Dietary Cholesterol Differentially Regulates the Muscle Lipidomics of Farmed Turbot and Tiger Puffer

Exogenous cholesterol has been supplemented into aqua-feeds due to the reduced proportions of fishmeal and fish oil. This study aimed to investigate the effects of dietary cholesterol supplementation on the muscle lipidomics of two marine fish species, turbot and tiger puffer. A 70-day feeding trial...

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Bibliographic Details
Published in:Animals
Main Authors: Xiaoxue Meng, Qingzhu Bi, Qiang Ma, Yuliang Wei, Yanlu Li, Mengqing Liang, Houguo Xu
Format: Article in Journal/Newspaper
Language:English
Published: MDPI AG 2023
Subjects:
flatfish
pufferfish
cholesterol
muscle
lipidomics
Veterinary medicine
SF600-1100
Zoology
QL1-991
Turbot
Online Access:https://doi.org/10.3390/ani13101632
https://doaj.org/article/d62d02776d6b4d4bb4565271993da30b
Description
Summary:Exogenous cholesterol has been supplemented into aqua-feeds due to the reduced proportions of fishmeal and fish oil. This study aimed to investigate the effects of dietary cholesterol supplementation on the muscle lipidomics of two marine fish species, turbot and tiger puffer. A 70-day feeding trial was conducted, where two low-fishmeal diets supplemented with 0 or 1% cholesterol were used. The lipidomic analysis with targeted tandem mass spectrometry showed that, in turbot, a total of 49 individual lipids exhibited significant differences in their abundance in response to dietary cholesterol, whereas the number was 30 for tiger puffer. Dietary cholesterol up-regulated the abundance of cholesterol and cholesterol ester in both species. In turbot, the dietary cholesterol also increased the abundance of triacylglycerol and acylcarnitine, whereas in tiger puffer, it primarily regulated the abundance of phospholipids and BMP. This was the first time the responses of marine fish muscle lipidomics to dietary cholesterol supplementation have been investigated.

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