Conjugative Plasmid pPPUT-Tik1-1 from a Permafrost Pseudomonas putida Strain and Its Present-Day Counterparts Inhabiting Environments and Clinics
A novel group of conjugative plasmids of Pseudomonas is characterized. The prototype plasmid pPPUT-Tik1-1 (153,663 bp), isolated from a permafrost strain of P. putida Tik1, carries a defective mercury transposon, Tn 501 , and a streptomycin resistance transposon, Tn 5393. Ten plasmids and 34 contigs...
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ftdoajarticles:oai:doaj.org/article:d6f0d621c4ab4786b4d0bd25dfd34530 2023-10-09T21:55:12+02:00 Conjugative Plasmid pPPUT-Tik1-1 from a Permafrost Pseudomonas putida Strain and Its Present-Day Counterparts Inhabiting Environments and Clinics Olga Maslova Alexey Beletsky Sofia Mindlin Nika Petrova Andrey Mardanov Mayya Petrova 2023-08-01T00:00:00Z https://doi.org/10.3390/ijms241713518 https://doaj.org/article/d6f0d621c4ab4786b4d0bd25dfd34530 EN eng MDPI AG https://www.mdpi.com/1422-0067/24/17/13518 https://doaj.org/toc/1661-6596 https://doaj.org/toc/1422-0067 doi:10.3390/ijms241713518 1422-0067 1661-6596 https://doaj.org/article/d6f0d621c4ab4786b4d0bd25dfd34530 International Journal of Molecular Sciences, Vol 24, Iss 13518, p 13518 (2023) novel group of plasmids plasmid backbone accessory region antibiotic resistance heavy metal resistance conjugation Biology (General) QH301-705.5 Chemistry QD1-999 article 2023 ftdoajarticles https://doi.org/10.3390/ijms241713518 2023-09-10T00:34:58Z A novel group of conjugative plasmids of Pseudomonas is characterized. The prototype plasmid pPPUT-Tik1-1 (153,663 bp), isolated from a permafrost strain of P. putida Tik1, carries a defective mercury transposon, Tn 501 , and a streptomycin resistance transposon, Tn 5393. Ten plasmids and 34 contigs with backbone regions closely related to pPPUT-Tik1-1 have been found in GenBank. Two of these plasmids from clinical strains of P. putida and P. fulva are almost identical to the ancient plasmid. A characteristic feature of this group of plasmids is the presence of two genes encoding the initiators of replication ( repA1 and repA2 ). None of these genes have high similarity with plasmid replication genes belonging to known incompatibility groups. It has been demonstrated that while pPPUT-Tik1-1-like plasmids have homologous backbone regions, they significantly differ by the molecular structure and the predicted functions of their accessory regions. Some of the pPPUT-Tik1-1-related plasmids carry determinants of antibiotic resistance and/or heavy metal salts. Some plasmids are characterized by the ability to degrade xenobiotics. Plasmids related to pPPUT-Tik1-1 are characterized by a narrow host range and are found in various species of the Pseudomonas genus. Interestingly, we also found shorter plasmid variants containing the same replication module, but lacking conjugation genes and containing other structural changes that strongly distinguish them from plasmids related to pPPUT-Tik1-1, indicating that the structure of the replication module cannot be used as the sole criterion for classifying plasmids. Overall, the results suggest that the plasmids of the novel group can be spread using conjugation in environmental and clinical strains of Pseudomonas and may play diverse adaptive functions due to the presence of various accessory regions. Article in Journal/Newspaper permafrost Directory of Open Access Journals: DOAJ Articles International Journal of Molecular Sciences 24 17 13518 |
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topic |
novel group of plasmids plasmid backbone accessory region antibiotic resistance heavy metal resistance conjugation Biology (General) QH301-705.5 Chemistry QD1-999 |
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novel group of plasmids plasmid backbone accessory region antibiotic resistance heavy metal resistance conjugation Biology (General) QH301-705.5 Chemistry QD1-999 Olga Maslova Alexey Beletsky Sofia Mindlin Nika Petrova Andrey Mardanov Mayya Petrova Conjugative Plasmid pPPUT-Tik1-1 from a Permafrost Pseudomonas putida Strain and Its Present-Day Counterparts Inhabiting Environments and Clinics |
