Dynamic motions of ice-binding proteins in living Caenorhabditis elegans using diffracted X-ray blinking and tracking
The dynamic properties of protein molecules are involved in the relationship between their structure and function. Time-resolved X-ray observation enables capturing the structures of biomolecules with picometre-scale precision. However, this technique has yet to be implemented in living animals. Her...
Published in: | Biochemistry and Biophysics Reports |
---|---|
Main Authors: | , , , , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Elsevier
2022
|
Subjects: | |
Online Access: | https://doi.org/10.1016/j.bbrep.2022.101224 https://doaj.org/article/d51b7b0d33ea4dc7a01a263f0631b0b5 |
id |
ftdoajarticles:oai:doaj.org/article:d51b7b0d33ea4dc7a01a263f0631b0b5 |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:d51b7b0d33ea4dc7a01a263f0631b0b5 2023-05-15T13:44:31+02:00 Dynamic motions of ice-binding proteins in living Caenorhabditis elegans using diffracted X-ray blinking and tracking Masahiro Kuramochi Yige Dong Yue Yang Tatsuya Arai Rio Okada Yoichi Shinkai Motomichi Doi Kouki Aoyama Hiroshi Sekiguchi Kazuhiro Mio Sakae Tsuda Yuji C. Sasaki 2022-03-01T00:00:00Z https://doi.org/10.1016/j.bbrep.2022.101224 https://doaj.org/article/d51b7b0d33ea4dc7a01a263f0631b0b5 EN eng Elsevier http://www.sciencedirect.com/science/article/pii/S2405580822000255 https://doaj.org/toc/2405-5808 2405-5808 doi:10.1016/j.bbrep.2022.101224 https://doaj.org/article/d51b7b0d33ea4dc7a01a263f0631b0b5 Biochemistry and Biophysics Reports, Vol 29, Iss , Pp 101224- (2022) Diffracted X-ray tracking (DXT) Diffracted X-ray blinking (DXB) C. elegans Ice-binding protein Biology (General) QH301-705.5 Biochemistry QD415-436 article 2022 ftdoajarticles https://doi.org/10.1016/j.bbrep.2022.101224 2022-12-31T10:03:19Z The dynamic properties of protein molecules are involved in the relationship between their structure and function. Time-resolved X-ray observation enables capturing the structures of biomolecules with picometre-scale precision. However, this technique has yet to be implemented in living animals. Here, we examined diffracted X-ray blinking (DXB) and diffracted X-ray tracking (DXT) to observe the dynamics of a protein located on intestinal cells in adult Caenorhabditis elegans. This in vivo tissue-specific DXB was examined at temperatures from 20 °C to −10 °C for a recombinant ice-binding protein from Antarctomyces psychrotrophicus (AnpIBP) connected with the cells through a transmembrane CD4 protein equipped with a glycine-serine linker. AnpIBP inhibits ice growth at subzero temperatures by binding to ice crystals. We found that the rotational motion of AnpIBP decreases at −10 °C. In contrast, the motion of the AnpIBP mutant, which has a defective ice-binding ability, did not decrease at −10 °C. The twisting and tilting motional speeds of AnpIBPs measured above 5 °C by DXT were always higher than those of the defective AnpIBP mutant. These results suggest that wild-type AnpIBP is highly mobile in solution, and it is halted at subzero temperatures through ice binding. DXB and DXT allow for exploring protein behaviour in live animals with subnano resolution precision. Article in Journal/Newspaper Antarc* Directory of Open Access Journals: DOAJ Articles Tilting ENVELOPE(-54.065,-54.065,49.700,49.700) Biochemistry and Biophysics Reports 29 101224 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Diffracted X-ray tracking (DXT) Diffracted X-ray blinking (DXB) C. elegans Ice-binding protein Biology (General) QH301-705.5 Biochemistry QD415-436 |
spellingShingle |
