DNA sequencing confirms meningeal worm (Parelaphostrongylus tenuis) and muscle worm (Parelaphostrongylus andersoni) in white-tailed deer (Odocoileus virginianus): Implications for moose (Alces alces) management
In North America, some moose populations are declining, and meningeal worm (Parelaphostrongylus tenuis) infections may be contributing. Moose are aberrant hosts for meningeal worm and develop severe pathology whereas white-tailed deer (WTD) are definitive hosts that experience minimal pathology and...
Published in: | International Journal for Parasitology: Parasites and Wildlife |
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Language: | English |
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2023
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Online Access: | https://doi.org/10.1016/j.ijppaw.2023.01.009 https://doaj.org/article/d63d7060e90a4429bad98c315860ed6b |
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ftdoajarticles:oai:doaj.org/article:d63d7060e90a4429bad98c315860ed6b 2023-09-05T13:11:34+02:00 DNA sequencing confirms meningeal worm (Parelaphostrongylus tenuis) and muscle worm (Parelaphostrongylus andersoni) in white-tailed deer (Odocoileus virginianus): Implications for moose (Alces alces) management Ashley J. Pidwerbesky Carly J. Gair Charlene N. Berkvens Trent K. Bollinger Jillian T. Detwiler 2023-08-01T00:00:00Z https://doi.org/10.1016/j.ijppaw.2023.01.009 https://doaj.org/article/d63d7060e90a4429bad98c315860ed6b EN eng Elsevier http://www.sciencedirect.com/science/article/pii/S2213224423000093 https://doaj.org/toc/2213-2244 2213-2244 doi:10.1016/j.ijppaw.2023.01.009 https://doaj.org/article/d63d7060e90a4429bad98c315860ed6b International Journal for Parasitology: Parasites and Wildlife, Vol 21, Iss , Pp 305-312 (2023) Alces alces Dorsal-spined larvae Genetic identification Protostrongylidae Transmission risk Wildlife health Zoology QL1-991 article 2023 ftdoajarticles https://doi.org/10.1016/j.ijppaw.2023.01.009 2023-08-13T00:40:56Z In North America, some moose populations are declining, and meningeal worm (Parelaphostrongylus tenuis) infections may be contributing. Moose are aberrant hosts for meningeal worm and develop severe pathology whereas white-tailed deer (WTD) are definitive hosts that experience minimal pathology and spread parasite larvae into the environment. Analyses of harvested WTD heads confirmed meningeal worm in Western Manitoba, Canada including in areas where moose have experienced population declines and are currently of management concern. The prevalence of larval meningeal worm from WTD feces in these areas are unknown, particularly because the dorsal-spined larvae (DSL) are morphologically indistinguishable from muscle worm (Parelaphostrongylus andersoni). To assess transmission risk of DSL, we investigated the spatial and temporal variation of prevalence in WTD feces from four areas (two with historical moose population declines and two without) sampled across two summers. We predicted higher prevalence of DSL in areas where moose are of management concern and surveys have shown higher meningeal worm prevalence in WTD heads. Further, we expected to only recover meningeal worm, as muscle worm has only been reported from caribou in more northern areas of Manitoba. We collected WTD feces by transect sampling, used the Baermann technique to obtain larvae, and sequenced partial cytochrome oxidase 1 and internal transcribed spacer 2 genes to confirm species identity. Zero-inflated models revealed that DSL prevalence did not differ temporally but was higher in areas where moose are of management concern. Genetic analyses revealed that meningeal worm and muscle worm were both present in Western Manitoba and co-occurred in three areas. Our results reveal novel insights into the geographic distribution of muscle worm and emphasize the importance of DNA sequencing for DSL identification. We suggest that concern for moose populations is warranted given the increased risk of parasite infection in some management areas. Article in Journal/Newspaper Alces alces Directory of Open Access Journals: DOAJ Articles Canada International Journal for Parasitology: Parasites and Wildlife 21 305 312 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Alces alces Dorsal-spined larvae Genetic identification Protostrongylidae Transmission risk Wildlife health Zoology QL1-991 |
spellingShingle |
Alces alces Dorsal-spined larvae Genetic identification Protostrongylidae Transmission risk Wildlife health Zoology QL1-991 Ashley J. Pidwerbesky Carly J. Gair Charlene N. Berkvens Trent K. Bollinger Jillian T. Detwiler DNA sequencing confirms meningeal worm (Parelaphostrongylus tenuis) and muscle worm (Parelaphostrongylus andersoni) in white-tailed deer (Odocoileus virginianus): Implications for moose (Alces alces) management |
