Transcriptome Dynamics of an Oyster Larval Response to a Conspecific Cue-Mediated Settlement Induction in the Pacific Oyster Crassostrea gigas
The molecular mechanisms underlying the conspecific cue-mediated larval settlement in Crassostrea gigas is not yet fully understood. In this study, we described and compared the transcriptomes of competent pediveligers (Pedi) and conspecific cue-induced postlarvae (PL). A total of 2383 candidate tra...
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ftdoajarticles:oai:doaj.org/article:d69f35e961f142708fad68d102fbc573 2023-05-15T15:58:02+02:00 Transcriptome Dynamics of an Oyster Larval Response to a Conspecific Cue-Mediated Settlement Induction in the Pacific Oyster Crassostrea gigas Mary Grace Sedanza Jalal Alshaweesh Yi-Li Gao Asami Yoshida Hee-Jin Kim Kenichi Yamaguchi Cyril Glenn Satuito 2022-07-01T00:00:00Z https://doi.org/10.3390/d14070559 https://doaj.org/article/d69f35e961f142708fad68d102fbc573 EN eng MDPI AG https://www.mdpi.com/1424-2818/14/7/559 https://doaj.org/toc/1424-2818 doi:10.3390/d14070559 1424-2818 https://doaj.org/article/d69f35e961f142708fad68d102fbc573 Diversity, Vol 14, Iss 559, p 559 (2022) chemical cues gregarious behavior neuroendocrine biomineralization marine invertebrate physiology RNA-seq Biology (General) QH301-705.5 article 2022 ftdoajarticles https://doi.org/10.3390/d14070559 2022-12-30T20:34:52Z The molecular mechanisms underlying the conspecific cue-mediated larval settlement in Crassostrea gigas is not yet fully understood. In this study, we described and compared the transcriptomes of competent pediveligers (Pedi) and conspecific cue-induced postlarvae (PL). A total of 2383 candidate transcripts were identified: 740 upregulated and 1643 downregulated transcripts, after settlement. Gene Ontology analysis revealed active chitin binding, calcium ion binding, and extracellular region processes in both stages. Results showed that the differential expression trend of six candidate transcripts were consistent between the quantitative real-time PCR and transcriptome data. The differential transcript expression related to shell formation showed closely linked dynamics with a gene regulatory network that may involve the interplay of various hormone receptors, neurotransmitters, and neuropeptide receptors working together in a concerted way in the Pedi and PL stages. Our results highlight the transcriptome dynamics underlying the settlement of oysters on conspecific adult shells and demonstrate the potential use of this cue as an attractant for wild and hatchery-grown oyster larval attachment on artificial substrates. It also suggests the possible involvement of an ecdysone signal pathway that may be linked to a neuroendocrine-biomineralization crosstalk in C. gigas settlement. Article in Journal/Newspaper Crassostrea gigas Pacific oyster Directory of Open Access Journals: DOAJ Articles Pacific Diversity 14 7 559 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
chemical cues gregarious behavior neuroendocrine biomineralization marine invertebrate physiology RNA-seq Biology (General) QH301-705.5 |
spellingShingle |
chemical cues gregarious behavior neuroendocrine biomineralization marine invertebrate physiology RNA-seq Biology (General) QH301-705.5 Mary Grace Sedanza Jalal Alshaweesh Yi-Li Gao Asami Yoshida Hee-Jin Kim Kenichi Yamaguchi Cyril Glenn Satuito Transcriptome Dynamics of an Oyster Larval Response to a Conspecific Cue-Mediated Settlement Induction in the Pacific Oyster Crassostrea gigas |
topic_facet |
chemical cues gregarious behavior neuroendocrine biomineralization marine invertebrate physiology RNA-seq Biology (General) QH301-705.5 |
description |
The molecular mechanisms underlying the conspecific cue-mediated larval settlement in Crassostrea gigas is not yet fully understood. In this study, we described and compared the transcriptomes of competent pediveligers (Pedi) and conspecific cue-induced postlarvae (PL). A total of 2383 candidate transcripts were identified: 740 upregulated and 1643 downregulated transcripts, after settlement. Gene Ontology analysis revealed active chitin binding, calcium ion binding, and extracellular region processes in both stages. Results showed that the differential expression trend of six candidate transcripts were consistent between the quantitative real-time PCR and transcriptome data. The differential transcript expression related to shell formation showed closely linked dynamics with a gene regulatory network that may involve the interplay of various hormone receptors, neurotransmitters, and neuropeptide receptors working together in a concerted way in the Pedi and PL stages. Our results highlight the transcriptome dynamics underlying the settlement of oysters on conspecific adult shells and demonstrate the potential use of this cue as an attractant for wild and hatchery-grown oyster larval attachment on artificial substrates. It also suggests the possible involvement of an ecdysone signal pathway that may be linked to a neuroendocrine-biomineralization crosstalk in C. gigas settlement. |
format |
Article in Journal/Newspaper |
author |
Mary Grace Sedanza Jalal Alshaweesh Yi-Li Gao Asami Yoshida Hee-Jin Kim Kenichi Yamaguchi Cyril Glenn Satuito |
author_facet |
Mary Grace Sedanza Jalal Alshaweesh Yi-Li Gao Asami Yoshida Hee-Jin Kim Kenichi Yamaguchi Cyril Glenn Satuito |
author_sort |
Mary Grace Sedanza |
title |
Transcriptome Dynamics of an Oyster Larval Response to a Conspecific Cue-Mediated Settlement Induction in the Pacific Oyster Crassostrea gigas |
title_short |
Transcriptome Dynamics of an Oyster Larval Response to a Conspecific Cue-Mediated Settlement Induction in the Pacific Oyster Crassostrea gigas |
title_full |
Transcriptome Dynamics of an Oyster Larval Response to a Conspecific Cue-Mediated Settlement Induction in the Pacific Oyster Crassostrea gigas |
title_fullStr |
Transcriptome Dynamics of an Oyster Larval Response to a Conspecific Cue-Mediated Settlement Induction in the Pacific Oyster Crassostrea gigas |
title_full_unstemmed |
Transcriptome Dynamics of an Oyster Larval Response to a Conspecific Cue-Mediated Settlement Induction in the Pacific Oyster Crassostrea gigas |
title_sort |
transcriptome dynamics of an oyster larval response to a conspecific cue-mediated settlement induction in the pacific oyster crassostrea gigas |
publisher |
MDPI AG |
publishDate |
2022 |
url |
https://doi.org/10.3390/d14070559 https://doaj.org/article/d69f35e961f142708fad68d102fbc573 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
Diversity, Vol 14, Iss 559, p 559 (2022) |
op_relation |
https://www.mdpi.com/1424-2818/14/7/559 https://doaj.org/toc/1424-2818 doi:10.3390/d14070559 1424-2818 https://doaj.org/article/d69f35e961f142708fad68d102fbc573 |
op_doi |
https://doi.org/10.3390/d14070559 |
container_title |
Diversity |
container_volume |
14 |
container_issue |
7 |
container_start_page |
559 |
_version_ |
1766393753024593920 |