Molecular analysis of endocrine disruption in hornyhead turbot at wastewater outfalls in southern california using a second generation multi-species microarray.
Sentinel fish hornyhead turbot (Pleuronichthysverticalis) captured near wastewater outfalls are used for monitoring exposure to industrial and agricultural chemicals of ~ 20 million people living in coastal Southern California. Although analyses of hormones in blood and organ morphology and histolog...
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ftdoajarticles:oai:doaj.org/article:d7f4cc2f1de044878a990396e4372f00 2023-05-15T18:40:58+02:00 Molecular analysis of endocrine disruption in hornyhead turbot at wastewater outfalls in southern california using a second generation multi-species microarray. Michael E Baker Doris E Vidal-Dorsch Cataldo Ribecco L James Sprague Mila Angert Narimene Lekmine Colleen Ludka Andrea Martella Eugenia Ricciardelli Steven M Bay Joseph R Gully Kevin M Kelley Daniel Schlenk Oliana Carnevali Roman Šášik Gary Hardiman 2013-01-01T00:00:00Z https://doi.org/10.1371/journal.pone.0075553 https://doaj.org/article/d7f4cc2f1de044878a990396e4372f00 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC3783431?pdf=render https://doaj.org/toc/1932-6203 1932-6203 doi:10.1371/journal.pone.0075553 https://doaj.org/article/d7f4cc2f1de044878a990396e4372f00 PLoS ONE, Vol 8, Iss 9, p e75553 (2013) Medicine R Science Q article 2013 ftdoajarticles https://doi.org/10.1371/journal.pone.0075553 2022-12-31T15:18:19Z Sentinel fish hornyhead turbot (Pleuronichthysverticalis) captured near wastewater outfalls are used for monitoring exposure to industrial and agricultural chemicals of ~ 20 million people living in coastal Southern California. Although analyses of hormones in blood and organ morphology and histology are useful for assessing contaminant exposure, there is a need for quantitative and sensitive molecular measurements, since contaminants of emerging concern are known to produce subtle effects. We developed a second generation multi-species microarray with expanded content and sensitivity to investigate endocrine disruption in turbot captured near wastewater outfalls in San Diego, Orange County and Los Angeles California. Analysis of expression of genes involved in hormone [e.g., estrogen, androgen, thyroid] responses and xenobiotic metabolism in turbot livers was correlated with a series of phenotypic end points. Molecular analyses of turbot livers uncovered altered expression of vitellogenin and zona pellucida protein, indicating exposure to one or more estrogenic chemicals, as well as, alterations in cytochrome P450 (CYP) 1A, CYP3A and glutathione S-transferase-α indicating induction of the detoxification response. Molecular responses indicative of exposure to endocrine disruptors were observed in field-caught hornyhead turbot captured in Southern California demonstrating the utility of molecular methods for monitoring environmental chemicals in wastewater outfalls. Moreover, this approach can be adapted to monitor other sites for contaminants of emerging concern in other fish species for which there are few available gene sequences. Article in Journal/Newspaper Turbot Directory of Open Access Journals: DOAJ Articles PLoS ONE 8 9 e75553 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Medicine R Science Q |
spellingShingle |
Medicine R Science Q Michael E Baker Doris E Vidal-Dorsch Cataldo Ribecco L James Sprague Mila Angert Narimene Lekmine Colleen Ludka Andrea Martella Eugenia Ricciardelli Steven M Bay Joseph R Gully Kevin M Kelley Daniel Schlenk Oliana Carnevali Roman Šášik Gary Hardiman Molecular analysis of endocrine disruption in hornyhead turbot at wastewater outfalls in southern california using a second generation multi-species microarray. |
topic_facet |
Medicine R Science Q |
description |
Sentinel fish hornyhead turbot (Pleuronichthysverticalis) captured near wastewater outfalls are used for monitoring exposure to industrial and agricultural chemicals of ~ 20 million people living in coastal Southern California. Although analyses of hormones in blood and organ morphology and histology are useful for assessing contaminant exposure, there is a need for quantitative and sensitive molecular measurements, since contaminants of emerging concern are known to produce subtle effects. We developed a second generation multi-species microarray with expanded content and sensitivity to investigate endocrine disruption in turbot captured near wastewater outfalls in San Diego, Orange County and Los Angeles California. Analysis of expression of genes involved in hormone [e.g., estrogen, androgen, thyroid] responses and xenobiotic metabolism in turbot livers was correlated with a series of phenotypic end points. Molecular analyses of turbot livers uncovered altered expression of vitellogenin and zona pellucida protein, indicating exposure to one or more estrogenic chemicals, as well as, alterations in cytochrome P450 (CYP) 1A, CYP3A and glutathione S-transferase-α indicating induction of the detoxification response. Molecular responses indicative of exposure to endocrine disruptors were observed in field-caught hornyhead turbot captured in Southern California demonstrating the utility of molecular methods for monitoring environmental chemicals in wastewater outfalls. Moreover, this approach can be adapted to monitor other sites for contaminants of emerging concern in other fish species for which there are few available gene sequences. |
format |
Article in Journal/Newspaper |
author |
Michael E Baker Doris E Vidal-Dorsch Cataldo Ribecco L James Sprague Mila Angert Narimene Lekmine Colleen Ludka Andrea Martella Eugenia Ricciardelli Steven M Bay Joseph R Gully Kevin M Kelley Daniel Schlenk Oliana Carnevali Roman Šášik Gary Hardiman |
author_facet |
Michael E Baker Doris E Vidal-Dorsch Cataldo Ribecco L James Sprague Mila Angert Narimene Lekmine Colleen Ludka Andrea Martella Eugenia Ricciardelli Steven M Bay Joseph R Gully Kevin M Kelley Daniel Schlenk Oliana Carnevali Roman Šášik Gary Hardiman |
author_sort |
Michael E Baker |
title |
Molecular analysis of endocrine disruption in hornyhead turbot at wastewater outfalls in southern california using a second generation multi-species microarray. |
title_short |
Molecular analysis of endocrine disruption in hornyhead turbot at wastewater outfalls in southern california using a second generation multi-species microarray. |
title_full |
Molecular analysis of endocrine disruption in hornyhead turbot at wastewater outfalls in southern california using a second generation multi-species microarray. |
title_fullStr |
Molecular analysis of endocrine disruption in hornyhead turbot at wastewater outfalls in southern california using a second generation multi-species microarray. |
title_full_unstemmed |
Molecular analysis of endocrine disruption in hornyhead turbot at wastewater outfalls in southern california using a second generation multi-species microarray. |
title_sort |
molecular analysis of endocrine disruption in hornyhead turbot at wastewater outfalls in southern california using a second generation multi-species microarray. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2013 |
url |
https://doi.org/10.1371/journal.pone.0075553 https://doaj.org/article/d7f4cc2f1de044878a990396e4372f00 |
genre |
Turbot |
genre_facet |
Turbot |
op_source |
PLoS ONE, Vol 8, Iss 9, p e75553 (2013) |
op_relation |
http://europepmc.org/articles/PMC3783431?pdf=render https://doaj.org/toc/1932-6203 1932-6203 doi:10.1371/journal.pone.0075553 https://doaj.org/article/d7f4cc2f1de044878a990396e4372f00 |
op_doi |
https://doi.org/10.1371/journal.pone.0075553 |
container_title |
PLoS ONE |
container_volume |
8 |
container_issue |
9 |
container_start_page |
e75553 |
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