Metabolites of Siberian Raspberries: LC-MS Profile, Seasonal Variation, Antioxidant Activity and, Thermal Stability of Rubus matsumuranus Phenolome
Rubus matsumuranus H. Lev. & Vaniot, a famous Siberian shrub of the Rosaceae family, is used in the folk medicine of nomads (Buryats, Yakuts, Soyots, and Mongols) as a remedy for the treatment of diseases of the respiratory and hepatobiliary systems. The lack of scientific information on R. mats...
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MDPI AG
2021
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ftdoajarticles:oai:doaj.org/article:d8c8e71d011d4d2d9de3fd6cab6d9ea8 2023-05-15T18:45:16+02:00 Metabolites of Siberian Raspberries: LC-MS Profile, Seasonal Variation, Antioxidant Activity and, Thermal Stability of Rubus matsumuranus Phenolome Nina I. Kashchenko Daniil N. Olennikov Nadezhda K. Chirikova 2021-10-01T00:00:00Z https://doi.org/10.3390/plants10112317 https://doaj.org/article/d8c8e71d011d4d2d9de3fd6cab6d9ea8 EN eng MDPI AG https://www.mdpi.com/2223-7747/10/11/2317 https://doaj.org/toc/2223-7747 doi:10.3390/plants10112317 2223-7747 https://doaj.org/article/d8c8e71d011d4d2d9de3fd6cab6d9ea8 Plants, Vol 10, Iss 2317, p 2317 (2021) Rubus matsumuranus Rosaceae phenolic compounds ellagitannins high-performance liquid chromatography mass spectrometry Botany QK1-989 article 2021 ftdoajarticles https://doi.org/10.3390/plants10112317 2022-12-31T16:18:02Z Rubus matsumuranus H. Lev. & Vaniot, a famous Siberian shrub of the Rosaceae family, is used in the folk medicine of nomads (Buryats, Yakuts, Soyots, and Mongols) as a remedy for the treatment of diseases of the respiratory and hepatobiliary systems. The lack of scientific information on R. matsumuranus leaves contributed to the investigation of the metabolomic profile and biological activity of this plant. In this study, metabolites of R. matsumuranus leaves in three stages (active growth, flowering, and fruiting) were characterised using high-performance liquid chromatography with photodiode array and electrospray ionisation triple quadrupole mass spectrometric detection (HPLC-PDA-ESI-tQ-MS). In total, 63 compounds were identified, including gallic acid derivatives, hydroxycinnamates, catechins, procyanidins, flavonols, and ellagitannins. Lambertianin C (57.11 mg/g of dry weight, DW), miquelianin (39.63 mg/g DW), and kaempferol-3- O -glucuronide (31.18 mg/g DW) were the major compounds in R. matsumuranus leaves. As a result of the HPLC-PDA-based assay to determine the antioxidant activity, it was revealed that lambertianin A, sanguiin H6, lambertianin C, and sanguiin H11 were effective scavengers of free radicals (2,2-diphenyl-1-picrylhydrazyl, DPPH • ) and possessed Fe 2+ -chelating activity. After an investigation of the phenolic content in infusions and decoctions obtained by extraction with water at different temperatures, it was revealed that a hot infusion (80 °C) is a phenolic-rich preparation of R. matsumuranus leaves. Our research suggests that R. matsumuranus leaves are a rich source of phenolic compounds with high antioxidant properties and that this could be a prospective plant for new functional products. Article in Journal/Newspaper Yakuts Directory of Open Access Journals: DOAJ Articles Plants 10 11 2317 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Rubus matsumuranus Rosaceae phenolic compounds ellagitannins high-performance liquid chromatography mass spectrometry Botany QK1-989 |
spellingShingle |
Rubus matsumuranus Rosaceae phenolic compounds ellagitannins high-performance liquid chromatography mass spectrometry Botany QK1-989 Nina I. Kashchenko Daniil N. Olennikov Nadezhda K. Chirikova Metabolites of Siberian Raspberries: LC-MS Profile, Seasonal Variation, Antioxidant Activity and, Thermal Stability of Rubus matsumuranus Phenolome |
topic_facet |
