Performance of a rapid diagnostic test for the detection of Cryptosporidium spp. in African children admitted to hospital with diarrhea.
Background Cryptosporidium is a protozoan parasite that causes mild to severe diarrhoeal disease in humans. To date, several commercial companies have developed rapid immunoassays for the detection of Cryptosporidium infection. However, the challenge is to identify an accurate, simple and rapid diag...
Published in: | PLOS Neglected Tropical Diseases |
---|---|
Main Authors: | , , , , , , , , , , , , , , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Public Library of Science (PLoS)
2020
|
Subjects: | |
Online Access: | https://doi.org/10.1371/journal.pntd.0008448 https://doaj.org/article/d8d3df87bcda4d09bb6598f72e32b512 |
id |
ftdoajarticles:oai:doaj.org/article:d8d3df87bcda4d09bb6598f72e32b512 |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:d8d3df87bcda4d09bb6598f72e32b512 2023-05-15T15:11:45+02:00 Performance of a rapid diagnostic test for the detection of Cryptosporidium spp. in African children admitted to hospital with diarrhea. Gédéon Prince Manouana Eva Lorenz Mirabeau Mbong Ngwese Paul Alvyn Nguema Moure Oumou Maiga Ascofaré Charity Wiafe Akenten John Amuasi Njari Rakotozandrindrainy Raphael Rakotozandrindrainy Joyce Mbwana John Lusingu Natalie Byrne Sophia Melhem Jeannot Frejus Zinsou Roméo Bayodé Adegbite Benedikt Hogan Doris Winter Jurgen May Peter Gottfried Kremsner Steffen Borrmann Daniel Eibach Ayola Akim Adegnika 2020-07-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0008448 https://doaj.org/article/d8d3df87bcda4d09bb6598f72e32b512 EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pntd.0008448 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0008448 https://doaj.org/article/d8d3df87bcda4d09bb6598f72e32b512 PLoS Neglected Tropical Diseases, Vol 14, Iss 7, p e0008448 (2020) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2020 ftdoajarticles https://doi.org/10.1371/journal.pntd.0008448 2022-12-31T05:48:39Z Background Cryptosporidium is a protozoan parasite that causes mild to severe diarrhoeal disease in humans. To date, several commercial companies have developed rapid immunoassays for the detection of Cryptosporidium infection. However, the challenge is to identify an accurate, simple and rapid diagnostic tool for the estimation of cryptosporidiosis burden. This study aims at evaluating the accuracy of CerTest Crypto, a commercialized rapid diagnostic test (RDT) for the detection of Cryptosporidium antigens in the stool of children presenting with diarrhoea. Methods A cross-sectional study was conducted in four study sites in Sub-Saharan Africa (Gabon, Ghana, Madagascar, and Tanzania), from May 2017 to April 2018. Stool samples were collected from children under 5 years with diarrhoea or a history of diarrhoea within the last 24 hours. All specimens were processed and analyzed using CerTest Crypto RDT against a composite diagnostic panel involving two polymerase chain reaction (PCR) tests (qPCR and RFLP-PCR,) as the gold standard. Results A total of 596 stool samples were collected. Evaluation of the RDT yielded a very low overall sensitivity of 49.6% (confidence interval (CI) 40.1-59.0), a specificity of 92.5% (CI 89.8-94.7), positive predictive value of 61.3% (CI 50.6-71.2), and negative predictive value of 88.5% (85.3-91.1) when compared to the composite reference standard of qPCR and RFLP-PCR for the detection of Cryptosporidium species. Moreover, the performance of this test varied across different sites. Conclusion The weak performance of the studied RDT suggests the need to carefully evaluate available commercial RDTs before their use as standard tools in clinical trials and community survey of Cryptosporidium infections in pediatric cohorts. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 14 7 e0008448 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Gédéon Prince Manouana Eva Lorenz Mirabeau Mbong Ngwese Paul Alvyn Nguema Moure Oumou Maiga Ascofaré Charity Wiafe Akenten John Amuasi Njari Rakotozandrindrainy Raphael Rakotozandrindrainy Joyce Mbwana John Lusingu Natalie Byrne Sophia Melhem Jeannot Frejus Zinsou Roméo Bayodé Adegbite Benedikt Hogan Doris Winter Jurgen May Peter Gottfried Kremsner Steffen Borrmann Daniel Eibach Ayola Akim Adegnika Performance of a rapid diagnostic test for the detection of Cryptosporidium spp. in African children admitted to hospital with diarrhea. