Murine dendritic cells transcriptional modulation upon Paracoccidioides brasiliensis infection.
Limited information is available regarding the modulation of genes involved in the innate host response to Paracoccidioides brasiliensis, the etiologic agent of paracoccidioidomycosis. Therefore, we sought to characterize, for the first time, the transcriptional profile of murine bone marrow-derived...
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ftdoajarticles:oai:doaj.org/article:d73d2e9558994ec68a2efc4e95edfd9d 2023-05-15T15:13:02+02:00 Murine dendritic cells transcriptional modulation upon Paracoccidioides brasiliensis infection. Aldo H Tavares Lorena S Derengowski Karen S Ferreira Simoneide S Silva Cláudia Macedo Anamélia L Bocca Geraldo A Passos Sandro R Almeida Ildinete Silva-Pereira 2012-01-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0001459 https://doaj.org/article/d73d2e9558994ec68a2efc4e95edfd9d EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC3250510?pdf=render https://doaj.org/toc/1935-2735 1935-2735 doi:10.1371/journal.pntd.0001459 https://doaj.org/article/d73d2e9558994ec68a2efc4e95edfd9d PLoS Neglected Tropical Diseases, Vol 6, Iss 1, p e1459 (2012) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2012 ftdoajarticles https://doi.org/10.1371/journal.pntd.0001459 2022-12-31T11:35:05Z Limited information is available regarding the modulation of genes involved in the innate host response to Paracoccidioides brasiliensis, the etiologic agent of paracoccidioidomycosis. Therefore, we sought to characterize, for the first time, the transcriptional profile of murine bone marrow-derived dendritic cells (DCs) at an early stage following their initial interaction with P. brasiliensis. DCs connect innate and adaptive immunity by recognizing invading pathogens and determining the type of effector T-cell that mediates an immune response. Gene expression profiles were analyzed using microarray and validated using real-time RT-PCR and protein secretion studies. A total of 299 genes were differentially expressed, many of which are involved in immunity, signal transduction, transcription and apoptosis. Genes encoding the cytokines IL-12 and TNF-α, along with the chemokines CCL22, CCL27 and CXCL10, were up-regulated, suggesting that P. brasiliensis induces a potent proinflammatory response in DCs. In contrast, pattern recognition receptor (PRR)-encoding genes, particularly those related to Toll-like receptors, were down-regulated or unchanged. This result prompted us to evaluate the expression profiles of dectin-1 and mannose receptor, two other important fungal PRRs that were not included in the microarray target cDNA sequences. Unlike the mannose receptor, the dectin-1 receptor gene was significantly induced, suggesting that this β-glucan receptor participates in the recognition of P. brasiliensis. We also used a receptor inhibition assay to evaluate the roles of these receptors in coordinating the expression of several immune-related genes in DCs upon fungal exposure. Altogether, our results provide an initial characterization of early host responses to P. brasiliensis and a basis for better understanding the infectious process of this important neglected pathogen. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLoS Neglected Tropical Diseases 6 1 e1459 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Aldo H Tavares Lorena S Derengowski Karen S Ferreira Simoneide S Silva Cláudia Macedo Anamélia L Bocca Geraldo A Passos Sandro R Almeida Ildinete Silva-Pereira Murine dendritic cells transcriptional modulation upon Paracoccidioides brasiliensis infection. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Limited information is available regarding the modulation of genes involved in the innate host response to Paracoccidioides brasiliensis, the etiologic agent of paracoccidioidomycosis. Therefore, we sought to characterize, for the first time, the transcriptional profile of murine bone marrow-derived dendritic cells (DCs) at an early stage following their initial interaction with P. brasiliensis. DCs connect innate and adaptive immunity by recognizing invading pathogens and determining the type of effector T-cell that mediates an immune response. Gene expression profiles were analyzed using microarray and validated using real-time RT-PCR and protein secretion studies. A total of 299 genes were differentially expressed, many of which are involved in immunity, signal transduction, transcription and apoptosis. Genes encoding the cytokines IL-12 and TNF-α, along with the chemokines CCL22, CCL27 and CXCL10, were up-regulated, suggesting that P. brasiliensis induces a potent proinflammatory response in DCs. In contrast, pattern recognition receptor (PRR)-encoding genes, particularly those related to Toll-like receptors, were down-regulated or unchanged. This result prompted us to evaluate the expression profiles of dectin-1 and mannose receptor, two other important fungal PRRs that were not included in the microarray target cDNA sequences. Unlike the mannose receptor, the dectin-1 receptor gene was significantly induced, suggesting that this β-glucan receptor participates in the recognition of P. brasiliensis. We also used a receptor inhibition assay to evaluate the roles of these receptors in coordinating the expression of several immune-related genes in DCs upon fungal exposure. Altogether, our results provide an initial characterization of early host responses to P. brasiliensis and a basis for better understanding the infectious process of this important neglected pathogen. |
format |
Article in Journal/Newspaper |
author |
Aldo H Tavares Lorena S Derengowski Karen S Ferreira Simoneide S Silva Cláudia Macedo Anamélia L Bocca Geraldo A Passos Sandro R Almeida Ildinete Silva-Pereira |
author_facet |
Aldo H Tavares Lorena S Derengowski Karen S Ferreira Simoneide S Silva Cláudia Macedo Anamélia L Bocca Geraldo A Passos Sandro R Almeida Ildinete Silva-Pereira |
author_sort |
Aldo H Tavares |
title |
Murine dendritic cells transcriptional modulation upon Paracoccidioides brasiliensis infection. |
title_short |
Murine dendritic cells transcriptional modulation upon Paracoccidioides brasiliensis infection. |
title_full |
Murine dendritic cells transcriptional modulation upon Paracoccidioides brasiliensis infection. |
title_fullStr |
Murine dendritic cells transcriptional modulation upon Paracoccidioides brasiliensis infection. |
title_full_unstemmed |
Murine dendritic cells transcriptional modulation upon Paracoccidioides brasiliensis infection. |
title_sort |
murine dendritic cells transcriptional modulation upon paracoccidioides brasiliensis infection. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2012 |
url |
https://doi.org/10.1371/journal.pntd.0001459 https://doaj.org/article/d73d2e9558994ec68a2efc4e95edfd9d |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 6, Iss 1, p e1459 (2012) |
op_relation |
http://europepmc.org/articles/PMC3250510?pdf=render https://doaj.org/toc/1935-2735 1935-2735 doi:10.1371/journal.pntd.0001459 https://doaj.org/article/d73d2e9558994ec68a2efc4e95edfd9d |
op_doi |
https://doi.org/10.1371/journal.pntd.0001459 |
container_title |
PLoS Neglected Tropical Diseases |
container_volume |
6 |
container_issue |
1 |
container_start_page |
e1459 |
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1766343631363375104 |