Morphogenesis in Trypanosoma cruzi epimastigotes proceeds via a highly asymmetric cell division.
Trypanosoma cruzi is a protist parasite that is the causative agent of Chagas disease, a neglected tropical disease endemic to the Americas. T. cruzi cells are highly polarized and undergo morphological changes as they cycle within their insect and mammalian hosts. Work on related trypanosomatids ha...
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ftdoajarticles:oai:doaj.org/article:d8b6fb3545c64bb49427b4f537353c54 2024-01-14T10:04:53+01:00 Morphogenesis in Trypanosoma cruzi epimastigotes proceeds via a highly asymmetric cell division. Paul C Campbell Christopher L de Graffenried 2023-11-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0011731 https://doaj.org/article/d8b6fb3545c64bb49427b4f537353c54 EN eng Public Library of Science (PLoS) https://journals.plos.org/plosntds/article/file?id=10.1371/journal.pntd.0011731&type=printable https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0011731 https://doaj.org/article/d8b6fb3545c64bb49427b4f537353c54 PLoS Neglected Tropical Diseases, Vol 17, Iss 11, p e0011731 (2023) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2023 ftdoajarticles https://doi.org/10.1371/journal.pntd.0011731 2023-12-17T01:44:39Z Trypanosoma cruzi is a protist parasite that is the causative agent of Chagas disease, a neglected tropical disease endemic to the Americas. T. cruzi cells are highly polarized and undergo morphological changes as they cycle within their insect and mammalian hosts. Work on related trypanosomatids has described cell division mechanisms in several life-cycle stages and identified a set of essential morphogenic proteins that serve as markers for key events during trypanosomatid division. Here, we use Cas9-based tagging of morphogenic genes, live-cell imaging, and expansion microscopy to study the cell division mechanism of the insect-resident epimastigote form of T. cruzi, which represents an understudied trypanosomatid morphotype. We find that T. cruzi epimastigote cell division is highly asymmetric, producing one daughter cell that is significantly smaller than the other. Daughter cell division rates differ by 4.9 h, which may be a consequence of this size disparity. Many of the morphogenic proteins identified in T. brucei have altered localization patterns in T. cruzi epimastigotes, which may reflect fundamental differences in the cell division mechanism of this life cycle stage, which widens and shortens the cell body to accommodate the duplicated organelles and cleavage furrow rather than elongating the cell body along the long axis of the cell, as is the case in life-cycle stages that have been studied in T. brucei. This work provides a foundation for further investigations of T. cruzi cell division and shows that subtle differences in trypanosomatid cell morphology can alter how these parasites divide. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 17 11 e0011731 |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
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English |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Paul C Campbell Christopher L de Graffenried Morphogenesis in Trypanosoma cruzi epimastigotes proceeds via a highly asymmetric cell division. |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Trypanosoma cruzi is a protist parasite that is the causative agent of Chagas disease, a neglected tropical disease endemic to the Americas. T. cruzi cells are highly polarized and undergo morphological changes as they cycle within their insect and mammalian hosts. Work on related trypanosomatids has described cell division mechanisms in several life-cycle stages and identified a set of essential morphogenic proteins that serve as markers for key events during trypanosomatid division. Here, we use Cas9-based tagging of morphogenic genes, live-cell imaging, and expansion microscopy to study the cell division mechanism of the insect-resident epimastigote form of T. cruzi, which represents an understudied trypanosomatid morphotype. We find that T. cruzi epimastigote cell division is highly asymmetric, producing one daughter cell that is significantly smaller than the other. Daughter cell division rates differ by 4.9 h, which may be a consequence of this size disparity. Many of the morphogenic proteins identified in T. brucei have altered localization patterns in T. cruzi epimastigotes, which may reflect fundamental differences in the cell division mechanism of this life cycle stage, which widens and shortens the cell body to accommodate the duplicated organelles and cleavage furrow rather than elongating the cell body along the long axis of the cell, as is the case in life-cycle stages that have been studied in T. brucei. This work provides a foundation for further investigations of T. cruzi cell division and shows that subtle differences in trypanosomatid cell morphology can alter how these parasites divide. |
format |
Article in Journal/Newspaper |
author |
Paul C Campbell Christopher L de Graffenried |
author_facet |
Paul C Campbell Christopher L de Graffenried |
author_sort |
Paul C Campbell |
title |
Morphogenesis in Trypanosoma cruzi epimastigotes proceeds via a highly asymmetric cell division. |
title_short |
Morphogenesis in Trypanosoma cruzi epimastigotes proceeds via a highly asymmetric cell division. |
title_full |
Morphogenesis in Trypanosoma cruzi epimastigotes proceeds via a highly asymmetric cell division. |
title_fullStr |
Morphogenesis in Trypanosoma cruzi epimastigotes proceeds via a highly asymmetric cell division. |
title_full_unstemmed |
Morphogenesis in Trypanosoma cruzi epimastigotes proceeds via a highly asymmetric cell division. |
title_sort |
morphogenesis in trypanosoma cruzi epimastigotes proceeds via a highly asymmetric cell division. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2023 |
url |
https://doi.org/10.1371/journal.pntd.0011731 https://doaj.org/article/d8b6fb3545c64bb49427b4f537353c54 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 17, Iss 11, p e0011731 (2023) |
op_relation |
https://journals.plos.org/plosntds/article/file?id=10.1371/journal.pntd.0011731&type=printable https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0011731 https://doaj.org/article/d8b6fb3545c64bb49427b4f537353c54 |
op_doi |
https://doi.org/10.1371/journal.pntd.0011731 |
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PLOS Neglected Tropical Diseases |
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11 |
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e0011731 |
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