Rubus matsumuranus Rosaceae phenolic compounds ellagitannins high-performance liquid chromatography mass spectrometry Botany QK1-989 |
description |
Rubus matsumuranus H. Lev. & Vaniot, a famous Siberian shrub of the Rosaceae family, is used in the folk medicine of nomads (Buryats, Yakuts, Soyots, and Mongols) as a remedy for the treatment of diseases of the respiratory and hepatobiliary systems. The lack of scientific information on R. matsumuranus leaves contributed to the investigation of the metabolomic profile and biological activity of this plant. In this study, metabolites of R. matsumuranus leaves in three stages (active growth, flowering, and fruiting) were characterised using high-performance liquid chromatography with photodiode array and electrospray ionisation triple quadrupole mass spectrometric detection (HPLC-PDA-ESI-tQ-MS). In total, 63 compounds were identified, including gallic acid derivatives, hydroxycinnamates, catechins, procyanidins, flavonols, and ellagitannins. Lambertianin C (57.11 mg/g of dry weight, DW), miquelianin (39.63 mg/g DW), and kaempferol-3- O -glucuronide (31.18 mg/g DW) were the major compounds in R. matsumuranus leaves. As a result of the HPLC-PDA-based assay to determine the antioxidant activity, it was revealed that lambertianin A, sanguiin H6, lambertianin C, and sanguiin H11 were effective scavengers of free radicals (2,2-diphenyl-1-picrylhydrazyl, DPPH • ) and possessed Fe 2+ -chelating activity. After an investigation of the phenolic content in infusions and decoctions obtained by extraction with water at different temperatures, it was revealed that a hot infusion (80 °C) is a phenolic-rich preparation of R. matsumuranus leaves. Our research suggests that R. matsumuranus leaves are a rich source of phenolic compounds with high antioxidant properties and that this could be a prospective plant for new functional products. |
format |
Article in Journal/Newspaper |
author |
Nina I. Kashchenko Daniil N. Olennikov Nadezhda K. Chirikova |
author_facet |
Nina I. Kashchenko Daniil N. Olennikov Nadezhda K. Chirikova |
author_sort |
Nina I. Kashchenko |
title |
Metabolites of Siberian Raspberries: LC-MS Profile, Seasonal Variation, Antioxidant Activity and, Thermal Stability of Rubus matsumuranus Phenolome |
title_short |
Metabolites of Siberian Raspberries: LC-MS Profile, Seasonal Variation, Antioxidant Activity and, Thermal Stability of Rubus matsumuranus Phenolome |
title_full |
Metabolites of Siberian Raspberries: LC-MS Profile, Seasonal Variation, Antioxidant Activity and, Thermal Stability of Rubus matsumuranus Phenolome |
title_fullStr |
Metabolites of Siberian Raspberries: LC-MS Profile, Seasonal Variation, Antioxidant Activity and, Thermal Stability of Rubus matsumuranus Phenolome |
title_full_unstemmed |
Metabolites of Siberian Raspberries: LC-MS Profile, Seasonal Variation, Antioxidant Activity and, Thermal Stability of Rubus matsumuranus Phenolome |
title_sort |
metabolites of siberian raspberries: lc-ms profile, seasonal variation, antioxidant activity and, thermal stability of rubus matsumuranus phenolome |
publisher |
MDPI AG |
publishDate |
2021 |
url |
https://doi.org/10.3390/plants10112317 https://doaj.org/article/d8c8e71d011d4d2d9de3fd6cab6d9ea8 |
genre |
Yakuts |
genre_facet |
Yakuts |
op_source |
Plants, Vol 10, Iss 2317, p 2317 (2021) |
op_relation |
https://www.mdpi.com/2223-7747/10/11/2317 https://doaj.org/toc/2223-7747 doi:10.3390/plants10112317 2223-7747 https://doaj.org/article/d8c8e71d011d4d2d9de3fd6cab6d9ea8 |
op_doi |
https://doi.org/10.3390/plants10112317 |
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Plants |
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10 |
container_issue |
11 |
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2317 |
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1766236316488433664 |