Immunostimulatory properties of dendritic cells after Leishmania donovani infection using an in vitro model of liver microenvironment.
BACKGROUND: Recent advances demonstrated that liver dendritic cells (DCs) promote immunologic hyporesponsiveness that may contribute to hepatic tolerance. Although there has been significant work on the phenotypic and functional roles of such DCs, the impact of liver microenvironment on the immune p...
Published in: | PLoS Neglected Tropical Diseases |
---|---|
Main Authors: | , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Public Library of Science (PLoS)
2010
|
Subjects: | |
Online Access: | https://doi.org/10.1371/journal.pntd.0000703 https://doaj.org/article/d6073ba4af774d28b05ed80e97b5bc87 |
id |
ftdoajarticles:oai:doaj.org/article:d6073ba4af774d28b05ed80e97b5bc87 |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:d6073ba4af774d28b05ed80e97b5bc87 2023-05-15T15:10:38+02:00 Immunostimulatory properties of dendritic cells after Leishmania donovani infection using an in vitro model of liver microenvironment. Ludovic Donaghy Florian Cabillic Anne Corlu Octavie Rostan Olivier Toutirais Christiane Guguen-Guillouzo Claude Guiguen Jean-Pierre Gangneux 2010-01-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0000703 https://doaj.org/article/d6073ba4af774d28b05ed80e97b5bc87 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC2882335?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0000703 https://doaj.org/article/d6073ba4af774d28b05ed80e97b5bc87 PLoS Neglected Tropical Diseases, Vol 4, Iss 6, p e703 (2010) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2010 ftdoajarticles https://doi.org/10.1371/journal.pntd.0000703 2022-12-30T23:28:03Z BACKGROUND: Recent advances demonstrated that liver dendritic cells (DCs) promote immunologic hyporesponsiveness that may contribute to hepatic tolerance. Although there has been significant work on the phenotypic and functional roles of such DCs, the impact of liver microenvironment on the immune properties of infected DC is still poorly explored, probably because of the limitations of modelization. METHODOLOGY/PRINCIPAL FINDINGS: Here, we hypothesized that DC tolerogenic properties have an impact on the antimicrobial response, particularly during the infection by the protozoan parasite Leishmania donovani. Indeed, a lymphocytic Th2 environment was reported to favour the growth and proliferation of L. donovani. We first modelized an adequate monocyte-differentiated DC model, either in rat liver epithelial cell- or in a human hepatic non-parenchymal cell-conditioned medium in order to infect them further. We established that DCs differentiated in a hepatic microenvironment displayed a CD14+/CD16+/CD123+ phenotype, secreted low IL-12p70 and had an impaired capacity to stimulate allogeneic T lymphocyte proliferation and IFNgamma secretion. We then infected DCs with L. donovani in the in vitro-defined hepatic microenvironment. The infection of hepatic DCs restored their capacity to stimulate allogeneic T-cell proliferation and to induce lymphocytic secretion of IFNgamma. Such characteristics were recently shown to favour granuloma formation in mice liver. CONCLUSIONS/SIGNIFICANCE: Our results suggest that the specific immunostimulatory properties of infected hepatic DCs might amplify the granuloma maturation, which warrants the effective control of infection in the liver during visceral leishmaniasis. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLoS Neglected Tropical Diseases 4 6 e703 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Ludovic Donaghy Florian Cabillic Anne Corlu Octavie Rostan Olivier Toutirais Christiane Guguen-Guillouzo Claude Guiguen Jean-Pierre Gangneux Immunostimulatory properties of dendritic cells after Leishmania donovani infection using an in vitro model of liver microenvironment. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
BACKGROUND: Recent advances demonstrated that liver dendritic cells (DCs) promote immunologic hyporesponsiveness that may contribute to hepatic tolerance. Although there has been significant work on the phenotypic and functional roles of such DCs, the impact of liver microenvironment on the immune properties of infected DC is still poorly explored, probably because of the limitations of modelization. METHODOLOGY/PRINCIPAL FINDINGS: Here, we hypothesized that DC tolerogenic properties have an impact on the antimicrobial response, particularly during the infection by the protozoan parasite Leishmania donovani. Indeed, a lymphocytic Th2 environment was reported to favour the growth and proliferation of L. donovani. We first modelized an adequate monocyte-differentiated DC model, either in rat liver epithelial cell- or in a human hepatic non-parenchymal cell-conditioned medium in order to infect them further. We established that DCs differentiated in a hepatic microenvironment displayed a CD14+/CD16+/CD123+ phenotype, secreted low IL-12p70 and had an impaired capacity to stimulate allogeneic T lymphocyte proliferation and IFNgamma secretion. We then infected DCs with L. donovani in the in vitro-defined hepatic microenvironment. The infection of hepatic DCs restored their capacity to stimulate allogeneic T-cell proliferation and to induce lymphocytic secretion of IFNgamma. Such characteristics were recently shown to favour granuloma formation in mice liver. CONCLUSIONS/SIGNIFICANCE: Our results suggest that the specific immunostimulatory properties of infected hepatic DCs might amplify the granuloma maturation, which warrants the effective control of infection in the liver during visceral leishmaniasis. |
format |
Article in Journal/Newspaper |
author |
Ludovic Donaghy Florian Cabillic Anne Corlu Octavie Rostan Olivier Toutirais Christiane Guguen-Guillouzo Claude Guiguen Jean-Pierre Gangneux |
author_facet |
Ludovic Donaghy Florian Cabillic Anne Corlu Octavie Rostan Olivier Toutirais Christiane Guguen-Guillouzo Claude Guiguen Jean-Pierre Gangneux |
author_sort |
Ludovic Donaghy |
title |
Immunostimulatory properties of dendritic cells after Leishmania donovani infection using an in vitro model of liver microenvironment. |
title_short |
Immunostimulatory properties of dendritic cells after Leishmania donovani infection using an in vitro model of liver microenvironment. |
title_full |
Immunostimulatory properties of dendritic cells after Leishmania donovani infection using an in vitro model of liver microenvironment. |
title_fullStr |
Immunostimulatory properties of dendritic cells after Leishmania donovani infection using an in vitro model of liver microenvironment. |
title_full_unstemmed |
Immunostimulatory properties of dendritic cells after Leishmania donovani infection using an in vitro model of liver microenvironment. |
title_sort |
immunostimulatory properties of dendritic cells after leishmania donovani infection using an in vitro model of liver microenvironment. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2010 |
url |
https://doi.org/10.1371/journal.pntd.0000703 https://doaj.org/article/d6073ba4af774d28b05ed80e97b5bc87 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 4, Iss 6, p e703 (2010) |
op_relation |
http://europepmc.org/articles/PMC2882335?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0000703 https://doaj.org/article/d6073ba4af774d28b05ed80e97b5bc87 |
op_doi |
https://doi.org/10.1371/journal.pntd.0000703 |
container_title |
PLoS Neglected Tropical Diseases |
container_volume |
4 |
container_issue |
6 |
container_start_page |
e703 |
_version_ |
1766341622252961792 |