topic_facet |
novel group of plasmids plasmid backbone accessory region antibiotic resistance heavy metal resistance conjugation Biology (General) QH301-705.5 Chemistry QD1-999 |
description |
A novel group of conjugative plasmids of Pseudomonas is characterized. The prototype plasmid pPPUT-Tik1-1 (153,663 bp), isolated from a permafrost strain of P. putida Tik1, carries a defective mercury transposon, Tn 501 , and a streptomycin resistance transposon, Tn 5393. Ten plasmids and 34 contigs with backbone regions closely related to pPPUT-Tik1-1 have been found in GenBank. Two of these plasmids from clinical strains of P. putida and P. fulva are almost identical to the ancient plasmid. A characteristic feature of this group of plasmids is the presence of two genes encoding the initiators of replication ( repA1 and repA2 ). None of these genes have high similarity with plasmid replication genes belonging to known incompatibility groups. It has been demonstrated that while pPPUT-Tik1-1-like plasmids have homologous backbone regions, they significantly differ by the molecular structure and the predicted functions of their accessory regions. Some of the pPPUT-Tik1-1-related plasmids carry determinants of antibiotic resistance and/or heavy metal salts. Some plasmids are characterized by the ability to degrade xenobiotics. Plasmids related to pPPUT-Tik1-1 are characterized by a narrow host range and are found in various species of the Pseudomonas genus. Interestingly, we also found shorter plasmid variants containing the same replication module, but lacking conjugation genes and containing other structural changes that strongly distinguish them from plasmids related to pPPUT-Tik1-1, indicating that the structure of the replication module cannot be used as the sole criterion for classifying plasmids. Overall, the results suggest that the plasmids of the novel group can be spread using conjugation in environmental and clinical strains of Pseudomonas and may play diverse adaptive functions due to the presence of various accessory regions. |
format |
Article in Journal/Newspaper |
author |
Olga Maslova Alexey Beletsky Sofia Mindlin Nika Petrova Andrey Mardanov Mayya Petrova |
author_facet |
Olga Maslova Alexey Beletsky Sofia Mindlin Nika Petrova Andrey Mardanov Mayya Petrova |
author_sort |
Olga Maslova |
title |
Conjugative Plasmid pPPUT-Tik1-1 from a Permafrost Pseudomonas putida Strain and Its Present-Day Counterparts Inhabiting Environments and Clinics |
title_short |
Conjugative Plasmid pPPUT-Tik1-1 from a Permafrost Pseudomonas putida Strain and Its Present-Day Counterparts Inhabiting Environments and Clinics |
title_full |
Conjugative Plasmid pPPUT-Tik1-1 from a Permafrost Pseudomonas putida Strain and Its Present-Day Counterparts Inhabiting Environments and Clinics |
title_fullStr |
Conjugative Plasmid pPPUT-Tik1-1 from a Permafrost Pseudomonas putida Strain and Its Present-Day Counterparts Inhabiting Environments and Clinics |
title_full_unstemmed |
Conjugative Plasmid pPPUT-Tik1-1 from a Permafrost Pseudomonas putida Strain and Its Present-Day Counterparts Inhabiting Environments and Clinics |
title_sort |
conjugative plasmid ppput-tik1-1 from a permafrost pseudomonas putida strain and its present-day counterparts inhabiting environments and clinics |
publisher |
MDPI AG |
publishDate |
2023 |
url |
https://doi.org/10.3390/ijms241713518 https://doaj.org/article/d6f0d621c4ab4786b4d0bd25dfd34530 |
genre |
permafrost |
genre_facet |
permafrost |
op_source |
International Journal of Molecular Sciences, Vol 24, Iss 13518, p 13518 (2023) |
op_relation |
https://www.mdpi.com/1422-0067/24/17/13518 https://doaj.org/toc/1661-6596 https://doaj.org/toc/1422-0067 doi:10.3390/ijms241713518 1422-0067 1661-6596 https://doaj.org/article/d6f0d621c4ab4786b4d0bd25dfd34530 |
op_doi |
https://doi.org/10.3390/ijms241713518 |
container_title |
International Journal of Molecular Sciences |
container_volume |
24 |
container_issue |
17 |
container_start_page |
13518 |
_version_ |
1779319028601323520 |