Piscine Orthoreovirus-1 (PRV-1) Has Been Present in Chilean Salmon Aquaculture since at Least 1994
Heart and skeletal muscle inflammation (HSMI) caused by Piscine orthoreovirus (PRV) was first described in farmed Atlantic salmon in Chile in 2011. However, as PRV induces long-lasting infections, it is not known when Chilean farmed salmon may have started to show PRV positivity. This study aimed to...
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ftdoajarticles:oai:doaj.org/article:d8af526a012145839567d842092e43e7 2023-06-11T04:10:15+02:00 Piscine Orthoreovirus-1 (PRV-1) Has Been Present in Chilean Salmon Aquaculture since at Least 1994 Marco Rozas-Serri Ricardo Ildefonso Victoria Jaramillo Estefanía Peñaloza Camila Leiva Soraya Barrientos Darling Coñuecar Lucerina Maldonado Ariel Muñoz Andrea Peña Felipe Aranis Carolina Senn 2023-04-01T00:00:00Z https://doi.org/10.3390/fishes8050229 https://doaj.org/article/d8af526a012145839567d842092e43e7 EN eng MDPI AG https://www.mdpi.com/2410-3888/8/5/229 https://doaj.org/toc/2410-3888 doi:10.3390/fishes8050229 2410-3888 https://doaj.org/article/d8af526a012145839567d842092e43e7 Fishes, Vol 8, Iss 229, p 229 (2023) Atlantic salmon PRV-1 PRV-1a HSMI FFPE Chile Biology (General) QH301-705.5 Genetics QH426-470 article 2023 ftdoajarticles https://doi.org/10.3390/fishes8050229 2023-05-28T00:34:09Z Heart and skeletal muscle inflammation (HSMI) caused by Piscine orthoreovirus (PRV) was first described in farmed Atlantic salmon in Chile in 2011. However, as PRV induces long-lasting infections, it is not known when Chilean farmed salmon may have started to show PRV positivity. This study aimed to evaluate the presence/absence of PRV-1 in formalin-fixed, paraffin-embedded Atlantic salmon heart tissues (FFPE) cultured in Chile during 1992 and 1999. The most frequent histopathological findings in the 42 FFPE blocks were mild focal cardiomyocyte degeneration (57.1%) and a mild focal mononuclear inflammatory infiltrate (21.4%) in the ventricular stratum spongiosum of the heart. One of the 42 heart samples analyzed by RT-qPCR was positive for PRV-1 (2.4%). All samples were negative for other viral and bacterial pathogens that can induce similar histological changes in the heart. Taken together, our results show that PRV-1 has been present in Chile—as a low-virulence genogroup—since at least 1994, 17 years before the first HSMI outbreak in 2011. Finally, archaeovirology can be a valid alternative to contribute to the understanding of the epidemiology of diseases in aquaculture. Article in Journal/Newspaper Atlantic salmon Directory of Open Access Journals: DOAJ Articles Fishes 8 5 229 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Atlantic salmon PRV-1 PRV-1a HSMI FFPE Chile Biology (General) QH301-705.5 Genetics QH426-470 |
spellingShingle |
Atlantic salmon PRV-1 PRV-1a HSMI FFPE Chile Biology (General) QH301-705.5 Genetics QH426-470 Marco Rozas-Serri Ricardo Ildefonso Victoria Jaramillo Estefanía Peñaloza Camila Leiva Soraya Barrientos Darling Coñuecar Lucerina Maldonado Ariel Muñoz Andrea Peña Felipe Aranis Carolina Senn Piscine Orthoreovirus-1 (PRV-1) Has Been Present in Chilean Salmon Aquaculture since at Least 1994 |
topic_facet |
Atlantic salmon PRV-1 PRV-1a HSMI FFPE Chile Biology (General) QH301-705.5 Genetics QH426-470 |
description |
Heart and skeletal muscle inflammation (HSMI) caused by Piscine orthoreovirus (PRV) was first described in farmed Atlantic salmon in Chile in 2011. However, as PRV induces long-lasting infections, it is not known when Chilean farmed salmon may have started to show PRV positivity. This study aimed to evaluate the presence/absence of PRV-1 in formalin-fixed, paraffin-embedded Atlantic salmon heart tissues (FFPE) cultured in Chile during 1992 and 1999. The most frequent histopathological findings in the 42 FFPE blocks were mild focal cardiomyocyte degeneration (57.1%) and a mild focal mononuclear inflammatory infiltrate (21.4%) in the ventricular stratum spongiosum of the heart. One of the 42 heart samples analyzed by RT-qPCR was positive for PRV-1 (2.4%). All samples were negative for other viral and bacterial pathogens that can induce similar histological changes in the heart. Taken together, our results show that PRV-1 has been present in Chile—as a low-virulence genogroup—since at least 1994, 17 years before the first HSMI outbreak in 2011. Finally, archaeovirology can be a valid alternative to contribute to the understanding of the epidemiology of diseases in aquaculture. |
format |
Article in Journal/Newspaper |
author |
Marco Rozas-Serri Ricardo Ildefonso Victoria Jaramillo Estefanía Peñaloza Camila Leiva Soraya Barrientos Darling Coñuecar Lucerina Maldonado Ariel Muñoz Andrea Peña Felipe Aranis Carolina Senn |
author_facet |
Marco Rozas-Serri Ricardo Ildefonso Victoria Jaramillo Estefanía Peñaloza Camila Leiva Soraya Barrientos Darling Coñuecar Lucerina Maldonado Ariel Muñoz Andrea Peña Felipe Aranis Carolina Senn |
author_sort |
Marco Rozas-Serri |
title |
Piscine Orthoreovirus-1 (PRV-1) Has Been Present in Chilean Salmon Aquaculture since at Least 1994 |
title_short |
Piscine Orthoreovirus-1 (PRV-1) Has Been Present in Chilean Salmon Aquaculture since at Least 1994 |
title_full |
Piscine Orthoreovirus-1 (PRV-1) Has Been Present in Chilean Salmon Aquaculture since at Least 1994 |
title_fullStr |
Piscine Orthoreovirus-1 (PRV-1) Has Been Present in Chilean Salmon Aquaculture since at Least 1994 |
title_full_unstemmed |
Piscine Orthoreovirus-1 (PRV-1) Has Been Present in Chilean Salmon Aquaculture since at Least 1994 |
title_sort |
piscine orthoreovirus-1 (prv-1) has been present in chilean salmon aquaculture since at least 1994 |
publisher |
MDPI AG |
publishDate |
2023 |
url |
https://doi.org/10.3390/fishes8050229 https://doaj.org/article/d8af526a012145839567d842092e43e7 |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_source |
Fishes, Vol 8, Iss 229, p 229 (2023) |
op_relation |
https://www.mdpi.com/2410-3888/8/5/229 https://doaj.org/toc/2410-3888 doi:10.3390/fishes8050229 2410-3888 https://doaj.org/article/d8af526a012145839567d842092e43e7 |
op_doi |
https://doi.org/10.3390/fishes8050229 |
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8 |
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5 |
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229 |
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1768384546490810368 |