In vitro detection of hepatitis C virus in platelets from uninfected individuals exposed to the virus
Introduction Despite hepatocytes being the target cells of hepatitis C virus (HCV), viral ribonucleic acid RNA has been detected in other cells, including platelets, which have been described as carriers of the virus in the circulation of infected patients. Platelets do not express cluster different...
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ftdoajarticles:oai:doaj.org/article:d8ed078fa2f842cf8395a2d85fa4fe38 2023-05-15T15:08:52+02:00 In vitro detection of hepatitis C virus in platelets from uninfected individuals exposed to the virus Juliana Lara Padovani Silvia Maria Corvino Jan Felix Drexler Giovanni Faria Silva Maria Inês de Moura Campos Pardini Rejane Maria Tommasini Grotto 2013-02-01T00:00:00Z https://doaj.org/article/d8ed078fa2f842cf8395a2d85fa4fe38 EN eng Sociedade Brasileira de Medicina Tropical (SBMT) http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822013000200154 https://doaj.org/toc/0037-8682 https://doaj.org/toc/1678-9849 0037-8682 1678-9849 https://doaj.org/article/d8ed078fa2f842cf8395a2d85fa4fe38 Revista da Sociedade Brasileira de Medicina Tropical, Vol 46, Iss 2, Pp 154-155 (2013) HCV Platelets CD81 Arctic medicine. Tropical medicine RC955-962 article 2013 ftdoajarticles 2022-12-30T23:08:11Z Introduction Despite hepatocytes being the target cells of hepatitis C virus (HCV), viral ribonucleic acid RNA has been detected in other cells, including platelets, which have been described as carriers of the virus in the circulation of infected patients. Platelets do not express cluster differentiation 81 CD81, the main receptor for the virus in hepatocytes, although this receptor protein has been found in megakaryocytes. Still, it is not clear if HCV interacts with platelets directly or if this interaction is a consequence of its association with megakaryocytes. The aim of this study was to evaluate the interaction of HCV with platelets from non-infected individuals, after in vitro exposure to the virus. Methods Platelets obtained from 50 blood donors not infected by HCV were incubated in vitro at 37°C for 48h with serum containing 100,000IU∕mL of genotype 1 HCV. After incubation, RNA extracted from the platelets was assayed for the presence of HCV by reverse transcription – polymerase chain reaction RT-PCR. Results After incubation in the presence of virus, all samples of platelets showed HCV RNA. Conclusions The results demonstrate that, in vitro, the virus interacts with platelets despite the absence of the receptor CD81, suggesting that other molecules could be involved in this association. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic |
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Directory of Open Access Journals: DOAJ Articles |
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English |
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HCV Platelets CD81 Arctic medicine. Tropical medicine RC955-962 |
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HCV Platelets CD81 Arctic medicine. Tropical medicine RC955-962 Juliana Lara Padovani Silvia Maria Corvino Jan Felix Drexler Giovanni Faria Silva Maria Inês de Moura Campos Pardini Rejane Maria Tommasini Grotto In vitro detection of hepatitis C virus in platelets from uninfected individuals exposed to the virus |
topic_facet |
HCV Platelets CD81 Arctic medicine. Tropical medicine RC955-962 |
description |
Introduction Despite hepatocytes being the target cells of hepatitis C virus (HCV), viral ribonucleic acid RNA has been detected in other cells, including platelets, which have been described as carriers of the virus in the circulation of infected patients. Platelets do not express cluster differentiation 81 CD81, the main receptor for the virus in hepatocytes, although this receptor protein has been found in megakaryocytes. Still, it is not clear if HCV interacts with platelets directly or if this interaction is a consequence of its association with megakaryocytes. The aim of this study was to evaluate the interaction of HCV with platelets from non-infected individuals, after in vitro exposure to the virus. Methods Platelets obtained from 50 blood donors not infected by HCV were incubated in vitro at 37°C for 48h with serum containing 100,000IU∕mL of genotype 1 HCV. After incubation, RNA extracted from the platelets was assayed for the presence of HCV by reverse transcription – polymerase chain reaction RT-PCR. Results After incubation in the presence of virus, all samples of platelets showed HCV RNA. Conclusions The results demonstrate that, in vitro, the virus interacts with platelets despite the absence of the receptor CD81, suggesting that other molecules could be involved in this association. |
format |
Article in Journal/Newspaper |
author |
Juliana Lara Padovani Silvia Maria Corvino Jan Felix Drexler Giovanni Faria Silva Maria Inês de Moura Campos Pardini Rejane Maria Tommasini Grotto |
author_facet |
Juliana Lara Padovani Silvia Maria Corvino Jan Felix Drexler Giovanni Faria Silva Maria Inês de Moura Campos Pardini Rejane Maria Tommasini Grotto |
author_sort |
Juliana Lara Padovani |
title |
In vitro detection of hepatitis C virus in platelets from uninfected individuals exposed to the virus |
title_short |
In vitro detection of hepatitis C virus in platelets from uninfected individuals exposed to the virus |
title_full |
In vitro detection of hepatitis C virus in platelets from uninfected individuals exposed to the virus |
title_fullStr |
In vitro detection of hepatitis C virus in platelets from uninfected individuals exposed to the virus |
title_full_unstemmed |
In vitro detection of hepatitis C virus in platelets from uninfected individuals exposed to the virus |
title_sort |
in vitro detection of hepatitis c virus in platelets from uninfected individuals exposed to the virus |
publisher |
Sociedade Brasileira de Medicina Tropical (SBMT) |
publishDate |
2013 |
url |
https://doaj.org/article/d8ed078fa2f842cf8395a2d85fa4fe38 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Revista da Sociedade Brasileira de Medicina Tropical, Vol 46, Iss 2, Pp 154-155 (2013) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822013000200154 https://doaj.org/toc/0037-8682 https://doaj.org/toc/1678-9849 0037-8682 1678-9849 https://doaj.org/article/d8ed078fa2f842cf8395a2d85fa4fe38 |
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1766340146559451136 |