A cytochrome b561 with ferric reductase activity from the parasitic blood fluke, Schistosoma japonicum.
BACKGROUND: Iron has an integral role in numerous cellular reactions and is required by virtually all organisms. In physiological conditions, iron is abundant in a largely insoluble ferric state. Ferric reductases are an essential component of iron uptake by cells, reducing iron to the soluble ferro...
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ftdoajarticles:oai:doaj.org/article:d7b2ea59f98d4c45af8f16a9ab7e5e4d 2023-05-15T15:09:54+02:00 A cytochrome b561 with ferric reductase activity from the parasitic blood fluke, Schistosoma japonicum. Amber Glanfield Donald P McManus Danielle J Smyth Erica M Lovas Alex Loukas Geoffrey N Gobert Malcolm K Jones 2010-01-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0000884 https://doaj.org/article/d7b2ea59f98d4c45af8f16a9ab7e5e4d EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC2982821?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0000884 https://doaj.org/article/d7b2ea59f98d4c45af8f16a9ab7e5e4d PLoS Neglected Tropical Diseases, Vol 4, Iss 11, p e884 (2010) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2010 ftdoajarticles https://doi.org/10.1371/journal.pntd.0000884 2022-12-31T08:53:03Z BACKGROUND: Iron has an integral role in numerous cellular reactions and is required by virtually all organisms. In physiological conditions, iron is abundant in a largely insoluble ferric state. Ferric reductases are an essential component of iron uptake by cells, reducing iron to the soluble ferrous form. Cytochromes b561 (cyts-b561) are a family of ascorbate reducing transmembrane proteins found in most eukaryotic cells. The identification of the ferric reductase duodenal cytochrome b (dcytb) and recent observations that other cyts-b561 may be involved in iron metabolism have opened novel perspectives for elucidating their physiological function. METHODOLOGY/PRINCIPAL FINDINGS: Here we have identified a new member of the cytochrome b561 (Sjcytb561) family in the pathogenic blood fluke Schistosoma japonicum that localises to the outer surface of this parasitic trematode. Heterologous expression of recombinant Sjcyt-b561 in a Saccharomyces cerevisiae mutant strain that lacks plasma membrane ferrireductase activity demonstrated that the molecule could rescue ferric reductase activity in the yeast. SIGNIFICANCE/CONCLUSIONS: This finding of a new member of the cytochrome b561 family further supports the notion that a ferric reductase function is likely for other members of this protein family. Additionally, the localisation of Sjcytb561 in the surface epithelium of these blood-dwelling schistosomes contributes further to our knowledge concerning nutrient acquisition in these parasites and may provide novel targets for therapeutic intervention. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLoS Neglected Tropical Diseases 4 11 e884 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Amber Glanfield Donald P McManus Danielle J Smyth Erica M Lovas Alex Loukas Geoffrey N Gobert Malcolm K Jones A cytochrome b561 with ferric reductase activity from the parasitic blood fluke, Schistosoma japonicum. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
BACKGROUND: Iron has an integral role in numerous cellular reactions and is required by virtually all organisms. In physiological conditions, iron is abundant in a largely insoluble ferric state. Ferric reductases are an essential component of iron uptake by cells, reducing iron to the soluble ferrous form. Cytochromes b561 (cyts-b561) are a family of ascorbate reducing transmembrane proteins found in most eukaryotic cells. The identification of the ferric reductase duodenal cytochrome b (dcytb) and recent observations that other cyts-b561 may be involved in iron metabolism have opened novel perspectives for elucidating their physiological function. METHODOLOGY/PRINCIPAL FINDINGS: Here we have identified a new member of the cytochrome b561 (Sjcytb561) family in the pathogenic blood fluke Schistosoma japonicum that localises to the outer surface of this parasitic trematode. Heterologous expression of recombinant Sjcyt-b561 in a Saccharomyces cerevisiae mutant strain that lacks plasma membrane ferrireductase activity demonstrated that the molecule could rescue ferric reductase activity in the yeast. SIGNIFICANCE/CONCLUSIONS: This finding of a new member of the cytochrome b561 family further supports the notion that a ferric reductase function is likely for other members of this protein family. Additionally, the localisation of Sjcytb561 in the surface epithelium of these blood-dwelling schistosomes contributes further to our knowledge concerning nutrient acquisition in these parasites and may provide novel targets for therapeutic intervention. |
format |
Article in Journal/Newspaper |
author |
Amber Glanfield Donald P McManus Danielle J Smyth Erica M Lovas Alex Loukas Geoffrey N Gobert Malcolm K Jones |
author_facet |
Amber Glanfield Donald P McManus Danielle J Smyth Erica M Lovas Alex Loukas Geoffrey N Gobert Malcolm K Jones |
author_sort |
Amber Glanfield |
title |
A cytochrome b561 with ferric reductase activity from the parasitic blood fluke, Schistosoma japonicum. |
title_short |
A cytochrome b561 with ferric reductase activity from the parasitic blood fluke, Schistosoma japonicum. |
title_full |
A cytochrome b561 with ferric reductase activity from the parasitic blood fluke, Schistosoma japonicum. |
title_fullStr |
A cytochrome b561 with ferric reductase activity from the parasitic blood fluke, Schistosoma japonicum. |
title_full_unstemmed |
A cytochrome b561 with ferric reductase activity from the parasitic blood fluke, Schistosoma japonicum. |
title_sort |
cytochrome b561 with ferric reductase activity from the parasitic blood fluke, schistosoma japonicum. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2010 |
url |
https://doi.org/10.1371/journal.pntd.0000884 https://doaj.org/article/d7b2ea59f98d4c45af8f16a9ab7e5e4d |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 4, Iss 11, p e884 (2010) |
op_relation |
http://europepmc.org/articles/PMC2982821?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0000884 https://doaj.org/article/d7b2ea59f98d4c45af8f16a9ab7e5e4d |
op_doi |
https://doi.org/10.1371/journal.pntd.0000884 |
container_title |
PLoS Neglected Tropical Diseases |
container_volume |
4 |
container_issue |
11 |
container_start_page |
e884 |
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1766340998179323904 |