Assessment of drug resistance associated genetic diversity in Mauritanian isolates of Plasmodium vivax reveals limited polymorphism
Abstract Background Plasmodium vivax is the predominant malaria species in northern Mauritania. Molecular data on P. vivax isolates circulating in West Africa are scarce. The present study analysed molecular markers associated with resistance to antifolates (Pvdhfr and Pvdhps), chloroquine (Pvmdr1),...
Published in: | Malaria Journal |
---|---|
Main Authors: | , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
BMC
2018
|
Subjects: | |
Online Access: | https://doi.org/10.1186/s12936-018-2548-2 https://doaj.org/article/d6b7b51472e445c2ae0c17cf18fc91ed |
id |
ftdoajarticles:oai:doaj.org/article:d6b7b51472e445c2ae0c17cf18fc91ed |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:d6b7b51472e445c2ae0c17cf18fc91ed 2023-05-15T15:17:06+02:00 Assessment of drug resistance associated genetic diversity in Mauritanian isolates of Plasmodium vivax reveals limited polymorphism Jemila Mint Deida Yacoub Ould Khalef Emal Mint Semane Mohamed Salem Ould Ahmedou Salem Hervé Bogreau Leonardo Basco Ali Ould Mohamed Salem Boukhary Rachida Tahar 2018-11-01T00:00:00Z https://doi.org/10.1186/s12936-018-2548-2 https://doaj.org/article/d6b7b51472e445c2ae0c17cf18fc91ed EN eng BMC http://link.springer.com/article/10.1186/s12936-018-2548-2 https://doaj.org/toc/1475-2875 doi:10.1186/s12936-018-2548-2 1475-2875 https://doaj.org/article/d6b7b51472e445c2ae0c17cf18fc91ed Malaria Journal, Vol 17, Iss 1, Pp 1-7 (2018) Malaria Sahara Drug resistance Chloroquine Sulfadoxine–pyrimethamine Artemisinin Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2018 ftdoajarticles https://doi.org/10.1186/s12936-018-2548-2 2022-12-31T04:13:56Z Abstract Background Plasmodium vivax is the predominant malaria species in northern Mauritania. Molecular data on P. vivax isolates circulating in West Africa are scarce. The present study analysed molecular markers associated with resistance to antifolates (Pvdhfr and Pvdhps), chloroquine (Pvmdr1), and artemisinin (Pvk12) in P. vivax isolates collected in two cities located in the Saharan zone of Mauritania. Methods Blood samples were obtained from P. vivax-infected patients recruited for chloroquine therapeutic efficacy study in 2013 and febrile patients spontaneously consulting health facilities in Nouakchott and Atar in 2015–2016. Fragments of Pvdhfr (codons 13, 33, 57, 58, 61, 117, and 174), Pvdhps (codons 382, 383, 512, 553, and 585), Pvmdr1 (codons 976 and 1076) and Pvk12 (codon 552) genes were amplified by PCR and sequenced. Results Most of the isolates in Nouakchott (126/154, 81.8%) and Atar (44/45, 97.8%) carried the wild-type Pvdhfr allelic variant (IPFSTSI). In Nouakchott, all mutants (28/154; 18.2%) had double Pvdhfr mutations in positions 58 and 61 (allelic variant IPFRMSI), whereas in Atar only 1 isolate was mutant (S117N, allelic variant IPFSTNI). The wild-type Pvdhps allelic variant (SAKAV) was found in all tested isolates (Nouakchott, n = 93; Atar, n = 37). Few isolates in Nouakchott (5/115, 4.3%) and Atar (3/79, 3.8%) had the mutant Pvmdr1 allele 976F or 1076L, but not both, including in pre-treatment isolates obtained from patients treated successfully with chloroquine. All isolates (59 in Nouakchott and 48 in Atar) carried the wild-type V552 allele in Pvk12. Conclusions Polymorphisms in Pvdhfr, Pvdhps, Pvmdr1, and Pvk12 were limited in P. vivax isolates collected recently in Nouakchott and Atar. Compared to the isolates collected in Nouakchott in 2007–2009, there was no evidence for selection of mutants. The presence of one, but not both, of the two potential markers of chloroquine resistance in Pvmdr1 in pre-treatment isolates did not influence the clinical outcome, putting into question ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Malaria Journal 17 1 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Malaria Sahara Drug resistance Chloroquine Sulfadoxine–pyrimethamine Artemisinin Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
spellingShingle |
Malaria Sahara Drug resistance Chloroquine Sulfadoxine–pyrimethamine Artemisinin Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Jemila Mint Deida Yacoub Ould Khalef Emal Mint Semane Mohamed Salem Ould Ahmedou Salem Hervé Bogreau Leonardo Basco Ali Ould Mohamed Salem Boukhary Rachida Tahar Assessment of drug resistance associated genetic diversity in Mauritanian isolates of Plasmodium vivax reveals limited polymorphism |
