Phylogenetic Diversity and Biological Activity of Actinobacteria Isolated from the Chukchi Shelf Marine Sediments in the Arctic Ocean
Marine environments are a rich source of Actinobacteria and have the potential to produce a wide variety of biologically active secondary metabolites. In this study, we used four selective isolation media to culture Actinobacteria from the sediments collected from the Chukchi Shelf in the Arctic Oce...
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ftdoajarticles:oai:doaj.org/article:d891b06cde934748b277be9b2c580197 2023-05-15T14:53:31+02:00 Phylogenetic Diversity and Biological Activity of Actinobacteria Isolated from the Chukchi Shelf Marine Sediments in the Arctic Ocean Meng Yuan Yong Yu Hui-Rong Li Ning Dong Xiao-Hua Zhang 2014-03-01T00:00:00Z https://doi.org/10.3390/md12031281 https://doaj.org/article/d891b06cde934748b277be9b2c580197 EN eng MDPI AG http://www.mdpi.com/1660-3397/12/3/1281 https://doaj.org/toc/1660-3397 1660-3397 doi:10.3390/md12031281 https://doaj.org/article/d891b06cde934748b277be9b2c580197 Marine Drugs, Vol 12, Iss 3, Pp 1281-1297 (2014) marine Actinobacteria phylogenetic diversity biological activity Arctic Ocean Biology (General) QH301-705.5 article 2014 ftdoajarticles https://doi.org/10.3390/md12031281 2022-12-30T19:55:21Z Marine environments are a rich source of Actinobacteria and have the potential to produce a wide variety of biologically active secondary metabolites. In this study, we used four selective isolation media to culture Actinobacteria from the sediments collected from the Chukchi Shelf in the Arctic Ocean. A total of 73 actinobacterial strains were isolated. Based on repetitive DNA fingerprinting analysis, we selected 30 representatives for partial characterization according to their phylogenetic diversity, antimicrobial activities and secondary-metabolite biosynthesis genes. Results from the 16S rRNA gene sequence analysis indicated that the 30 strains could be sorted into 18 phylotypes belonging to 14 different genera: Agrococcus, Arsenicicoccus, Arthrobacter, Brevibacterium, Citricoccus, Janibacter, Kocuria, Microbacterium, Microlunatus, Nocardioides, Nocardiopsis, Saccharopolyspora, Salinibacterium and Streptomyces. To our knowledge, this paper is the first report on the isolation of Microlunatus genus members from marine habitats. Of the 30 isolates, 11 strains exhibited antibacterial and/or antifungal activity, seven of which have activities against Bacillus subtilis and Candida albicans. All 30 strains have at least two biosynthetic genes, one-third of which possess more than four biosynthetic genes. This study demonstrates the significant diversity of Actinobacteria in the Chukchi Shelf sediment and their potential for producing biologically active compounds and novel material for genetic manipulation or combinatorial biosynthesis. Article in Journal/Newspaper Arctic Arctic Ocean Chukchi Directory of Open Access Journals: DOAJ Articles Arctic Arctic Ocean Chukchi Shelf ENVELOPE(-169.167,-169.167,70.550,70.550) Marine Drugs 12 3 1281 1297 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
marine Actinobacteria phylogenetic diversity biological activity Arctic Ocean Biology (General) QH301-705.5 |
spellingShingle |
marine Actinobacteria phylogenetic diversity biological activity Arctic Ocean Biology (General) QH301-705.5 Meng Yuan Yong Yu Hui-Rong Li Ning Dong Xiao-Hua Zhang Phylogenetic Diversity and Biological Activity of Actinobacteria Isolated from the Chukchi Shelf Marine Sediments in the Arctic Ocean |
topic_facet |
marine Actinobacteria phylogenetic diversity biological activity Arctic Ocean Biology (General) QH301-705.5 |
description |
Marine environments are a rich source of Actinobacteria and have the potential to produce a wide variety of biologically active secondary metabolites. In this study, we used four selective isolation media to culture Actinobacteria from the sediments collected from the Chukchi Shelf in the Arctic Ocean. A total of 73 actinobacterial strains were isolated. Based on repetitive DNA fingerprinting analysis, we selected 30 representatives for partial characterization according to their phylogenetic diversity, antimicrobial activities and secondary-metabolite biosynthesis genes. Results from the 16S rRNA gene sequence analysis indicated that the 30 strains could be sorted into 18 phylotypes belonging to 14 different genera: Agrococcus, Arsenicicoccus, Arthrobacter, Brevibacterium, Citricoccus, Janibacter, Kocuria, Microbacterium, Microlunatus, Nocardioides, Nocardiopsis, Saccharopolyspora, Salinibacterium and Streptomyces. To our knowledge, this paper is the first report on the isolation of Microlunatus genus members from marine habitats. Of the 30 isolates, 11 strains exhibited antibacterial and/or antifungal activity, seven of which have activities against Bacillus subtilis and Candida albicans. All 30 strains have at least two biosynthetic genes, one-third of which possess more than four biosynthetic genes. This study demonstrates the significant diversity of Actinobacteria in the Chukchi Shelf sediment and their potential for producing biologically active compounds and novel material for genetic manipulation or combinatorial biosynthesis. |
format |
Article in Journal/Newspaper |
author |
Meng Yuan Yong Yu Hui-Rong Li Ning Dong Xiao-Hua Zhang |
author_facet |
Meng Yuan Yong Yu Hui-Rong Li Ning Dong Xiao-Hua Zhang |
author_sort |
Meng Yuan |
title |
Phylogenetic Diversity and Biological Activity of Actinobacteria Isolated from the Chukchi Shelf Marine Sediments in the Arctic Ocean |
title_short |
Phylogenetic Diversity and Biological Activity of Actinobacteria Isolated from the Chukchi Shelf Marine Sediments in the Arctic Ocean |
title_full |
Phylogenetic Diversity and Biological Activity of Actinobacteria Isolated from the Chukchi Shelf Marine Sediments in the Arctic Ocean |
title_fullStr |
Phylogenetic Diversity and Biological Activity of Actinobacteria Isolated from the Chukchi Shelf Marine Sediments in the Arctic Ocean |
title_full_unstemmed |
Phylogenetic Diversity and Biological Activity of Actinobacteria Isolated from the Chukchi Shelf Marine Sediments in the Arctic Ocean |
title_sort |
phylogenetic diversity and biological activity of actinobacteria isolated from the chukchi shelf marine sediments in the arctic ocean |
publisher |
MDPI AG |
publishDate |
2014 |
url |
https://doi.org/10.3390/md12031281 https://doaj.org/article/d891b06cde934748b277be9b2c580197 |
long_lat |
ENVELOPE(-169.167,-169.167,70.550,70.550) |
geographic |
Arctic Arctic Ocean Chukchi Shelf |
geographic_facet |
Arctic Arctic Ocean Chukchi Shelf |
genre |
Arctic Arctic Ocean Chukchi |
genre_facet |
Arctic Arctic Ocean Chukchi |
op_source |
Marine Drugs, Vol 12, Iss 3, Pp 1281-1297 (2014) |
op_relation |
http://www.mdpi.com/1660-3397/12/3/1281 https://doaj.org/toc/1660-3397 1660-3397 doi:10.3390/md12031281 https://doaj.org/article/d891b06cde934748b277be9b2c580197 |
op_doi |
https://doi.org/10.3390/md12031281 |
container_title |
Marine Drugs |
container_volume |
12 |
container_issue |
3 |
container_start_page |
1281 |
op_container_end_page |
1297 |
_version_ |
1766325107863584768 |