Keladi candik (Alocasia longiloba Miq.) petiole extracts promote wound healing in a full thickness excision wound model in rats
Objective: To investigate the wound-healing effect of Alocasia longiloba (A. longiloba) petiole extract on wounds in rats. Methods: Twenty-two male Sprague-dawley rats were randomly assigned to receive 10% solcoseryl gel, phosphate buffer saline, 50% ethanol, 95% ethanol and hexane extracts of A. lo...
Published in: | Asian Pacific Journal of Tropical Biomedicine |
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Wolters Kluwer Medknow Publications
2019
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Online Access: | https://doi.org/10.4103/2221-1691.256727 https://doaj.org/article/d7c7e78ad8d54fc8b64e800e4a2f9ed6 |
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ftdoajarticles:oai:doaj.org/article:d7c7e78ad8d54fc8b64e800e4a2f9ed6 2023-05-15T15:11:36+02:00 Keladi candik (Alocasia longiloba Miq.) petiole extracts promote wound healing in a full thickness excision wound model in rats Nurul Hazirah Che Hamzah Arifullah Mohammed KNS Sirajudeen Mohd Asnizam Asari Zulhazman Hamzah Ibrahim Khalivulla Shaik 2019-01-01T00:00:00Z https://doi.org/10.4103/2221-1691.256727 https://doaj.org/article/d7c7e78ad8d54fc8b64e800e4a2f9ed6 EN eng Wolters Kluwer Medknow Publications http://www.apjtb.org/article.asp?issn=2221-1691;year=2019;volume=9;issue=4;spage=140;epage=149;aulast=Che https://doaj.org/toc/2221-1691 2221-1691 doi:10.4103/2221-1691.256727 https://doaj.org/article/d7c7e78ad8d54fc8b64e800e4a2f9ed6 Asian Pacific Journal of Tropical Biomedicine, Vol 9, Iss 4, Pp 140-149 (2019) full thickness excision wound rat model sprague-dawley rat alocasia longiloba petiole extract Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 article 2019 ftdoajarticles https://doi.org/10.4103/2221-1691.256727 2022-12-30T21:02:14Z Objective: To investigate the wound-healing effect of Alocasia longiloba (A. longiloba) petiole extract on wounds in rats. Methods: Twenty-two male Sprague-dawley rats were randomly assigned to receive 10% solcoseryl gel, phosphate buffer saline, 50% ethanol, 95% ethanol and hexane extracts of A. longiloba at 1.5%, 3% and 6% doses, respectively. A full thicknesses wound (6 mm) was created on the dorsal of the rat; and all rats were applied with the extract solutions, 10% solcoseryl gel and phosphate buffer saline once a day topically until day 12. The wound was photographed on day 1, 6 and 12, and the percentage of wound contraction was calculated. On day 12, rats were sacrificed and histological examination of granulation tissue was carried out using haematoxylin & eosin and Masson’s Trichrome stain to determine the wound healing effect. Results: In this study, 6% of 50% and 95% ethanol extracts of A. longiloba showed 82.50% and 82.32% wound contraction, respectively, and were comparable with 10% solcoseryl gel (82.30%). Meanwhile, phosphate buffer saline treated group showed the lowest wound contraction (69.86%). Histological assessment of wound treated with 6% of 95% ethanol extract of A. longiloba showed distinct epidermal and dermal layer, higher proliferation of fibroblast and more angiogenesis with collagen compared to other wound treated groups. Conclusions: A. longiloba petiole extracts have a wound healing potential and 6% of 95% ethanol extract of A. longiloba is more effective. Further studies are required to understand the wound healing mechanism of action of the extract. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Asian Pacific Journal of Tropical Biomedicine 9 4 140 |
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full thickness excision wound rat model sprague-dawley rat alocasia longiloba petiole extract Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
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full thickness excision wound rat model sprague-dawley rat alocasia longiloba petiole extract Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 Nurul Hazirah Che Hamzah Arifullah Mohammed KNS Sirajudeen Mohd Asnizam Asari Zulhazman Hamzah Ibrahim Khalivulla Shaik Keladi candik (Alocasia longiloba Miq.) petiole extracts promote wound healing in a full thickness excision wound model in rats |
