Diagnostic performance of anti-Zika virus IgM, IgAM and IgG ELISAs during co-circulation of Zika, dengue, and chikungunya viruses in Brazil and Venezuela.

Background Serological diagnosis of Zika virus (ZIKV) infection is challenging because of the antibody cross-reactivity among flaviviruses. At the same time, the role of Nucleic Acid Testing (NAT) is limited by the low proportion of symptomatic infections and the low average viral load. Here, we com...

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Published in:PLOS Neglected Tropical Diseases
Main Authors: Ivonne Morales, Kerstin D Rosenberger, Tereza Magalhaes, Clarice N L Morais, Cynthia Braga, Ernesto T A Marques, Guilherme Amaral Calvet, Luana Damasceno, Patricia Brasil, Ana Maria Bispo de Filippis, Adriana Tami, Sarah Bethencourt, Mayling Alvarez, Pedro A Martínez, Maria G Guzman, Bruno Souza Benevides, Andrea Caprara, Nguyen Than Ha Quyen, Cameron P Simmons, Bridget Wills, Xavier de Lamballerie, Jan Felix Drexler, Thomas Jaenisch, IDAMS Clinical Study Group
Format: Article in Journal/Newspaper
Language:English
Published: Public Library of Science (PLoS) 2021
Subjects:
Online Access:https://doi.org/10.1371/journal.pntd.0009336
https://doaj.org/article/d7ce7e8c04a64ebfb68b1297c7176a66
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spelling ftdoajarticles:oai:doaj.org/article:d7ce7e8c04a64ebfb68b1297c7176a66 2023-05-15T15:17:25+02:00 Diagnostic performance of anti-Zika virus IgM, IgAM and IgG ELISAs during co-circulation of Zika, dengue, and chikungunya viruses in Brazil and Venezuela. Ivonne Morales Kerstin D Rosenberger Tereza Magalhaes Clarice N L Morais Cynthia Braga Ernesto T A Marques Guilherme Amaral Calvet Luana Damasceno Patricia Brasil Ana Maria Bispo de Filippis Adriana Tami Sarah Bethencourt Mayling Alvarez Pedro A Martínez Maria G Guzman Bruno Souza Benevides Andrea Caprara Nguyen Than Ha Quyen Cameron P Simmons Bridget Wills Xavier de Lamballerie Jan Felix Drexler Thomas Jaenisch IDAMS Clinical Study Group 2021-04-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0009336 https://doaj.org/article/d7ce7e8c04a64ebfb68b1297c7176a66 EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pntd.0009336 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0009336 https://doaj.org/article/d7ce7e8c04a64ebfb68b1297c7176a66 PLoS Neglected Tropical Diseases, Vol 15, Iss 4, p e0009336 (2021) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2021 ftdoajarticles https://doi.org/10.1371/journal.pntd.0009336 2022-12-31T11:44:52Z Background Serological diagnosis of Zika virus (ZIKV) infection is challenging because of the antibody cross-reactivity among flaviviruses. At the same time, the role of Nucleic Acid Testing (NAT) is limited by the low proportion of symptomatic infections and the low average viral load. Here, we compared the diagnostic performance of commercially available IgM, IgAM, and IgG ELISAs in sequential samples during the ZIKV and chikungunya (CHIKV) epidemics and co-circulation of dengue virus (DENV) in Brazil and Venezuela. Methodology/principal findings Acute (day of illness 1-5) and follow-up (day of illness ≥ 6) blood samples were collected from nine hundred and seven symptomatic patients enrolled in a prospective multicenter study between June 2012 and August 2016. Acute samples were tested by RT-PCR for ZIKV, DENV, and CHIKV. Acute and follow-up samples were tested for IgM, IgAM, and IgG antibodies to ZIKV using commercially available ELISAs. Among follow-up samples with a RT-PCR confirmed ZIKV infection, anti-ZIKV IgAM sensitivity was 93.5% (43/46), while IgM and IgG exhibited sensitivities of 30.3% (10/33) and 72% (18/25), respectively. An additional 24% (26/109) of ZIKV infections were detected via IgAM seroconversion in ZIKV/DENV/CHIKV RT-PCR negative patients. The specificity of anti-ZIKV IgM was estimated at 93% and that of IgAM at 85%. Conclusions/significance Our findings exemplify the challenges of the assessment of test performance for ZIKV serological tests in the real-world setting, during co-circulation of DENV, ZIKV, and CHIKV. However, we can also demonstrate that the IgAM immunoassay exhibits superior sensitivity to detect ZIKV RT-PCR confirmed infections compared to IgG and IgM immunoassays. The IgAM assay also proves to be promising for detection of anti-ZIKV seroconversions in sequential samples, both in ZIKV PCR-positive as well as PCR-negative patients, making this a candidate assay for serological monitoring of pregnant women in future ZIKV outbreaks. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 15 4 e0009336
institution Open Polar
collection Directory of Open Access Journals: DOAJ Articles
op_collection_id ftdoajarticles
language English
topic Arctic medicine. Tropical medicine
RC955-962
Public aspects of medicine
RA1-1270
spellingShingle Arctic medicine. Tropical medicine
RC955-962
Public aspects of medicine
RA1-1270
Ivonne Morales
Kerstin D Rosenberger
Tereza Magalhaes
Clarice N L Morais
Cynthia Braga
Ernesto T A Marques
Guilherme Amaral Calvet
Luana Damasceno
Patricia Brasil
Ana Maria Bispo de Filippis
Adriana Tami
Sarah Bethencourt
Mayling Alvarez
Pedro A Martínez
Maria G Guzman
Bruno Souza Benevides
Andrea Caprara
Nguyen Than Ha Quyen
Cameron P Simmons
Bridget Wills
Xavier de Lamballerie
Jan Felix Drexler
Thomas Jaenisch
IDAMS Clinical Study Group
Diagnostic performance of anti-Zika virus IgM, IgAM and IgG ELISAs during co-circulation of Zika, dengue, and chikungunya viruses in Brazil and Venezuela.
