Biodiesel Production Using Palm Oil with a MOF-Lipase B Biocatalyst from Candida Antarctica: A Kinetic and Thermodynamic Study
This research presents the results of the immobilization of Candida Antarctica Lipase B (CALB) on MOF-199 and ZIF-8 and its use in the production of biodiesel through the transesterification reaction using African Palm Oil (APO). The results show that the highest adsorption capacity, the 26.9 mg·g −...
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ftdoajarticles:oai:doaj.org/article:d68dd2d219824a9dbf75ec431023636c 2023-07-30T03:59:28+02:00 Biodiesel Production Using Palm Oil with a MOF-Lipase B Biocatalyst from Candida Antarctica: A Kinetic and Thermodynamic Study Liliana Giraldo Fernando Gómez-Granados Juan Carlos Moreno-Piraján 2023-06-01T00:00:00Z https://doi.org/10.3390/ijms241310741 https://doaj.org/article/d68dd2d219824a9dbf75ec431023636c EN eng MDPI AG https://www.mdpi.com/1422-0067/24/13/10741 https://doaj.org/toc/1661-6596 https://doaj.org/toc/1422-0067 doi:10.3390/ijms241310741 1422-0067 1661-6596 https://doaj.org/article/d68dd2d219824a9dbf75ec431023636c International Journal of Molecular Sciences, Vol 24, Iss 10741, p 10741 (2023) Candida Antarctica biodiesel kinetics lipase adsorption thermodynamic Biology (General) QH301-705.5 Chemistry QD1-999 article 2023 ftdoajarticles https://doi.org/10.3390/ijms241310741 2023-07-16T00:35:06Z This research presents the results of the immobilization of Candida Antarctica Lipase B (CALB) on MOF-199 and ZIF-8 and its use in the production of biodiesel through the transesterification reaction using African Palm Oil (APO). The results show that the highest adsorption capacity, the 26.9 mg·g −1 Lipase, was achieved using ZIF-8 at 45 °C and an initial protein concentration of 1.20 mg·mL −1 . The results obtained for the adsorption equilibrium studies allow us to infer that CALB was physically adsorbed on ZIF-8 while chemically adsorbed with MOF-199. It was determined that the adsorption between Lipase and the MOFs under study better fit the Sips isotherm model. The results of the kinetic studies show that adsorption kinetics follow the Elovich model for the two synthesized biocatalysts. This research shows that under the experimental conditions in which the studies were carried out, the adsorption processes are a function of the intraparticle and film diffusion models. According to the results, the prepared biocatalysts showed a high efficiency in the transesterification reaction to produce biodiesel, with methanol as a co-solvent medium. In this work, the catalytic studies for the imidazolate, ZIF-8, presented more catalytic activity when used with CALB. This system presented 95% biodiesel conversion, while the biocatalyst formed by MOF-199 and CALB generated a catalytic conversion percentage of 90%. Although both percentages are high, it should be noted that CALB-MOF-199 presented better reusability, which is due to chemical interactions. Article in Journal/Newspaper Antarc* Antarctica Directory of Open Access Journals: DOAJ Articles International Journal of Molecular Sciences 24 13 10741 |
institution |
Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Candida Antarctica biodiesel kinetics lipase adsorption thermodynamic Biology (General) QH301-705.5 Chemistry QD1-999 |
spellingShingle |
Candida Antarctica biodiesel kinetics lipase adsorption thermodynamic Biology (General) QH301-705.5 Chemistry QD1-999 Liliana Giraldo Fernando Gómez-Granados Juan Carlos Moreno-Piraján Biodiesel Production Using Palm Oil with a MOF-Lipase B Biocatalyst from Candida Antarctica: A Kinetic and Thermodynamic Study |
topic_facet |
Candida Antarctica biodiesel kinetics lipase adsorption thermodynamic Biology (General) QH301-705.5 Chemistry QD1-999 |
description |
This research presents the results of the immobilization of Candida Antarctica Lipase B (CALB) on MOF-199 and ZIF-8 and its use in the production of biodiesel through the transesterification reaction using African Palm Oil (APO). The results show that the highest adsorption capacity, the 26.9 mg·g −1 Lipase, was achieved using ZIF-8 at 45 °C and an initial protein concentration of 1.20 mg·mL −1 . The results obtained for the adsorption equilibrium studies allow us to infer that CALB was physically adsorbed on ZIF-8 while chemically adsorbed with MOF-199. It was determined that the adsorption between Lipase and the MOFs under study better fit the Sips isotherm model. The results of the kinetic studies show that adsorption kinetics follow the Elovich model for the two synthesized biocatalysts. This research shows that under the experimental conditions in which the studies were carried out, the adsorption processes are a function of the intraparticle and film diffusion models. According to the results, the prepared biocatalysts showed a high efficiency in the transesterification reaction to produce biodiesel, with methanol as a co-solvent medium. In this work, the catalytic studies for the imidazolate, ZIF-8, presented more catalytic activity when used with CALB. This system presented 95% biodiesel conversion, while the biocatalyst formed by MOF-199 and CALB generated a catalytic conversion percentage of 90%. Although both percentages are high, it should be noted that CALB-MOF-199 presented better reusability, which is due to chemical interactions. |
format |
Article in Journal/Newspaper |
author |
Liliana Giraldo Fernando Gómez-Granados Juan Carlos Moreno-Piraján |
author_facet |
Liliana Giraldo Fernando Gómez-Granados Juan Carlos Moreno-Piraján |
author_sort |
Liliana Giraldo |
title |
Biodiesel Production Using Palm Oil with a MOF-Lipase B Biocatalyst from Candida Antarctica: A Kinetic and Thermodynamic Study |
title_short |
Biodiesel Production Using Palm Oil with a MOF-Lipase B Biocatalyst from Candida Antarctica: A Kinetic and Thermodynamic Study |
title_full |
Biodiesel Production Using Palm Oil with a MOF-Lipase B Biocatalyst from Candida Antarctica: A Kinetic and Thermodynamic Study |
title_fullStr |
Biodiesel Production Using Palm Oil with a MOF-Lipase B Biocatalyst from Candida Antarctica: A Kinetic and Thermodynamic Study |
title_full_unstemmed |
Biodiesel Production Using Palm Oil with a MOF-Lipase B Biocatalyst from Candida Antarctica: A Kinetic and Thermodynamic Study |
title_sort |
biodiesel production using palm oil with a mof-lipase b biocatalyst from candida antarctica: a kinetic and thermodynamic study |
publisher |
MDPI AG |
publishDate |
2023 |
url |
https://doi.org/10.3390/ijms241310741 https://doaj.org/article/d68dd2d219824a9dbf75ec431023636c |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_source |
International Journal of Molecular Sciences, Vol 24, Iss 10741, p 10741 (2023) |
op_relation |
https://www.mdpi.com/1422-0067/24/13/10741 https://doaj.org/toc/1661-6596 https://doaj.org/toc/1422-0067 doi:10.3390/ijms241310741 1422-0067 1661-6596 https://doaj.org/article/d68dd2d219824a9dbf75ec431023636c |
op_doi |
https://doi.org/10.3390/ijms241310741 |
container_title |
International Journal of Molecular Sciences |
container_volume |
24 |
container_issue |
13 |
container_start_page |
10741 |
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1772810298358497280 |