Characterization and Cytotoxicity Studies of the Rare 21:4 n-7 Acid and Other Polyunsaturated Fatty Acids from the Marine Opisthobranch Scaphander lignarius, Isolated Using Bioassay Guided Fractionation
The marine opisthobranch Scaphander lignarius has been analyzed in the systematic search for novel bioactive compounds in Arctic marine organisms using bioassay guided fractionation. A number of highly cytotoxic fractions were shown to contain mainly polyunsaturated fatty acids (PUFAs). Selected PUF...
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2012
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ftdoajarticles:oai:doaj.org/article:d8d93548c8354bf68b291738420b7ab0 2023-05-15T15:07:50+02:00 Characterization and Cytotoxicity Studies of the Rare 21:4 n-7 Acid and Other Polyunsaturated Fatty Acids from the Marine Opisthobranch Scaphander lignarius, Isolated Using Bioassay Guided Fractionation Espen Hansen Johan Svenson Jeanette H. Andersen Terje Vasskog 2012-11-01T00:00:00Z https://doi.org/10.3390/md10122676 https://doaj.org/article/d8d93548c8354bf68b291738420b7ab0 EN eng MDPI AG http://www.mdpi.com/1660-3397/10/12/2676 https://doaj.org/toc/1660-3397 doi:10.3390/md10122676 1660-3397 https://doaj.org/article/d8d93548c8354bf68b291738420b7ab0 Marine Drugs, Vol 10, Iss 12, Pp 2676-2690 (2012) opisthobranch cytotoxic PUFA Scaphander lignarius ω 7 fatty acid Biology (General) QH301-705.5 article 2012 ftdoajarticles https://doi.org/10.3390/md10122676 2022-12-30T22:39:42Z The marine opisthobranch Scaphander lignarius has been analyzed in the systematic search for novel bioactive compounds in Arctic marine organisms using bioassay guided fractionation. A number of highly cytotoxic fractions were shown to contain mainly polyunsaturated fatty acids (PUFAs). Selected PUFAs were isolated and identified using both liquid chromatography-mass spectrometry (LC-MS) and nuclear magnetic resonance (NMR). It was shown that the opisthobranch contained unusual PUFAs such as several ω3 fatty acids and the ω7 heneicosa-5,8,11,14-tetraenoic acid (21:4 n-7) not isolated before. The organism was shown to be a very rich source of PUFAs and the activity of the isolated compounds against a range of human cancer cell lines (melanoma, colon carcinoma and breast carcinoma) is further reported. The ω7 PUFA was significantly more cytotoxic in comparison with reference ω6 arachidonic and ω3 eicosapentaenoic acid. A noteworthy non-selective cytotoxicity against normal lung fibroblasts was also established. The paper contains isolation protocols in addition to cytotoxicity data of the isolated compounds. The potential of marine mollusks as a source for rare PUFAs is also discussed. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Marine Drugs 10 12 2676 2690 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
opisthobranch cytotoxic PUFA Scaphander lignarius ω 7 fatty acid Biology (General) QH301-705.5 |
spellingShingle |
opisthobranch cytotoxic PUFA Scaphander lignarius ω 7 fatty acid Biology (General) QH301-705.5 Espen Hansen Johan Svenson Jeanette H. Andersen Terje Vasskog Characterization and Cytotoxicity Studies of the Rare 21:4 n-7 Acid and Other Polyunsaturated Fatty Acids from the Marine Opisthobranch Scaphander lignarius, Isolated Using Bioassay Guided Fractionation |
topic_facet |
opisthobranch cytotoxic PUFA Scaphander lignarius ω 7 fatty acid Biology (General) QH301-705.5 |
description |
The marine opisthobranch Scaphander lignarius has been analyzed in the systematic search for novel bioactive compounds in Arctic marine organisms using bioassay guided fractionation. A number of highly cytotoxic fractions were shown to contain mainly polyunsaturated fatty acids (PUFAs). Selected PUFAs were isolated and identified using both liquid chromatography-mass spectrometry (LC-MS) and nuclear magnetic resonance (NMR). It was shown that the opisthobranch contained unusual PUFAs such as several ω3 fatty acids and the ω7 heneicosa-5,8,11,14-tetraenoic acid (21:4 n-7) not isolated before. The organism was shown to be a very rich source of PUFAs and the activity of the isolated compounds against a range of human cancer cell lines (melanoma, colon carcinoma and breast carcinoma) is further reported. The ω7 PUFA was significantly more cytotoxic in comparison with reference ω6 arachidonic and ω3 eicosapentaenoic acid. A noteworthy non-selective cytotoxicity against normal lung fibroblasts was also established. The paper contains isolation protocols in addition to cytotoxicity data of the isolated compounds. The potential of marine mollusks as a source for rare PUFAs is also discussed. |
format |
Article in Journal/Newspaper |
author |
Espen Hansen Johan Svenson Jeanette H. Andersen Terje Vasskog |
author_facet |
Espen Hansen Johan Svenson Jeanette H. Andersen Terje Vasskog |
author_sort |
Espen Hansen |
title |
Characterization and Cytotoxicity Studies of the Rare 21:4 n-7 Acid and Other Polyunsaturated Fatty Acids from the Marine Opisthobranch Scaphander lignarius, Isolated Using Bioassay Guided Fractionation |
title_short |
Characterization and Cytotoxicity Studies of the Rare 21:4 n-7 Acid and Other Polyunsaturated Fatty Acids from the Marine Opisthobranch Scaphander lignarius, Isolated Using Bioassay Guided Fractionation |
title_full |
Characterization and Cytotoxicity Studies of the Rare 21:4 n-7 Acid and Other Polyunsaturated Fatty Acids from the Marine Opisthobranch Scaphander lignarius, Isolated Using Bioassay Guided Fractionation |
title_fullStr |
Characterization and Cytotoxicity Studies of the Rare 21:4 n-7 Acid and Other Polyunsaturated Fatty Acids from the Marine Opisthobranch Scaphander lignarius, Isolated Using Bioassay Guided Fractionation |
title_full_unstemmed |
Characterization and Cytotoxicity Studies of the Rare 21:4 n-7 Acid and Other Polyunsaturated Fatty Acids from the Marine Opisthobranch Scaphander lignarius, Isolated Using Bioassay Guided Fractionation |
title_sort |
characterization and cytotoxicity studies of the rare 21:4 n-7 acid and other polyunsaturated fatty acids from the marine opisthobranch scaphander lignarius, isolated using bioassay guided fractionation |
publisher |
MDPI AG |
publishDate |
2012 |
url |
https://doi.org/10.3390/md10122676 https://doaj.org/article/d8d93548c8354bf68b291738420b7ab0 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Marine Drugs, Vol 10, Iss 12, Pp 2676-2690 (2012) |
op_relation |
http://www.mdpi.com/1660-3397/10/12/2676 https://doaj.org/toc/1660-3397 doi:10.3390/md10122676 1660-3397 https://doaj.org/article/d8d93548c8354bf68b291738420b7ab0 |
op_doi |
https://doi.org/10.3390/md10122676 |
container_title |
Marine Drugs |
container_volume |
10 |
container_issue |
12 |
container_start_page |
2676 |
op_container_end_page |
2690 |
_version_ |
1766339247146532864 |