topic_facet |
full thickness excision wound rat model sprague-dawley rat alocasia longiloba petiole extract Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
description |
Objective: To investigate the wound-healing effect of Alocasia longiloba (A. longiloba) petiole extract on wounds in rats. Methods: Twenty-two male Sprague-dawley rats were randomly assigned to receive 10% solcoseryl gel, phosphate buffer saline, 50% ethanol, 95% ethanol and hexane extracts of A. longiloba at 1.5%, 3% and 6% doses, respectively. A full thicknesses wound (6 mm) was created on the dorsal of the rat; and all rats were applied with the extract solutions, 10% solcoseryl gel and phosphate buffer saline once a day topically until day 12. The wound was photographed on day 1, 6 and 12, and the percentage of wound contraction was calculated. On day 12, rats were sacrificed and histological examination of granulation tissue was carried out using haematoxylin & eosin and Masson’s Trichrome stain to determine the wound healing effect. Results: In this study, 6% of 50% and 95% ethanol extracts of A. longiloba showed 82.50% and 82.32% wound contraction, respectively, and were comparable with 10% solcoseryl gel (82.30%). Meanwhile, phosphate buffer saline treated group showed the lowest wound contraction (69.86%). Histological assessment of wound treated with 6% of 95% ethanol extract of A. longiloba showed distinct epidermal and dermal layer, higher proliferation of fibroblast and more angiogenesis with collagen compared to other wound treated groups. Conclusions: A. longiloba petiole extracts have a wound healing potential and 6% of 95% ethanol extract of A. longiloba is more effective. Further studies are required to understand the wound healing mechanism of action of the extract. |
format |
Article in Journal/Newspaper |
author |
Nurul Hazirah Che Hamzah Arifullah Mohammed KNS Sirajudeen Mohd Asnizam Asari Zulhazman Hamzah Ibrahim Khalivulla Shaik |
author_facet |
Nurul Hazirah Che Hamzah Arifullah Mohammed KNS Sirajudeen Mohd Asnizam Asari Zulhazman Hamzah Ibrahim Khalivulla Shaik |
author_sort |
Nurul Hazirah Che Hamzah |
title |
Keladi candik (Alocasia longiloba Miq.) petiole extracts promote wound healing in a full thickness excision wound model in rats |
title_short |
Keladi candik (Alocasia longiloba Miq.) petiole extracts promote wound healing in a full thickness excision wound model in rats |
title_full |
Keladi candik (Alocasia longiloba Miq.) petiole extracts promote wound healing in a full thickness excision wound model in rats |
title_fullStr |
Keladi candik (Alocasia longiloba Miq.) petiole extracts promote wound healing in a full thickness excision wound model in rats |
title_full_unstemmed |
Keladi candik (Alocasia longiloba Miq.) petiole extracts promote wound healing in a full thickness excision wound model in rats |
title_sort |
keladi candik (alocasia longiloba miq.) petiole extracts promote wound healing in a full thickness excision wound model in rats |
publisher |
Wolters Kluwer Medknow Publications |
publishDate |
2019 |
url |
https://doi.org/10.4103/2221-1691.256727 https://doaj.org/article/d7c7e78ad8d54fc8b64e800e4a2f9ed6 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Asian Pacific Journal of Tropical Biomedicine, Vol 9, Iss 4, Pp 140-149 (2019) |
op_relation |
http://www.apjtb.org/article.asp?issn=2221-1691;year=2019;volume=9;issue=4;spage=140;epage=149;aulast=Che https://doaj.org/toc/2221-1691 2221-1691 doi:10.4103/2221-1691.256727 https://doaj.org/article/d7c7e78ad8d54fc8b64e800e4a2f9ed6 |
op_doi |
https://doi.org/10.4103/2221-1691.256727 |
container_title |
Asian Pacific Journal of Tropical Biomedicine |
container_volume |
9 |
container_issue |
4 |
container_start_page |
140 |
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1766342435844128768 |