IrSPI, a tick serine protease inhibitor involved in tick feeding and Bartonella henselae infection.
Ixodes ricinus is the most widespread and abundant tick in Europe, frequently bites humans, and is the vector of several pathogens including those responsible for Lyme disease, Tick-Borne Encephalitis, anaplasmosis, babesiosis and bartonellosis. These tick-borne pathogens are transmitted to vertebra...
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ftdoajarticles:oai:doaj.org/article:d514b2050b0f4b73bd0ffb5bfa935906 2023-05-15T15:14:05+02:00 IrSPI, a tick serine protease inhibitor involved in tick feeding and Bartonella henselae infection. Xiang Ye Liu Jose de la Fuente Martine Cote Ruth C Galindo Sara Moutailler Muriel Vayssier-Taussat Sarah I Bonnet 2014-07-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0002993 https://doaj.org/article/d514b2050b0f4b73bd0ffb5bfa935906 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC4109860?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0002993 https://doaj.org/article/d514b2050b0f4b73bd0ffb5bfa935906 PLoS Neglected Tropical Diseases, Vol 8, Iss 7, p e2993 (2014) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2014 ftdoajarticles https://doi.org/10.1371/journal.pntd.0002993 2022-12-31T03:49:14Z Ixodes ricinus is the most widespread and abundant tick in Europe, frequently bites humans, and is the vector of several pathogens including those responsible for Lyme disease, Tick-Borne Encephalitis, anaplasmosis, babesiosis and bartonellosis. These tick-borne pathogens are transmitted to vertebrate hosts via tick saliva during blood feeding, and tick salivary gland (SG) factors are likely implicated in transmission. In order to identify such tick factors, we characterized the transcriptome of female I. ricinus SGs using next generation sequencing techniques, and compared transcriptomes between Bartonella henselae-infected and non-infected ticks. High-throughput sequencing of I. ricinus SG transcriptomes led to the generation of 24,539 isotigs. Among them, 829 and 517 transcripts were either significantly up- or down-regulated respectively, in response to bacterial infection. Searches based on sequence identity showed that among the differentially expressed transcripts, 161 transcripts corresponded to nine groups of previously annotated tick SG gene families, while the others corresponded to genes of unknown function. Expression patterns of five selected genes belonging to the BPTI/Kunitz family of serine protease inhibitors, the tick salivary peptide group 1 protein, the salp15 super-family, and the arthropod defensin family, were validated by qRT-PCR. IrSPI, a member of the BPTI/Kunitz family of serine protease inhibitors, showed the highest up-regulation in SGs in response to Bartonella infection. IrSPI silencing impaired tick feeding, as well as resulted in reduced bacterial load in tick SGs. This study provides a comprehensive analysis of I. ricinus SG transcriptome and contributes significant genomic information about this important disease vector. This in-depth knowledge will enable a better understanding of the molecular interactions between ticks and tick-borne pathogens, and identifies IrSPI, a candidate to study now in detail to estimate its potentialities as vaccine against the ticks and the ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLoS Neglected Tropical Diseases 8 7 e2993 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
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language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Xiang Ye Liu Jose de la Fuente Martine Cote Ruth C Galindo Sara Moutailler Muriel Vayssier-Taussat Sarah I Bonnet IrSPI, a tick serine protease inhibitor involved in tick feeding and Bartonella henselae infection. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Ixodes ricinus is the most widespread and abundant tick in Europe, frequently bites humans, and is the vector of several pathogens including those responsible for Lyme disease, Tick-Borne Encephalitis, anaplasmosis, babesiosis and bartonellosis. These tick-borne pathogens are transmitted to vertebrate hosts via tick saliva during blood feeding, and tick salivary gland (SG) factors are likely implicated in transmission. In order to identify such tick factors, we characterized the transcriptome of female I. ricinus SGs using next generation sequencing techniques, and compared transcriptomes between Bartonella henselae-infected and non-infected ticks. High-throughput sequencing of I. ricinus SG transcriptomes led to the generation of 24,539 isotigs. Among them, 829 and 517 transcripts were either significantly up- or down-regulated respectively, in response to bacterial infection. Searches based on sequence identity showed that among the differentially expressed transcripts, 161 transcripts corresponded to nine groups of previously annotated tick SG gene families, while the others corresponded to genes of unknown function. Expression patterns of five selected genes belonging to the BPTI/Kunitz family of serine protease inhibitors, the tick salivary peptide group 1 protein, the salp15 super-family, and the arthropod defensin family, were validated by qRT-PCR. IrSPI, a member of the BPTI/Kunitz family of serine protease inhibitors, showed the highest up-regulation in SGs in response to Bartonella infection. IrSPI silencing impaired tick feeding, as well as resulted in reduced bacterial load in tick SGs. This study provides a comprehensive analysis of I. ricinus SG transcriptome and contributes significant genomic information about this important disease vector. This in-depth knowledge will enable a better understanding of the molecular interactions between ticks and tick-borne pathogens, and identifies IrSPI, a candidate to study now in detail to estimate its potentialities as vaccine against the ticks and the ... |
format |
Article in Journal/Newspaper |
author |
Xiang Ye Liu Jose de la Fuente Martine Cote Ruth C Galindo Sara Moutailler Muriel Vayssier-Taussat Sarah I Bonnet |
author_facet |
Xiang Ye Liu Jose de la Fuente Martine Cote Ruth C Galindo Sara Moutailler Muriel Vayssier-Taussat Sarah I Bonnet |
author_sort |
Xiang Ye Liu |
title |
IrSPI, a tick serine protease inhibitor involved in tick feeding and Bartonella henselae infection. |
title_short |
IrSPI, a tick serine protease inhibitor involved in tick feeding and Bartonella henselae infection. |
title_full |
IrSPI, a tick serine protease inhibitor involved in tick feeding and Bartonella henselae infection. |
title_fullStr |
IrSPI, a tick serine protease inhibitor involved in tick feeding and Bartonella henselae infection. |
title_full_unstemmed |
IrSPI, a tick serine protease inhibitor involved in tick feeding and Bartonella henselae infection. |
title_sort |
irspi, a tick serine protease inhibitor involved in tick feeding and bartonella henselae infection. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2014 |
url |
https://doi.org/10.1371/journal.pntd.0002993 https://doaj.org/article/d514b2050b0f4b73bd0ffb5bfa935906 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 8, Iss 7, p e2993 (2014) |
op_relation |
http://europepmc.org/articles/PMC4109860?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0002993 https://doaj.org/article/d514b2050b0f4b73bd0ffb5bfa935906 |
op_doi |
https://doi.org/10.1371/journal.pntd.0002993 |
container_title |
PLoS Neglected Tropical Diseases |
container_volume |
8 |
container_issue |
7 |
container_start_page |
e2993 |
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1766344584365867008 |