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Background Cryptosporidium is a protozoan parasite that causes mild to severe diarrhoeal disease in humans. To date, several commercial companies have developed rapid immunoassays for the detection of Cryptosporidium infection. However, the challenge is to identify an accurate, simple and rapid diagnostic tool for the estimation of cryptosporidiosis burden. This study aims at evaluating the accuracy of CerTest Crypto, a commercialized rapid diagnostic test (RDT) for the detection of Cryptosporidium antigens in the stool of children presenting with diarrhoea. Methods A cross-sectional study was conducted in four study sites in Sub-Saharan Africa (Gabon, Ghana, Madagascar, and Tanzania), from May 2017 to April 2018. Stool samples were collected from children under 5 years with diarrhoea or a history of diarrhoea within the last 24 hours. All specimens were processed and analyzed using CerTest Crypto RDT against a composite diagnostic panel involving two polymerase chain reaction (PCR) tests (qPCR and RFLP-PCR,) as the gold standard. Results A total of 596 stool samples were collected. Evaluation of the RDT yielded a very low overall sensitivity of 49.6% (confidence interval (CI) 40.1-59.0), a specificity of 92.5% (CI 89.8-94.7), positive predictive value of 61.3% (CI 50.6-71.2), and negative predictive value of 88.5% (85.3-91.1) when compared to the composite reference standard of qPCR and RFLP-PCR for the detection of Cryptosporidium species. Moreover, the performance of this test varied across different sites. Conclusion The weak performance of the studied RDT suggests the need to carefully evaluate available commercial RDTs before their use as standard tools in clinical trials and community survey of Cryptosporidium infections in pediatric cohorts. |
format |
Article in Journal/Newspaper |
author |
Gédéon Prince Manouana Eva Lorenz Mirabeau Mbong Ngwese Paul Alvyn Nguema Moure Oumou Maiga Ascofaré Charity Wiafe Akenten John Amuasi Njari Rakotozandrindrainy Raphael Rakotozandrindrainy Joyce Mbwana John Lusingu Natalie Byrne Sophia Melhem Jeannot Frejus Zinsou Roméo Bayodé Adegbite Benedikt Hogan Doris Winter Jurgen May Peter Gottfried Kremsner Steffen Borrmann Daniel Eibach Ayola Akim Adegnika |
author_facet |
Gédéon Prince Manouana Eva Lorenz Mirabeau Mbong Ngwese Paul Alvyn Nguema Moure Oumou Maiga Ascofaré Charity Wiafe Akenten John Amuasi Njari Rakotozandrindrainy Raphael Rakotozandrindrainy Joyce Mbwana John Lusingu Natalie Byrne Sophia Melhem Jeannot Frejus Zinsou Roméo Bayodé Adegbite Benedikt Hogan Doris Winter Jurgen May Peter Gottfried Kremsner Steffen Borrmann Daniel Eibach Ayola Akim Adegnika |
author_sort |
Gédéon Prince Manouana |
title |
Performance of a rapid diagnostic test for the detection of Cryptosporidium spp. in African children admitted to hospital with diarrhea. |
title_short |
Performance of a rapid diagnostic test for the detection of Cryptosporidium spp. in African children admitted to hospital with diarrhea. |
title_full |
Performance of a rapid diagnostic test for the detection of Cryptosporidium spp. in African children admitted to hospital with diarrhea. |
title_fullStr |
Performance of a rapid diagnostic test for the detection of Cryptosporidium spp. in African children admitted to hospital with diarrhea. |
title_full_unstemmed |
Performance of a rapid diagnostic test for the detection of Cryptosporidium spp. in African children admitted to hospital with diarrhea. |
title_sort |
performance of a rapid diagnostic test for the detection of cryptosporidium spp. in african children admitted to hospital with diarrhea. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2020 |
url |
https://doi.org/10.1371/journal.pntd.0008448 https://doaj.org/article/d8d3df87bcda4d09bb6598f72e32b512 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 14, Iss 7, p e0008448 (2020) |
op_relation |
https://doi.org/10.1371/journal.pntd.0008448 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0008448 https://doaj.org/article/d8d3df87bcda4d09bb6598f72e32b512 |
op_doi |
https://doi.org/10.1371/journal.pntd.0008448 |
container_title |
PLOS Neglected Tropical Diseases |
container_volume |
14 |
container_issue |
7 |
container_start_page |
e0008448 |
_version_ |
1766342557327949824 |