Diffracted X-ray tracking (DXT) Diffracted X-ray blinking (DXB) C. elegans Ice-binding protein Biology (General) QH301-705.5 Biochemistry QD415-436 Masahiro Kuramochi Yige Dong Yue Yang Tatsuya Arai Rio Okada Yoichi Shinkai Motomichi Doi Kouki Aoyama Hiroshi Sekiguchi Kazuhiro Mio Sakae Tsuda Yuji C. Sasaki Dynamic motions of ice-binding proteins in living Caenorhabditis elegans using diffracted X-ray blinking and tracking |
topic_facet |
Diffracted X-ray tracking (DXT) Diffracted X-ray blinking (DXB) C. elegans Ice-binding protein Biology (General) QH301-705.5 Biochemistry QD415-436 |
description |
The dynamic properties of protein molecules are involved in the relationship between their structure and function. Time-resolved X-ray observation enables capturing the structures of biomolecules with picometre-scale precision. However, this technique has yet to be implemented in living animals. Here, we examined diffracted X-ray blinking (DXB) and diffracted X-ray tracking (DXT) to observe the dynamics of a protein located on intestinal cells in adult Caenorhabditis elegans. This in vivo tissue-specific DXB was examined at temperatures from 20 °C to −10 °C for a recombinant ice-binding protein from Antarctomyces psychrotrophicus (AnpIBP) connected with the cells through a transmembrane CD4 protein equipped with a glycine-serine linker. AnpIBP inhibits ice growth at subzero temperatures by binding to ice crystals. We found that the rotational motion of AnpIBP decreases at −10 °C. In contrast, the motion of the AnpIBP mutant, which has a defective ice-binding ability, did not decrease at −10 °C. The twisting and tilting motional speeds of AnpIBPs measured above 5 °C by DXT were always higher than those of the defective AnpIBP mutant. These results suggest that wild-type AnpIBP is highly mobile in solution, and it is halted at subzero temperatures through ice binding. DXB and DXT allow for exploring protein behaviour in live animals with subnano resolution precision. |
format |
Article in Journal/Newspaper |
author |
Masahiro Kuramochi Yige Dong Yue Yang Tatsuya Arai Rio Okada Yoichi Shinkai Motomichi Doi Kouki Aoyama Hiroshi Sekiguchi Kazuhiro Mio Sakae Tsuda Yuji C. Sasaki |
author_facet |
Masahiro Kuramochi Yige Dong Yue Yang Tatsuya Arai Rio Okada Yoichi Shinkai Motomichi Doi Kouki Aoyama Hiroshi Sekiguchi Kazuhiro Mio Sakae Tsuda Yuji C. Sasaki |
author_sort |
Masahiro Kuramochi |
title |
Dynamic motions of ice-binding proteins in living Caenorhabditis elegans using diffracted X-ray blinking and tracking |
title_short |
Dynamic motions of ice-binding proteins in living Caenorhabditis elegans using diffracted X-ray blinking and tracking |
title_full |
Dynamic motions of ice-binding proteins in living Caenorhabditis elegans using diffracted X-ray blinking and tracking |
title_fullStr |
Dynamic motions of ice-binding proteins in living Caenorhabditis elegans using diffracted X-ray blinking and tracking |
title_full_unstemmed |
Dynamic motions of ice-binding proteins in living Caenorhabditis elegans using diffracted X-ray blinking and tracking |
title_sort |
dynamic motions of ice-binding proteins in living caenorhabditis elegans using diffracted x-ray blinking and tracking |
publisher |
Elsevier |
publishDate |
2022 |
url |
https://doi.org/10.1016/j.bbrep.2022.101224 https://doaj.org/article/d51b7b0d33ea4dc7a01a263f0631b0b5 |
long_lat |
ENVELOPE(-54.065,-54.065,49.700,49.700) |
geographic |
Tilting |
geographic_facet |
Tilting |
genre |
Antarc* |
genre_facet |
Antarc* |
op_source |
Biochemistry and Biophysics Reports, Vol 29, Iss , Pp 101224- (2022) |
op_relation |
http://www.sciencedirect.com/science/article/pii/S2405580822000255 https://doaj.org/toc/2405-5808 2405-5808 doi:10.1016/j.bbrep.2022.101224 https://doaj.org/article/d51b7b0d33ea4dc7a01a263f0631b0b5 |
op_doi |
https://doi.org/10.1016/j.bbrep.2022.101224 |
container_title |
Biochemistry and Biophysics Reports |
container_volume |
29 |
container_start_page |
101224 |
_version_ |
1766202782673534976 |