Transcriptome Profiling of Two Ornamental and Medicinal Papaver Herbs

The Papaver spp. (Papaver rhoeas (Corn poppy) and Papaver nudicaule (Iceland poppy)) genera are ornamental and medicinal plants that are used for the isolation of alkaloid drugs. In this study, we generated 700 Mb of transcriptome sequences with the PacBio platform. They were assembled into 120,926...

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Bibliographic Details
Published in:International Journal of Molecular Sciences
Main Authors: Jaehyeon Oh, Younhee Shin, In Jin Ha, Min Young Lee, Seok-Geun Lee, Byeong-Chul Kang, Dongsoo Kyeong, Dowan Kim
Format: Article in Journal/Newspaper
Language:English
Published: MDPI AG 2018
Subjects:
poppy
Papaver rhoeas
Papaver nudicaule
transcriptome
alkaloid biosynthesis
target metabolome
Biology (General)
QH301-705.5
Chemistry
QD1-999
Bia
Iceland
Online Access:https://doi.org/10.3390/ijms19103192
https://doaj.org/article/d6796d7a25104a97803f8d8e047c56a9
Description
Summary:The Papaver spp. (Papaver rhoeas (Corn poppy) and Papaver nudicaule (Iceland poppy)) genera are ornamental and medicinal plants that are used for the isolation of alkaloid drugs. In this study, we generated 700 Mb of transcriptome sequences with the PacBio platform. They were assembled into 120,926 contigs, and 1185 (82.2%) of the benchmarking universal single-copy orthologs (BUSCO) core genes were completely present in our assembled transcriptome. Furthermore, using 128 Gb of Illumina sequences, the transcript expression was assessed at three stages of Papaver plant development (30, 60, and 90 days), from which we identified 137 differentially expressed transcripts. Furthermore, three co-occurrence heat maps are generated from 51 different plant genomes along with the Papaver transcriptome, i.e., secondary metabolite biosynthesis, isoquinoline alkaloid biosynthesis (BIA) pathway, and cytochrome. Sixty-nine transcripts in the BIA pathway along with 22 different alkaloids (quantified with LC-QTOF-MS/MS) were mapped into the BIA KEGG map (map00950). Finally, we identified 39 full-length cytochrome transcripts and compared them with other genomes. Collectively, this transcriptome data, along with the expression and quantitative metabolite profiles, provides an initial recording of secondary metabolites and their expression related to Papaver plant development. Moreover, these profiles could help to further detail the functional characterization of the various secondary metabolite biosynthesis and Papaver plant development associated problems.

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