Delayed repair of the facial nerve and its negative impacts on nerve and muscle regeneration
Abstract Background: In this experimental protocol, we evaluated the immediate and delayed repair of the buccal branch of the facial nerve (BBFN) with heterologous fibrin biopolymer (HFB) as a coaptation medium and the use of photobiomodulation (PBM), performing functional and histomorphometric anal...
Published in: | Journal of Venomous Animals and Toxins including Tropical Diseases |
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Online Access: | https://doi.org/10.1590/1678-9199-jvatitd-2023-0093 https://doaj.org/article/d49e483323b742a9a75f0065466f47d0 |
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ftdoajarticles:oai:doaj.org/article:d49e483323b742a9a75f0065466f47d0 2024-09-09T19:28:07+00:00 Delayed repair of the facial nerve and its negative impacts on nerve and muscle regeneration Cleuber Rodrigo de Souza Bueno Daniela Vieira Buchaim Benedito Barraviera Rui Seabra Ferreira Jr. Paulo Sérgio da Silva Santos Carlos Henrique Bertoni Reis Marcelo Augusto Cini Milton Carlos Kuga Geraldo Marco Rosa Junior Rogerio Leone Buchaim 2024-05-01T00:00:00Z https://doi.org/10.1590/1678-9199-jvatitd-2023-0093 https://doaj.org/article/d49e483323b742a9a75f0065466f47d0 EN eng SciELO http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992024000100307&lng=en&tlng=en http://www.scielo.br/pdf/jvatitd/v30/1678-9199-jvatitd-30-e20230093.pdf https://doaj.org/toc/1678-9199 1678-9199 doi:10.1590/1678-9199-jvatitd-2023-0093 https://doaj.org/article/d49e483323b742a9a75f0065466f47d0 Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 30 (2024) Facial nerve Nerve regeneration Photobiomodulation Fibrin sealant Biopolymers Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 article 2024 ftdoajarticles https://doi.org/10.1590/1678-9199-jvatitd-2023-0093 2024-08-05T17:49:19Z Abstract Background: In this experimental protocol, we evaluated the immediate and delayed repair of the buccal branch of the facial nerve (BBFN) with heterologous fibrin biopolymer (HFB) as a coaptation medium and the use of photobiomodulation (PBM), performing functional and histomorphometric analysis of the BBFN and perioral muscles. Methods: Twenty-eight rats were divided into eight groups using the BBFN bilaterally (the left nerve was used for PBM), namely: G1 - control group, right BBFN (without injury); G2 - control group, left BBFN (without injury + PBM); G3 - Denervated right BBFN (neurotmesis); G4 - Denervated left BBFN (neurotmesis + PBM); G5 - Immediate repair of right BBFN (neurotmesis + HFB); G6 - Immediate repair of left BBFN (neurotmesis + HFB + PBM); G7 - Delayed repair of right BBFN (neurotmesis + HFB); G8 - Delayed repair of left BBFN (neurotmesis + HFB + PBM). Delayed repair occurred after two weeks of denervation. All animals were sacrificed after six weeks postoperatively. Results: In the parameters of the BBFN, we observed inferior results in the groups with delayed repair, in relation to the groups with immediate repair, with a significant difference (p < 0.05) in the diameter of the nerve fiber, the axon, and the thickness of the myelin sheath of the group with immediate repair with PBM compared to the other experimental groups. In measuring the muscle fiber area, groups G7 (826.4 ± 69.90) and G8 (836.7 ± 96.44) were similar to G5 (882.8 ± 70.51). In the functional analysis, the G7 (4.10 ± 0.07) and G8 (4.12 ± 0.08) groups presented normal parameters. Conclusion: We demonstrated that delayed repair of BBFN is possible with HFB, but with worse results compared to immediate repair, and that PBM has a positive influence on nerve regeneration results in immediate repair. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Journal of Venomous Animals and Toxins including Tropical Diseases 30 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Facial nerve Nerve regeneration Photobiomodulation Fibrin sealant Biopolymers Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
spellingShingle |
