Development of the First Microsatellite Multiplex PCR Panel for Meagre ( Argyrosomus regius ), a Commercial Aquaculture Species
In this study, a microsatellite-based multiplex PCR panel for meagre ( Argyrosomus regius ) was developed as a useful and single tool in parental assignment and population studies. Twenty-one specific and interspecific microsatellites from different aquaculture species of meagre ( Argyrosomus regius...
Published in: | Fishes |
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Main Authors: | , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
MDPI AG
2022
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Subjects: | |
Online Access: | https://doi.org/10.3390/fishes7030117 https://doaj.org/article/d7dc993acba74e78a66fa2e0db447cd9 |
Summary: | In this study, a microsatellite-based multiplex PCR panel for meagre ( Argyrosomus regius ) was developed as a useful and single tool in parental assignment and population studies. Twenty-one specific and interspecific microsatellites from different aquaculture species of meagre ( Argyrosomus regius ), Japanese meagre ( A. japonicus ), red drum ( Sciaenops ocellatus ) and yellow meagre ( Acoupa weakfish ) were assessed for genetic variability, allelic range and genotype reliability. Finally, a SuperMultiplex for Argyrosomus regius (SMAr) was designed with only the best eight microsatellite markers. The panel assessment was performed using a batch of brood stock from one company and a sample of 616 offspring. It was possible to assign 95% of the offspring to a single pair of parents using the exclusion method. It is therefore considered an easy procedure, and a powerful and low-cost tool for parental assignment to support companies’ breeding programs and to exchange information between research groups. |
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