Temperature and pH Profiling of Extracellular Amylase from Antarctic and Arctic Soil Microfungi
While diversity studies and screening for enzyme activities are important elements of understanding fungal roles in the soil ecosystem, extracting and purifying the target enzyme from the fungal cellular system is also required to characterize the enzyme. This is, in particular, necessary before dev...
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Online Access: | https://doi.org/10.3390/fermentation8110601 https://doaj.org/article/d5daf09549274f74bc905e58ba5e8f3a |
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ftdoajarticles:oai:doaj.org/article:d5daf09549274f74bc905e58ba5e8f3a 2023-05-15T13:55:26+02:00 Temperature and pH Profiling of Extracellular Amylase from Antarctic and Arctic Soil Microfungi Abiramy Krishnan Zazali Alias Peter Convey Marcelo González-Aravena Jerzy Smykla Mohammed Rizman-Idid Siti Aisyah Alias 2022-11-01T00:00:00Z https://doi.org/10.3390/fermentation8110601 https://doaj.org/article/d5daf09549274f74bc905e58ba5e8f3a EN eng MDPI AG https://www.mdpi.com/2311-5637/8/11/601 https://doaj.org/toc/2311-5637 doi:10.3390/fermentation8110601 2311-5637 https://doaj.org/article/d5daf09549274f74bc905e58ba5e8f3a Fermentation, Vol 8, Iss 601, p 601 (2022) α-amylase enzyme Antarctic Arctic temperature pH Fermentation industries. Beverages. Alcohol TP500-660 article 2022 ftdoajarticles https://doi.org/10.3390/fermentation8110601 2022-12-30T20:09:18Z While diversity studies and screening for enzyme activities are important elements of understanding fungal roles in the soil ecosystem, extracting and purifying the target enzyme from the fungal cellular system is also required to characterize the enzyme. This is, in particular, necessary before developing the enzyme for industrial-scale production. In the present study, partially purified α-amylase was obtained from strains of Pseudogymnoascus sp. obtained from Antarctic and Arctic locations. Partially purified α-amylases from these polar fungi exhibited very similar characteristics, including being active at 15 °C, although having a small difference in optimum pH. Both fungal taxa are good candidates for the potential application of cold-active enzymes in biotechnological industries, and further purification and characterization steps are now required. The α-amylases from polar fungi are attractive in terms of industrial development because they are active at lower temperatures and acidic pH, thus potentially creating energy and cost savings. Furthermore, they prevent the production of maltulose, which is an undesirable by-product often formed under alkaline conditions. Psychrophilic amylases from the polar Pseudogymnoascus sp. investigated in the present study could provide a valuable future contribution to biotechnological applications. Article in Journal/Newspaper Antarc* Antarctic Arctic Directory of Open Access Journals: DOAJ Articles Arctic Antarctic Fermentation 8 11 601 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
α-amylase enzyme Antarctic Arctic temperature pH Fermentation industries. Beverages. Alcohol TP500-660 |
spellingShingle |
α-amylase enzyme Antarctic Arctic temperature pH Fermentation industries. Beverages. Alcohol TP500-660 Abiramy Krishnan Zazali Alias Peter Convey Marcelo González-Aravena Jerzy Smykla Mohammed Rizman-Idid Siti Aisyah Alias Temperature and pH Profiling of Extracellular Amylase from Antarctic and Arctic Soil Microfungi |
topic_facet |
α-amylase enzyme Antarctic Arctic temperature pH Fermentation industries. Beverages. Alcohol TP500-660 |
description |
While diversity studies and screening for enzyme activities are important elements of understanding fungal roles in the soil ecosystem, extracting and purifying the target enzyme from the fungal cellular system is also required to characterize the enzyme. This is, in particular, necessary before developing the enzyme for industrial-scale production. In the present study, partially purified α-amylase was obtained from strains of Pseudogymnoascus sp. obtained from Antarctic and Arctic locations. Partially purified α-amylases from these polar fungi exhibited very similar characteristics, including being active at 15 °C, although having a small difference in optimum pH. Both fungal taxa are good candidates for the potential application of cold-active enzymes in biotechnological industries, and further purification and characterization steps are now required. The α-amylases from polar fungi are attractive in terms of industrial development because they are active at lower temperatures and acidic pH, thus potentially creating energy and cost savings. Furthermore, they prevent the production of maltulose, which is an undesirable by-product often formed under alkaline conditions. Psychrophilic amylases from the polar Pseudogymnoascus sp. investigated in the present study could provide a valuable future contribution to biotechnological applications. |
format |
Article in Journal/Newspaper |
author |
Abiramy Krishnan Zazali Alias Peter Convey Marcelo González-Aravena Jerzy Smykla Mohammed Rizman-Idid Siti Aisyah Alias |
author_facet |
Abiramy Krishnan Zazali Alias Peter Convey Marcelo González-Aravena Jerzy Smykla Mohammed Rizman-Idid Siti Aisyah Alias |
author_sort |
Abiramy Krishnan |
title |
Temperature and pH Profiling of Extracellular Amylase from Antarctic and Arctic Soil Microfungi |
title_short |
Temperature and pH Profiling of Extracellular Amylase from Antarctic and Arctic Soil Microfungi |
title_full |
Temperature and pH Profiling of Extracellular Amylase from Antarctic and Arctic Soil Microfungi |
title_fullStr |
Temperature and pH Profiling of Extracellular Amylase from Antarctic and Arctic Soil Microfungi |
title_full_unstemmed |
Temperature and pH Profiling of Extracellular Amylase from Antarctic and Arctic Soil Microfungi |
title_sort |
temperature and ph profiling of extracellular amylase from antarctic and arctic soil microfungi |
publisher |
MDPI AG |
publishDate |
2022 |
url |
https://doi.org/10.3390/fermentation8110601 https://doaj.org/article/d5daf09549274f74bc905e58ba5e8f3a |
geographic |
Arctic Antarctic |
geographic_facet |
Arctic Antarctic |
genre |
Antarc* Antarctic Arctic |
genre_facet |
Antarc* Antarctic Arctic |
op_source |
Fermentation, Vol 8, Iss 601, p 601 (2022) |
op_relation |
https://www.mdpi.com/2311-5637/8/11/601 https://doaj.org/toc/2311-5637 doi:10.3390/fermentation8110601 2311-5637 https://doaj.org/article/d5daf09549274f74bc905e58ba5e8f3a |
op_doi |
https://doi.org/10.3390/fermentation8110601 |
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Fermentation |
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8 |
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11 |
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601 |
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1766262052674863104 |