topic_facet |
Alces alces Dorsal-spined larvae Genetic identification Protostrongylidae Transmission risk Wildlife health Zoology QL1-991 |
description |
In North America, some moose populations are declining, and meningeal worm (Parelaphostrongylus tenuis) infections may be contributing. Moose are aberrant hosts for meningeal worm and develop severe pathology whereas white-tailed deer (WTD) are definitive hosts that experience minimal pathology and spread parasite larvae into the environment. Analyses of harvested WTD heads confirmed meningeal worm in Western Manitoba, Canada including in areas where moose have experienced population declines and are currently of management concern. The prevalence of larval meningeal worm from WTD feces in these areas are unknown, particularly because the dorsal-spined larvae (DSL) are morphologically indistinguishable from muscle worm (Parelaphostrongylus andersoni). To assess transmission risk of DSL, we investigated the spatial and temporal variation of prevalence in WTD feces from four areas (two with historical moose population declines and two without) sampled across two summers. We predicted higher prevalence of DSL in areas where moose are of management concern and surveys have shown higher meningeal worm prevalence in WTD heads. Further, we expected to only recover meningeal worm, as muscle worm has only been reported from caribou in more northern areas of Manitoba. We collected WTD feces by transect sampling, used the Baermann technique to obtain larvae, and sequenced partial cytochrome oxidase 1 and internal transcribed spacer 2 genes to confirm species identity. Zero-inflated models revealed that DSL prevalence did not differ temporally but was higher in areas where moose are of management concern. Genetic analyses revealed that meningeal worm and muscle worm were both present in Western Manitoba and co-occurred in three areas. Our results reveal novel insights into the geographic distribution of muscle worm and emphasize the importance of DNA sequencing for DSL identification. We suggest that concern for moose populations is warranted given the increased risk of parasite infection in some management areas. |
format |
Article in Journal/Newspaper |
author |
Ashley J. Pidwerbesky Carly J. Gair Charlene N. Berkvens Trent K. Bollinger Jillian T. Detwiler |
author_facet |
Ashley J. Pidwerbesky Carly J. Gair Charlene N. Berkvens Trent K. Bollinger Jillian T. Detwiler |
author_sort |
Ashley J. Pidwerbesky |
title |
DNA sequencing confirms meningeal worm (Parelaphostrongylus tenuis) and muscle worm (Parelaphostrongylus andersoni) in white-tailed deer (Odocoileus virginianus): Implications for moose (Alces alces) management |
title_short |
DNA sequencing confirms meningeal worm (Parelaphostrongylus tenuis) and muscle worm (Parelaphostrongylus andersoni) in white-tailed deer (Odocoileus virginianus): Implications for moose (Alces alces) management |
title_full |
DNA sequencing confirms meningeal worm (Parelaphostrongylus tenuis) and muscle worm (Parelaphostrongylus andersoni) in white-tailed deer (Odocoileus virginianus): Implications for moose (Alces alces) management |
title_fullStr |
DNA sequencing confirms meningeal worm (Parelaphostrongylus tenuis) and muscle worm (Parelaphostrongylus andersoni) in white-tailed deer (Odocoileus virginianus): Implications for moose (Alces alces) management |
title_full_unstemmed |
DNA sequencing confirms meningeal worm (Parelaphostrongylus tenuis) and muscle worm (Parelaphostrongylus andersoni) in white-tailed deer (Odocoileus virginianus): Implications for moose (Alces alces) management |
title_sort |
dna sequencing confirms meningeal worm (parelaphostrongylus tenuis) and muscle worm (parelaphostrongylus andersoni) in white-tailed deer (odocoileus virginianus): implications for moose (alces alces) management |
publisher |
Elsevier |
publishDate |
2023 |
url |
https://doi.org/10.1016/j.ijppaw.2023.01.009 https://doaj.org/article/d63d7060e90a4429bad98c315860ed6b |
geographic |
Canada |
geographic_facet |
Canada |
genre |
Alces alces |
genre_facet |
Alces alces |
op_source |
International Journal for Parasitology: Parasites and Wildlife, Vol 21, Iss , Pp 305-312 (2023) |
op_relation |
http://www.sciencedirect.com/science/article/pii/S2213224423000093 https://doaj.org/toc/2213-2244 2213-2244 doi:10.1016/j.ijppaw.2023.01.009 https://doaj.org/article/d63d7060e90a4429bad98c315860ed6b |
op_doi |
https://doi.org/10.1016/j.ijppaw.2023.01.009 |
container_title |
International Journal for Parasitology: Parasites and Wildlife |
container_volume |
21 |
container_start_page |
305 |
op_container_end_page |
312 |
_version_ |
1776205131521458176 |