Facial nerve Nerve regeneration Photobiomodulation Fibrin sealant Biopolymers Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 Cleuber Rodrigo de Souza Bueno Daniela Vieira Buchaim Benedito Barraviera Rui Seabra Ferreira Jr. Paulo Sérgio da Silva Santos Carlos Henrique Bertoni Reis Marcelo Augusto Cini Milton Carlos Kuga Geraldo Marco Rosa Junior Rogerio Leone Buchaim Delayed repair of the facial nerve and its negative impacts on nerve and muscle regeneration |
topic_facet |
Facial nerve Nerve regeneration Photobiomodulation Fibrin sealant Biopolymers Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
description |
Abstract Background: In this experimental protocol, we evaluated the immediate and delayed repair of the buccal branch of the facial nerve (BBFN) with heterologous fibrin biopolymer (HFB) as a coaptation medium and the use of photobiomodulation (PBM), performing functional and histomorphometric analysis of the BBFN and perioral muscles. Methods: Twenty-eight rats were divided into eight groups using the BBFN bilaterally (the left nerve was used for PBM), namely: G1 - control group, right BBFN (without injury); G2 - control group, left BBFN (without injury + PBM); G3 - Denervated right BBFN (neurotmesis); G4 - Denervated left BBFN (neurotmesis + PBM); G5 - Immediate repair of right BBFN (neurotmesis + HFB); G6 - Immediate repair of left BBFN (neurotmesis + HFB + PBM); G7 - Delayed repair of right BBFN (neurotmesis + HFB); G8 - Delayed repair of left BBFN (neurotmesis + HFB + PBM). Delayed repair occurred after two weeks of denervation. All animals were sacrificed after six weeks postoperatively. Results: In the parameters of the BBFN, we observed inferior results in the groups with delayed repair, in relation to the groups with immediate repair, with a significant difference (p < 0.05) in the diameter of the nerve fiber, the axon, and the thickness of the myelin sheath of the group with immediate repair with PBM compared to the other experimental groups. In measuring the muscle fiber area, groups G7 (826.4 ± 69.90) and G8 (836.7 ± 96.44) were similar to G5 (882.8 ± 70.51). In the functional analysis, the G7 (4.10 ± 0.07) and G8 (4.12 ± 0.08) groups presented normal parameters. Conclusion: We demonstrated that delayed repair of BBFN is possible with HFB, but with worse results compared to immediate repair, and that PBM has a positive influence on nerve regeneration results in immediate repair. |
format |
Article in Journal/Newspaper |
author |
Cleuber Rodrigo de Souza Bueno Daniela Vieira Buchaim Benedito Barraviera Rui Seabra Ferreira Jr. Paulo Sérgio da Silva Santos Carlos Henrique Bertoni Reis Marcelo Augusto Cini Milton Carlos Kuga Geraldo Marco Rosa Junior Rogerio Leone Buchaim |
author_facet |
Cleuber Rodrigo de Souza Bueno Daniela Vieira Buchaim Benedito Barraviera Rui Seabra Ferreira Jr. Paulo Sérgio da Silva Santos Carlos Henrique Bertoni Reis Marcelo Augusto Cini Milton Carlos Kuga Geraldo Marco Rosa Junior Rogerio Leone Buchaim |
author_sort |
Cleuber Rodrigo de Souza Bueno |
title |
Delayed repair of the facial nerve and its negative impacts on nerve and muscle regeneration |
title_short |
Delayed repair of the facial nerve and its negative impacts on nerve and muscle regeneration |
title_full |
Delayed repair of the facial nerve and its negative impacts on nerve and muscle regeneration |
title_fullStr |
Delayed repair of the facial nerve and its negative impacts on nerve and muscle regeneration |
title_full_unstemmed |
Delayed repair of the facial nerve and its negative impacts on nerve and muscle regeneration |
title_sort |
delayed repair of the facial nerve and its negative impacts on nerve and muscle regeneration |
publisher |
SciELO |
publishDate |
2024 |
url |
https://doi.org/10.1590/1678-9199-jvatitd-2023-0093 https://doaj.org/article/d49e483323b742a9a75f0065466f47d0 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 30 (2024) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992024000100307&lng=en&tlng=en http://www.scielo.br/pdf/jvatitd/v30/1678-9199-jvatitd-30-e20230093.pdf https://doaj.org/toc/1678-9199 1678-9199 doi:10.1590/1678-9199-jvatitd-2023-0093 https://doaj.org/article/d49e483323b742a9a75f0065466f47d0 |
op_doi |
https://doi.org/10.1590/1678-9199-jvatitd-2023-0093 |
container_title |
Journal of Venomous Animals and Toxins including Tropical Diseases |
container_volume |
30 |
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1809897400565760000 |