Water, water everywhere: environmental DNA can unlock population structure in elusive marine species
Determining management units for natural populations is critical for effective conservation and management. However, collecting the requisite tissue samples for population genetic analyses remains the primary limiting factor for a number of marine species. The harbour porpoise (Phocoena phocoena), o...
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ftdoajarticles:oai:doaj.org/article:d5d22749223946c59068866fdbb9fd2d 2023-05-15T16:33:25+02:00 Water, water everywhere: environmental DNA can unlock population structure in elusive marine species Kim M. Parsons Meredith Everett Marilyn Dahlheim Linda Park 2018-01-01T00:00:00Z https://doi.org/10.1098/rsos.180537 https://doaj.org/article/d5d22749223946c59068866fdbb9fd2d EN eng The Royal Society https://royalsocietypublishing.org/doi/pdf/10.1098/rsos.180537 https://doaj.org/toc/2054-5703 2054-5703 doi:10.1098/rsos.180537 https://doaj.org/article/d5d22749223946c59068866fdbb9fd2d Royal Society Open Science, Vol 5, Iss 8 (2018) porpoise environmental dna population genetics cetacean stock next-generation sequencing Science Q article 2018 ftdoajarticles https://doi.org/10.1098/rsos.180537 2022-12-31T04:17:28Z Determining management units for natural populations is critical for effective conservation and management. However, collecting the requisite tissue samples for population genetic analyses remains the primary limiting factor for a number of marine species. The harbour porpoise (Phocoena phocoena), one of the smallest cetaceans in the Northern Hemisphere, is a primary example. These elusive, highly mobile small animals confound traditional approaches of collecting tissue samples for genetic analyses, yet their nearshore habitat makes them highly vulnerable to fisheries by-catch and the effects of habitat degradation. By exploiting the naturally shed cellular material in seawater and the power of next-generation sequencing, we develop a novel approach for generating population-specific mitochondrial sequence data from environmental DNA (eDNA) using surface seawater samples. Indications of significant genetic differentiation within a currently recognized management stock highlights the need for dedicated eDNA sampling throughout the population's range in southeast Alaska. This indirect sampling tactic for characterizing stock structure of small and endangered marine mammals has the potential to revolutionize population assessment for otherwise inaccessible marine taxa. Article in Journal/Newspaper Harbour porpoise Phocoena phocoena Alaska Directory of Open Access Journals: DOAJ Articles Royal Society Open Science 5 8 180537 |
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Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
porpoise environmental dna population genetics cetacean stock next-generation sequencing Science Q |
spellingShingle |
porpoise environmental dna population genetics cetacean stock next-generation sequencing Science Q Kim M. Parsons Meredith Everett Marilyn Dahlheim Linda Park Water, water everywhere: environmental DNA can unlock population structure in elusive marine species |
topic_facet |
porpoise environmental dna population genetics cetacean stock next-generation sequencing Science Q |
description |
Determining management units for natural populations is critical for effective conservation and management. However, collecting the requisite tissue samples for population genetic analyses remains the primary limiting factor for a number of marine species. The harbour porpoise (Phocoena phocoena), one of the smallest cetaceans in the Northern Hemisphere, is a primary example. These elusive, highly mobile small animals confound traditional approaches of collecting tissue samples for genetic analyses, yet their nearshore habitat makes them highly vulnerable to fisheries by-catch and the effects of habitat degradation. By exploiting the naturally shed cellular material in seawater and the power of next-generation sequencing, we develop a novel approach for generating population-specific mitochondrial sequence data from environmental DNA (eDNA) using surface seawater samples. Indications of significant genetic differentiation within a currently recognized management stock highlights the need for dedicated eDNA sampling throughout the population's range in southeast Alaska. This indirect sampling tactic for characterizing stock structure of small and endangered marine mammals has the potential to revolutionize population assessment for otherwise inaccessible marine taxa. |
format |
Article in Journal/Newspaper |
author |
Kim M. Parsons Meredith Everett Marilyn Dahlheim Linda Park |
author_facet |
Kim M. Parsons Meredith Everett Marilyn Dahlheim Linda Park |
author_sort |
Kim M. Parsons |
title |
Water, water everywhere: environmental DNA can unlock population structure in elusive marine species |
title_short |
Water, water everywhere: environmental DNA can unlock population structure in elusive marine species |
title_full |
Water, water everywhere: environmental DNA can unlock population structure in elusive marine species |
title_fullStr |
Water, water everywhere: environmental DNA can unlock population structure in elusive marine species |
title_full_unstemmed |
Water, water everywhere: environmental DNA can unlock population structure in elusive marine species |
title_sort |
water, water everywhere: environmental dna can unlock population structure in elusive marine species |
publisher |
The Royal Society |
publishDate |
2018 |
url |
https://doi.org/10.1098/rsos.180537 https://doaj.org/article/d5d22749223946c59068866fdbb9fd2d |
genre |
Harbour porpoise Phocoena phocoena Alaska |
genre_facet |
Harbour porpoise Phocoena phocoena Alaska |
op_source |
Royal Society Open Science, Vol 5, Iss 8 (2018) |
op_relation |
https://royalsocietypublishing.org/doi/pdf/10.1098/rsos.180537 https://doaj.org/toc/2054-5703 2054-5703 doi:10.1098/rsos.180537 https://doaj.org/article/d5d22749223946c59068866fdbb9fd2d |
op_doi |
https://doi.org/10.1098/rsos.180537 |
container_title |
Royal Society Open Science |
container_volume |
5 |
container_issue |
8 |
container_start_page |
180537 |
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1766023108651646976 |