topic_facet Arctic medicine. Tropical medicine
RC955-962
Public aspects of medicine
RA1-1270
description Background Serological diagnosis of Zika virus (ZIKV) infection is challenging because of the antibody cross-reactivity among flaviviruses. At the same time, the role of Nucleic Acid Testing (NAT) is limited by the low proportion of symptomatic infections and the low average viral load. Here, we compared the diagnostic performance of commercially available IgM, IgAM, and IgG ELISAs in sequential samples during the ZIKV and chikungunya (CHIKV) epidemics and co-circulation of dengue virus (DENV) in Brazil and Venezuela. Methodology/principal findings Acute (day of illness 1-5) and follow-up (day of illness ≥ 6) blood samples were collected from nine hundred and seven symptomatic patients enrolled in a prospective multicenter study between June 2012 and August 2016. Acute samples were tested by RT-PCR for ZIKV, DENV, and CHIKV. Acute and follow-up samples were tested for IgM, IgAM, and IgG antibodies to ZIKV using commercially available ELISAs. Among follow-up samples with a RT-PCR confirmed ZIKV infection, anti-ZIKV IgAM sensitivity was 93.5% (43/46), while IgM and IgG exhibited sensitivities of 30.3% (10/33) and 72% (18/25), respectively. An additional 24% (26/109) of ZIKV infections were detected via IgAM seroconversion in ZIKV/DENV/CHIKV RT-PCR negative patients. The specificity of anti-ZIKV IgM was estimated at 93% and that of IgAM at 85%. Conclusions/significance Our findings exemplify the challenges of the assessment of test performance for ZIKV serological tests in the real-world setting, during co-circulation of DENV, ZIKV, and CHIKV. However, we can also demonstrate that the IgAM immunoassay exhibits superior sensitivity to detect ZIKV RT-PCR confirmed infections compared to IgG and IgM immunoassays. The IgAM assay also proves to be promising for detection of anti-ZIKV seroconversions in sequential samples, both in ZIKV PCR-positive as well as PCR-negative patients, making this a candidate assay for serological monitoring of pregnant women in future ZIKV outbreaks.
format Article in Journal/Newspaper
author Ivonne Morales
Kerstin D Rosenberger
Tereza Magalhaes
Clarice N L Morais
Cynthia Braga
Ernesto T A Marques
Guilherme Amaral Calvet
Luana Damasceno
Patricia Brasil
Ana Maria Bispo de Filippis
Adriana Tami
Sarah Bethencourt
Mayling Alvarez
Pedro A Martínez
Maria G Guzman
Bruno Souza Benevides
Andrea Caprara
Nguyen Than Ha Quyen
Cameron P Simmons
Bridget Wills
Xavier de Lamballerie
Jan Felix Drexler
Thomas Jaenisch
IDAMS Clinical Study Group
author_facet Ivonne Morales
Kerstin D Rosenberger
Tereza Magalhaes
Clarice N L Morais
Cynthia Braga
Ernesto T A Marques
Guilherme Amaral Calvet
Luana Damasceno
Patricia Brasil
Ana Maria Bispo de Filippis
Adriana Tami
Sarah Bethencourt
Mayling Alvarez
Pedro A Martínez
Maria G Guzman
Bruno Souza Benevides
Andrea Caprara
Nguyen Than Ha Quyen
Cameron P Simmons
Bridget Wills
Xavier de Lamballerie
Jan Felix Drexler
Thomas Jaenisch
IDAMS Clinical Study Group
author_sort Ivonne Morales
title Diagnostic performance of anti-Zika virus IgM, IgAM and IgG ELISAs during co-circulation of Zika, dengue, and chikungunya viruses in Brazil and Venezuela.
title_short Diagnostic performance of anti-Zika virus IgM, IgAM and IgG ELISAs during co-circulation of Zika, dengue, and chikungunya viruses in Brazil and Venezuela.
title_full Diagnostic performance of anti-Zika virus IgM, IgAM and IgG ELISAs during co-circulation of Zika, dengue, and chikungunya viruses in Brazil and Venezuela.
title_fullStr Diagnostic performance of anti-Zika virus IgM, IgAM and IgG ELISAs during co-circulation of Zika, dengue, and chikungunya viruses in Brazil and Venezuela.
title_full_unstemmed Diagnostic performance of anti-Zika virus IgM, IgAM and IgG ELISAs during co-circulation of Zika, dengue, and chikungunya viruses in Brazil and Venezuela.
title_sort diagnostic performance of anti-zika virus igm, igam and igg elisas during co-circulation of zika, dengue, and chikungunya viruses in brazil and venezuela.
publisher Public Library of Science (PLoS)
publishDate 2021
url https://doi.org/10.1371/journal.pntd.0009336
https://doaj.org/article/d7ce7e8c04a64ebfb68b1297c7176a66
geographic Arctic
geographic_facet Arctic
genre Arctic
genre_facet Arctic
op_source PLoS Neglected Tropical Diseases, Vol 15, Iss 4, p e0009336 (2021)
op_relation https://doi.org/10.1371/journal.pntd.0009336
https://doaj.org/toc/1935-2727
https://doaj.org/toc/1935-2735
1935-2727
1935-2735
doi:10.1371/journal.pntd.0009336
https://doaj.org/article/d7ce7e8c04a64ebfb68b1297c7176a66
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container_title PLOS Neglected Tropical Diseases
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