusion, the metabolic extracts obtained from sterilized culture filtrates of F. oxysporum are capable of inducing an inflammatory response within 24 hours in the dermis and subcutaneous tissue of rats. |
format |
Article in Journal/Newspaper |
author |
L Hernandes AV Marangon T Salci TIE Svidzinski |
author_facet |
L Hernandes AV Marangon T Salci TIE Svidzinski |
author_sort |
L Hernandes |
title |
Toxic thermoresistant metabolites of Fusarium oxysporum are capable of inducing histopathological alterations in Wistar rats |
title_short |
Toxic thermoresistant metabolites of Fusarium oxysporum are capable of inducing histopathological alterations in Wistar rats |
title_full |
Toxic thermoresistant metabolites of Fusarium oxysporum are capable of inducing histopathological alterations in Wistar rats |
title_fullStr |
Toxic thermoresistant metabolites of Fusarium oxysporum are capable of inducing histopathological alterations in Wistar rats |
title_full_unstemmed |
Toxic thermoresistant metabolites of Fusarium oxysporum are capable of inducing histopathological alterations in Wistar rats |
title_sort |
toxic thermoresistant metabolites of fusarium oxysporum are capable of inducing histopathological alterations in wistar rats |
publisher |
SciELO |
publishDate |
2012 |
url |
https://doi.org/10.1590/S1678-91992012000200003 https://doaj.org/article/d91293c2e8c3468a83023d50d83cea05 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 18, Iss 2, Pp 144-149 (2012) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992012000200003 https://doaj.org/toc/1678-9199 doi:10.1590/S1678-91992012000200003 1678-9199 https://doaj.org/article/d91293c2e8c3468a83023d50d83cea05 |
op_doi |
https://doi.org/10.1590/S1678-91992012000200003 |
container_title |
Journal of Venomous Animals and Toxins including Tropical Diseases |
container_volume |
18 |
container_issue |
2 |
container_start_page |
144 |
op_container_end_page |
149 |
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