In vitro anticancer activity of polysaccharide extracted from red alga Jania rubens against breast and colon cancer cell lines
Objective: To evaluate the potential role of the polysaccharides of the marine algae as an anticancer agent in vitro against colon cancer cell line (CoCa2) and breast cancer (MCF7) cell lines and to measure lactate dehydrogenase enzyme (LDH) activity as biomarker of membrane integrity of the cells....
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Wolters Kluwer Medknow Publications
2018
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ftdoajarticles:oai:doaj.org/article:d69f125bc2c84647a3067e3f632810e1 2023-05-15T15:14:17+02:00 In vitro anticancer activity of polysaccharide extracted from red alga Jania rubens against breast and colon cancer cell lines Saly Gheda Mostafa El-Sheekh Alaa Abou-Zeid 2018-01-01T00:00:00Z https://doi.org/10.4103/1995-7645.244523 https://doaj.org/article/d69f125bc2c84647a3067e3f632810e1 EN eng Wolters Kluwer Medknow Publications http://www.apjtm.org/article.asp?issn=1995-7645;year=2018;volume=11;issue=10;spage=583;epage=589;aulast=Gheda https://doaj.org/toc/2352-4146 2352-4146 doi:10.4103/1995-7645.244523 https://doaj.org/article/d69f125bc2c84647a3067e3f632810e1 Asian Pacific Journal of Tropical Medicine, Vol 11, Iss 10, Pp 583-589 (2018) marine algae antioxidant activity cytotoxicity apoptosis lactate dehydrogenase polymerase chain reaction Arctic medicine. Tropical medicine RC955-962 article 2018 ftdoajarticles https://doi.org/10.4103/1995-7645.244523 2022-12-31T14:20:10Z Objective: To evaluate the potential role of the polysaccharides of the marine algae as an anticancer agent in vitro against colon cancer cell line (CoCa2) and breast cancer (MCF7) cell lines and to measure lactate dehydrogenase enzyme (LDH) activity as biomarker of membrane integrity of the cells. Methods: The cells of breast cancer (MCF7) and colon cancer (CoCa2) were used to evaluate the potential anticancer role of the polysaccharides of marine algae. Anti-proliferative activity against MCF7 and CoCa2 cell lines were evaluated in vitro by the 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay. Results: The in vitro assay of the antioxidant activity of eight marine seaweed species showed that the red seaweed Jania rubens (J. rubens) had the highest DPPH (2,2 diphenyl-1-picrylhydrazyl) free radical scavenging activity. The extracted polysaccharides with concentrations 0.1–40.0 mg/mL from J. rubens were tested for its anticancer potentiality and cytotoxic effects against the cell lines of human breast (MCF7) and colon cancer (CoCa2) cell lines by MTT assay. The inhibitory concentration at 50 (IC50) value the of J. rubens polysaccharide extract was 0.312 5 mg/mL for MCF7 and 20 mg/mL for CoCa2. LDH activity and annexin V concentration were higher in the treated MCF7 and CaCo2 cells than in the untreated ones. Quantitative polymerase chain reaction technique indicated that the polysaccharide treatments caused up-regulation of Bax, caspase 8 and P53 genes expression in CoCa2 cells, and up-regulation of caspase 3 and down-regulation of Bcl2 genes expression in MCF7 cells. Conclusions: The polysaccharides of the red marine alga J. rubens could be a potential candidate for the natural compounds as antioxidant and anticancer therapy. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Asian Pacific Journal of Tropical Medicine 11 10 583 |
institution |
Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
marine algae antioxidant activity cytotoxicity apoptosis lactate dehydrogenase polymerase chain reaction Arctic medicine. Tropical medicine RC955-962 |
spellingShingle |
marine algae antioxidant activity cytotoxicity apoptosis lactate dehydrogenase polymerase chain reaction Arctic medicine. Tropical medicine RC955-962 Saly Gheda Mostafa El-Sheekh Alaa Abou-Zeid In vitro anticancer activity of polysaccharide extracted from red alga Jania rubens against breast and colon cancer cell lines |
