Development of the First Microsatellite Multiplex PCR Panel for Meagre ( Argyrosomus regius ), a Commercial Aquaculture Species
In this study, a microsatellite-based multiplex PCR panel for meagre ( Argyrosomus regius ) was developed as a useful and single tool in parental assignment and population studies. Twenty-one specific and interspecific microsatellites from different aquaculture species of meagre ( Argyrosomus regius...
Published in: | Fishes |
---|---|
Main Authors: | , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
MDPI AG
2022
|
Subjects: | |
Online Access: | https://doi.org/10.3390/fishes7030117 https://doaj.org/article/d7dc993acba74e78a66fa2e0db447cd9 |
id |
ftdoajarticles:oai:doaj.org/article:d7dc993acba74e78a66fa2e0db447cd9 |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:d7dc993acba74e78a66fa2e0db447cd9 2023-05-15T18:06:06+02:00 Development of the First Microsatellite Multiplex PCR Panel for Meagre ( Argyrosomus regius ), a Commercial Aquaculture Species Antonio Vallecillos Emilio María-Dolores Javier Villa Francisco Miguel Rueda José Carrillo Guillermo Ramis Mohamed Soula Juan Manuel Afonso Eva Armero 2022-05-01T00:00:00Z https://doi.org/10.3390/fishes7030117 https://doaj.org/article/d7dc993acba74e78a66fa2e0db447cd9 EN eng MDPI AG https://www.mdpi.com/2410-3888/7/3/117 https://doaj.org/toc/2410-3888 doi:10.3390/fishes7030117 2410-3888 https://doaj.org/article/d7dc993acba74e78a66fa2e0db447cd9 Fishes, Vol 7, Iss 117, p 117 (2022) meagre ( Argyrosomus regius ) microsatellites parental assignment PCR population Biology (General) QH301-705.5 Genetics QH426-470 article 2022 ftdoajarticles https://doi.org/10.3390/fishes7030117 2022-12-31T03:15:30Z In this study, a microsatellite-based multiplex PCR panel for meagre ( Argyrosomus regius ) was developed as a useful and single tool in parental assignment and population studies. Twenty-one specific and interspecific microsatellites from different aquaculture species of meagre ( Argyrosomus regius ), Japanese meagre ( A. japonicus ), red drum ( Sciaenops ocellatus ) and yellow meagre ( Acoupa weakfish ) were assessed for genetic variability, allelic range and genotype reliability. Finally, a SuperMultiplex for Argyrosomus regius (SMAr) was designed with only the best eight microsatellite markers. The panel assessment was performed using a batch of brood stock from one company and a sample of 616 offspring. It was possible to assign 95% of the offspring to a single pair of parents using the exclusion method. It is therefore considered an easy procedure, and a powerful and low-cost tool for parental assignment to support companies’ breeding programs and to exchange information between research groups. Article in Journal/Newspaper Red drum Sciaenops ocellatus Directory of Open Access Journals: DOAJ Articles Fishes 7 3 117 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
meagre ( Argyrosomus regius ) microsatellites parental assignment PCR population Biology (General) QH301-705.5 Genetics QH426-470 |
spellingShingle |
meagre ( Argyrosomus regius ) microsatellites parental assignment PCR population Biology (General) QH301-705.5 Genetics QH426-470 Antonio Vallecillos Emilio María-Dolores Javier Villa Francisco Miguel Rueda José Carrillo Guillermo Ramis Mohamed Soula Juan Manuel Afonso Eva Armero Development of the First Microsatellite Multiplex PCR Panel for Meagre ( Argyrosomus regius ), a Commercial Aquaculture Species |
topic_facet |
meagre ( Argyrosomus regius ) microsatellites parental assignment PCR population Biology (General) QH301-705.5 Genetics QH426-470 |
description |
In this study, a microsatellite-based multiplex PCR panel for meagre ( Argyrosomus regius ) was developed as a useful and single tool in parental assignment and population studies. Twenty-one specific and interspecific microsatellites from different aquaculture species of meagre ( Argyrosomus regius ), Japanese meagre ( A. japonicus ), red drum ( Sciaenops ocellatus ) and yellow meagre ( Acoupa weakfish ) were assessed for genetic variability, allelic range and genotype reliability. Finally, a SuperMultiplex for Argyrosomus regius (SMAr) was designed with only the best eight microsatellite markers. The panel assessment was performed using a batch of brood stock from one company and a sample of 616 offspring. It was possible to assign 95% of the offspring to a single pair of parents using the exclusion method. It is therefore considered an easy procedure, and a powerful and low-cost tool for parental assignment to support companies’ breeding programs and to exchange information between research groups. |
format |
Article in Journal/Newspaper |
author |
Antonio Vallecillos Emilio María-Dolores Javier Villa Francisco Miguel Rueda José Carrillo Guillermo Ramis Mohamed Soula Juan Manuel Afonso Eva Armero |
author_facet |
Antonio Vallecillos Emilio María-Dolores Javier Villa Francisco Miguel Rueda José Carrillo Guillermo Ramis Mohamed Soula Juan Manuel Afonso Eva Armero |
author_sort |
Antonio Vallecillos |
title |
Development of the First Microsatellite Multiplex PCR Panel for Meagre ( Argyrosomus regius ), a Commercial Aquaculture Species |
title_short |
Development of the First Microsatellite Multiplex PCR Panel for Meagre ( Argyrosomus regius ), a Commercial Aquaculture Species |
title_full |
Development of the First Microsatellite Multiplex PCR Panel for Meagre ( Argyrosomus regius ), a Commercial Aquaculture Species |
title_fullStr |
Development of the First Microsatellite Multiplex PCR Panel for Meagre ( Argyrosomus regius ), a Commercial Aquaculture Species |
title_full_unstemmed |
Development of the First Microsatellite Multiplex PCR Panel for Meagre ( Argyrosomus regius ), a Commercial Aquaculture Species |
title_sort |
development of the first microsatellite multiplex pcr panel for meagre ( argyrosomus regius ), a commercial aquaculture species |
publisher |
MDPI AG |
publishDate |
2022 |
url |
https://doi.org/10.3390/fishes7030117 https://doaj.org/article/d7dc993acba74e78a66fa2e0db447cd9 |
genre |
Red drum Sciaenops ocellatus |
genre_facet |
Red drum Sciaenops ocellatus |
op_source |
Fishes, Vol 7, Iss 117, p 117 (2022) |
op_relation |
https://www.mdpi.com/2410-3888/7/3/117 https://doaj.org/toc/2410-3888 doi:10.3390/fishes7030117 2410-3888 https://doaj.org/article/d7dc993acba74e78a66fa2e0db447cd9 |
op_doi |
https://doi.org/10.3390/fishes7030117 |
container_title |
Fishes |
container_volume |
7 |
container_issue |
3 |
container_start_page |
117 |
_version_ |
1766177685837447168 |