topic_facet |
Malaria Sahara Drug resistance Chloroquine Sulfadoxine–pyrimethamine Artemisinin Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Abstract Background Plasmodium vivax is the predominant malaria species in northern Mauritania. Molecular data on P. vivax isolates circulating in West Africa are scarce. The present study analysed molecular markers associated with resistance to antifolates (Pvdhfr and Pvdhps), chloroquine (Pvmdr1), and artemisinin (Pvk12) in P. vivax isolates collected in two cities located in the Saharan zone of Mauritania. Methods Blood samples were obtained from P. vivax-infected patients recruited for chloroquine therapeutic efficacy study in 2013 and febrile patients spontaneously consulting health facilities in Nouakchott and Atar in 2015–2016. Fragments of Pvdhfr (codons 13, 33, 57, 58, 61, 117, and 174), Pvdhps (codons 382, 383, 512, 553, and 585), Pvmdr1 (codons 976 and 1076) and Pvk12 (codon 552) genes were amplified by PCR and sequenced. Results Most of the isolates in Nouakchott (126/154, 81.8%) and Atar (44/45, 97.8%) carried the wild-type Pvdhfr allelic variant (IPFSTSI). In Nouakchott, all mutants (28/154; 18.2%) had double Pvdhfr mutations in positions 58 and 61 (allelic variant IPFRMSI), whereas in Atar only 1 isolate was mutant (S117N, allelic variant IPFSTNI). The wild-type Pvdhps allelic variant (SAKAV) was found in all tested isolates (Nouakchott, n = 93; Atar, n = 37). Few isolates in Nouakchott (5/115, 4.3%) and Atar (3/79, 3.8%) had the mutant Pvmdr1 allele 976F or 1076L, but not both, including in pre-treatment isolates obtained from patients treated successfully with chloroquine. All isolates (59 in Nouakchott and 48 in Atar) carried the wild-type V552 allele in Pvk12. Conclusions Polymorphisms in Pvdhfr, Pvdhps, Pvmdr1, and Pvk12 were limited in P. vivax isolates collected recently in Nouakchott and Atar. Compared to the isolates collected in Nouakchott in 2007–2009, there was no evidence for selection of mutants. The presence of one, but not both, of the two potential markers of chloroquine resistance in Pvmdr1 in pre-treatment isolates did not influence the clinical outcome, putting into question ... |
format |
Article in Journal/Newspaper |
author |
Jemila Mint Deida Yacoub Ould Khalef Emal Mint Semane Mohamed Salem Ould Ahmedou Salem Hervé Bogreau Leonardo Basco Ali Ould Mohamed Salem Boukhary Rachida Tahar |
author_facet |
Jemila Mint Deida Yacoub Ould Khalef Emal Mint Semane Mohamed Salem Ould Ahmedou Salem Hervé Bogreau Leonardo Basco Ali Ould Mohamed Salem Boukhary Rachida Tahar |
author_sort |
Jemila Mint Deida |
title |
Assessment of drug resistance associated genetic diversity in Mauritanian isolates of Plasmodium vivax reveals limited polymorphism |
title_short |
Assessment of drug resistance associated genetic diversity in Mauritanian isolates of Plasmodium vivax reveals limited polymorphism |
title_full |
Assessment of drug resistance associated genetic diversity in Mauritanian isolates of Plasmodium vivax reveals limited polymorphism |
title_fullStr |
Assessment of drug resistance associated genetic diversity in Mauritanian isolates of Plasmodium vivax reveals limited polymorphism |
title_full_unstemmed |
Assessment of drug resistance associated genetic diversity in Mauritanian isolates of Plasmodium vivax reveals limited polymorphism |
title_sort |
assessment of drug resistance associated genetic diversity in mauritanian isolates of plasmodium vivax reveals limited polymorphism |
publisher |
BMC |
publishDate |
2018 |
url |
https://doi.org/10.1186/s12936-018-2548-2 https://doaj.org/article/d6b7b51472e445c2ae0c17cf18fc91ed |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Malaria Journal, Vol 17, Iss 1, Pp 1-7 (2018) |
op_relation |
http://link.springer.com/article/10.1186/s12936-018-2548-2 https://doaj.org/toc/1475-2875 doi:10.1186/s12936-018-2548-2 1475-2875 https://doaj.org/article/d6b7b51472e445c2ae0c17cf18fc91ed |
op_doi |
https://doi.org/10.1186/s12936-018-2548-2 |
container_title |
Malaria Journal |
container_volume |
17 |
container_issue |
1 |
_version_ |
1766347384948785152 |