Expression of Paracrine Effectors in Human Adipose‐Derived Mesenchymal Stem Cells Treated With Plasma From Brown Bears (Ursus arctos)
Adipose‐derived mesenchymal stem cells (ADSCs) are promising candidates for novel cell therapeutic applications. Hibernating brown bears sustain tissue integrity and function via unknown mechanisms, which might be plasma borne. We hypothesized that plasma from hibernating bears may increase the expr...
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ftdoajarticles:oai:doaj.org/article:d7a00a6991174ba6a4734cfe24b39675 2023-05-15T18:42:09+02:00 Expression of Paracrine Effectors in Human Adipose‐Derived Mesenchymal Stem Cells Treated With Plasma From Brown Bears (Ursus arctos) Maria Berg von Linde Karin Johansson Robert Kruse Gisela Helenius Ninos Samano Örjan Friberg Anne Mette Frøbert Ole Fröbert 2021-01-01T00:00:00Z https://doi.org/10.1111/cts.12872 https://doaj.org/article/d7a00a6991174ba6a4734cfe24b39675 EN eng Wiley https://doi.org/10.1111/cts.12872 https://doaj.org/toc/1752-8054 https://doaj.org/toc/1752-8062 1752-8062 1752-8054 doi:10.1111/cts.12872 https://doaj.org/article/d7a00a6991174ba6a4734cfe24b39675 Clinical and Translational Science, Vol 14, Iss 1, Pp 317-325 (2021) Therapeutics. Pharmacology RM1-950 Public aspects of medicine RA1-1270 article 2021 ftdoajarticles https://doi.org/10.1111/cts.12872 2022-12-31T12:12:19Z Adipose‐derived mesenchymal stem cells (ADSCs) are promising candidates for novel cell therapeutic applications. Hibernating brown bears sustain tissue integrity and function via unknown mechanisms, which might be plasma borne. We hypothesized that plasma from hibernating bears may increase the expression of favorable factors from human ADSCs. In an experimental study, ADSCs from patients with ischemic heart disease were treated with interventional media containing plasma from hibernating and active bears, respectively, and with control medium. Extracted RNA from the ADSCs was sequenced using next generation sequencing. Statistical analyses of differentially expressed genes were performed using fold change analysis, pathway analysis, and gene ontology. As a result, we found that genes associated with inflammation, such as IGF1, PGF, IL11, and TGFA, were downregulated by > 10‐fold in ADSCs treated with winter plasma compared with control. Genes important for cardiovascular development, ADM, ANGPTL4, and APOL3, were upregulated in ADSCs when treated with winter plasma compared with summer plasma. ADSCs treated with bear plasma, regardless if it was from hibernating or active bears, showed downregulation of IGF1, PGF, IL11, INHBA, IER3, and HMOX1 compared with control, suggesting reduced cell growth and differentiation. This can be summarized in the conclusion that plasma from hibernating bears suppresses inflammatory genes and activates genes associated with cardiovascular development in human ADSCs. Identifying the involved regulator(s) holds therapeutic potential. Article in Journal/Newspaper Ursus arctos Directory of Open Access Journals: DOAJ Articles Clinical and Translational Science 14 1 317 325 |
institution |
Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Therapeutics. Pharmacology RM1-950 Public aspects of medicine RA1-1270 |
spellingShingle |
Therapeutics. Pharmacology RM1-950 Public aspects of medicine RA1-1270 Maria Berg von Linde Karin Johansson Robert Kruse Gisela Helenius Ninos Samano Örjan Friberg Anne Mette Frøbert Ole Fröbert Expression of Paracrine Effectors in Human Adipose‐Derived Mesenchymal Stem Cells Treated With Plasma From Brown Bears (Ursus arctos) |
topic_facet |
Therapeutics. Pharmacology RM1-950 Public aspects of medicine RA1-1270 |
description |
Adipose‐derived mesenchymal stem cells (ADSCs) are promising candidates for novel cell therapeutic applications. Hibernating brown bears sustain tissue integrity and function via unknown mechanisms, which might be plasma borne. We hypothesized that plasma from hibernating bears may increase the expression of favorable factors from human ADSCs. In an experimental study, ADSCs from patients with ischemic heart disease were treated with interventional media containing plasma from hibernating and active bears, respectively, and with control medium. Extracted RNA from the ADSCs was sequenced using next generation sequencing. Statistical analyses of differentially expressed genes were performed using fold change analysis, pathway analysis, and gene ontology. As a result, we found that genes associated with inflammation, such as IGF1, PGF, IL11, and TGFA, were downregulated by > 10‐fold in ADSCs treated with winter plasma compared with control. Genes important for cardiovascular development, ADM, ANGPTL4, and APOL3, were upregulated in ADSCs when treated with winter plasma compared with summer plasma. ADSCs treated with bear plasma, regardless if it was from hibernating or active bears, showed downregulation of IGF1, PGF, IL11, INHBA, IER3, and HMOX1 compared with control, suggesting reduced cell growth and differentiation. This can be summarized in the conclusion that plasma from hibernating bears suppresses inflammatory genes and activates genes associated with cardiovascular development in human ADSCs. Identifying the involved regulator(s) holds therapeutic potential. |
format |
Article in Journal/Newspaper |
author |
Maria Berg von Linde Karin Johansson Robert Kruse Gisela Helenius Ninos Samano Örjan Friberg Anne Mette Frøbert Ole Fröbert |
author_facet |
Maria Berg von Linde Karin Johansson Robert Kruse Gisela Helenius Ninos Samano Örjan Friberg Anne Mette Frøbert Ole Fröbert |
author_sort |
Maria Berg von Linde |
title |
Expression of Paracrine Effectors in Human Adipose‐Derived Mesenchymal Stem Cells Treated With Plasma From Brown Bears (Ursus arctos) |
title_short |
Expression of Paracrine Effectors in Human Adipose‐Derived Mesenchymal Stem Cells Treated With Plasma From Brown Bears (Ursus arctos) |
title_full |
Expression of Paracrine Effectors in Human Adipose‐Derived Mesenchymal Stem Cells Treated With Plasma From Brown Bears (Ursus arctos) |
title_fullStr |
Expression of Paracrine Effectors in Human Adipose‐Derived Mesenchymal Stem Cells Treated With Plasma From Brown Bears (Ursus arctos) |
title_full_unstemmed |
Expression of Paracrine Effectors in Human Adipose‐Derived Mesenchymal Stem Cells Treated With Plasma From Brown Bears (Ursus arctos) |
title_sort |
expression of paracrine effectors in human adipose‐derived mesenchymal stem cells treated with plasma from brown bears (ursus arctos) |
publisher |
Wiley |
publishDate |
2021 |
url |
https://doi.org/10.1111/cts.12872 https://doaj.org/article/d7a00a6991174ba6a4734cfe24b39675 |
genre |
Ursus arctos |
genre_facet |
Ursus arctos |
op_source |
Clinical and Translational Science, Vol 14, Iss 1, Pp 317-325 (2021) |
op_relation |
https://doi.org/10.1111/cts.12872 https://doaj.org/toc/1752-8054 https://doaj.org/toc/1752-8062 1752-8062 1752-8054 doi:10.1111/cts.12872 https://doaj.org/article/d7a00a6991174ba6a4734cfe24b39675 |
op_doi |
https://doi.org/10.1111/cts.12872 |
container_title |
Clinical and Translational Science |
container_volume |
14 |
container_issue |
1 |
container_start_page |
317 |
op_container_end_page |
325 |
_version_ |
1766231773533962240 |