topic_facet |
marine algae antioxidant activity cytotoxicity apoptosis lactate dehydrogenase polymerase chain reaction Arctic medicine. Tropical medicine RC955-962 |
description |
Objective: To evaluate the potential role of the polysaccharides of the marine algae as an anticancer agent in vitro against colon cancer cell line (CoCa2) and breast cancer (MCF7) cell lines and to measure lactate dehydrogenase enzyme (LDH) activity as biomarker of membrane integrity of the cells. Methods: The cells of breast cancer (MCF7) and colon cancer (CoCa2) were used to evaluate the potential anticancer role of the polysaccharides of marine algae. Anti-proliferative activity against MCF7 and CoCa2 cell lines were evaluated in vitro by the 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay. Results: The in vitro assay of the antioxidant activity of eight marine seaweed species showed that the red seaweed Jania rubens (J. rubens) had the highest DPPH (2,2 diphenyl-1-picrylhydrazyl) free radical scavenging activity. The extracted polysaccharides with concentrations 0.1–40.0 mg/mL from J. rubens were tested for its anticancer potentiality and cytotoxic effects against the cell lines of human breast (MCF7) and colon cancer (CoCa2) cell lines by MTT assay. The inhibitory concentration at 50 (IC50) value the of J. rubens polysaccharide extract was 0.312 5 mg/mL for MCF7 and 20 mg/mL for CoCa2. LDH activity and annexin V concentration were higher in the treated MCF7 and CaCo2 cells than in the untreated ones. Quantitative polymerase chain reaction technique indicated that the polysaccharide treatments caused up-regulation of Bax, caspase 8 and P53 genes expression in CoCa2 cells, and up-regulation of caspase 3 and down-regulation of Bcl2 genes expression in MCF7 cells. Conclusions: The polysaccharides of the red marine alga J. rubens could be a potential candidate for the natural compounds as antioxidant and anticancer therapy. |
format |
Article in Journal/Newspaper |
author |
Saly Gheda Mostafa El-Sheekh Alaa Abou-Zeid |
author_facet |
Saly Gheda Mostafa El-Sheekh Alaa Abou-Zeid |
author_sort |
Saly Gheda |
title |
In vitro anticancer activity of polysaccharide extracted from red alga Jania rubens against breast and colon cancer cell lines |
title_short |
In vitro anticancer activity of polysaccharide extracted from red alga Jania rubens against breast and colon cancer cell lines |
title_full |
In vitro anticancer activity of polysaccharide extracted from red alga Jania rubens against breast and colon cancer cell lines |
title_fullStr |
In vitro anticancer activity of polysaccharide extracted from red alga Jania rubens against breast and colon cancer cell lines |
title_full_unstemmed |
In vitro anticancer activity of polysaccharide extracted from red alga Jania rubens against breast and colon cancer cell lines |
title_sort |
in vitro anticancer activity of polysaccharide extracted from red alga jania rubens against breast and colon cancer cell lines |
publisher |
Wolters Kluwer Medknow Publications |
publishDate |
2018 |
url |
https://doi.org/10.4103/1995-7645.244523 https://doaj.org/article/d69f125bc2c84647a3067e3f632810e1 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Asian Pacific Journal of Tropical Medicine, Vol 11, Iss 10, Pp 583-589 (2018) |
op_relation |
http://www.apjtm.org/article.asp?issn=1995-7645;year=2018;volume=11;issue=10;spage=583;epage=589;aulast=Gheda https://doaj.org/toc/2352-4146 2352-4146 doi:10.4103/1995-7645.244523 https://doaj.org/article/d69f125bc2c84647a3067e3f632810e1 |
op_doi |
https://doi.org/10.4103/1995-7645.244523 |
container_title |
Asian Pacific Journal of Tropical Medicine |
container_volume |
11 |
container_issue |
10 |
container_start_page |
583 |
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1766344756247396352 |