Species Identification of Birds Nasal Trichobilharzia in Sari, North of Iran
Background: Cercarial dermatitis is known as an endemic parasitic disease in North of Iran, a hy- persensitive skin reaction to the penetration of nonhuman schistosome larvae into human skin. In recent studies in this region, final and intermediate hosts were recognized and Trichobilharzia was ident...
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Tehran University of Medical Sciences
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ftdoajarticles:oai:doaj.org/article:d438a40bcda243d4bc7217fef530f602 2023-05-15T13:25:00+02:00 Species Identification of Birds Nasal Trichobilharzia in Sari, North of Iran SH. Maleki A. Athari A. Haghighi N. Taghipour SH. Gohardehi S. Seyyed-Tabaei 2015-10-01T00:00:00Z https://doaj.org/article/d438a40bcda243d4bc7217fef530f602 EN eng Tehran University of Medical Sciences https://ijpa.tums.ac.ir/index.php/ijpa/article/view/287 https://doaj.org/toc/1735-7020 https://doaj.org/toc/2008-238X 1735-7020 2008-238X https://doaj.org/article/d438a40bcda243d4bc7217fef530f602 Iranian Journal of Parasitology, Vol 7, Iss 4 (2015) Trichobilharzia regenti Cercarial Dermatitis PCR Iran Anatid Infectious and parasitic diseases RC109-216 article 2015 ftdoajarticles 2022-12-31T16:27:56Z Background: Cercarial dermatitis is known as an endemic parasitic disease in North of Iran, a hy- persensitive skin reaction to the penetration of nonhuman schistosome larvae into human skin. In recent studies in this region, final and intermediate hosts were recognized and Trichobilharzia was identified as the main causative agent of cercarial dermatitis in this region, but to date the parasite species haven’t been identified. Therefore this study was performed to species identification of nasal Trichobilharzia in infected birds for the first time. Methods: A total of 45 Anas clypeata birds identified as final host, were collected from Sari in North of Iran and infected nasal tissues analyzed using molecular techniques. Genomic DNA was isolated by phenol/chloroform extraction method and ITS region of rDNA were amplified with specific pri- mers its5Trem and its4Trem, then sequenced area were compared with existing records in GenBank. Results: Twelve samples were infected with Trichobilharzia and results of PCR reaction indicated that all of them belonged to T. regenti. The sequence alignment of present work isolates and those depo- sited in GenBank showed differences in nucleotide sequences of repeat region in ITS1. Conclusion: Trichobilharzia regenti is the most frequent parasite of Anatid birds in North of Iran. This corresponds to the distribution of this parasite along the flyway of migratory birds, which annually migrate from Siberia and northern countries of Caspian Sea to wintering areas in southern regions of it. Article in Journal/Newspaper Anas clypeata Siberia Directory of Open Access Journals: DOAJ Articles |
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English |
topic |
Trichobilharzia regenti Cercarial Dermatitis PCR Iran Anatid Infectious and parasitic diseases RC109-216 |
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Trichobilharzia regenti Cercarial Dermatitis PCR Iran Anatid Infectious and parasitic diseases RC109-216 SH. Maleki A. Athari A. Haghighi N. Taghipour SH. Gohardehi S. Seyyed-Tabaei Species Identification of Birds Nasal Trichobilharzia in Sari, North of Iran |
topic_facet |
Trichobilharzia regenti Cercarial Dermatitis PCR Iran Anatid Infectious and parasitic diseases RC109-216 |
description |
Background: Cercarial dermatitis is known as an endemic parasitic disease in North of Iran, a hy- persensitive skin reaction to the penetration of nonhuman schistosome larvae into human skin. In recent studies in this region, final and intermediate hosts were recognized and Trichobilharzia was identified as the main causative agent of cercarial dermatitis in this region, but to date the parasite species haven’t been identified. Therefore this study was performed to species identification of nasal Trichobilharzia in infected birds for the first time. Methods: A total of 45 Anas clypeata birds identified as final host, were collected from Sari in North of Iran and infected nasal tissues analyzed using molecular techniques. Genomic DNA was isolated by phenol/chloroform extraction method and ITS region of rDNA were amplified with specific pri- mers its5Trem and its4Trem, then sequenced area were compared with existing records in GenBank. Results: Twelve samples were infected with Trichobilharzia and results of PCR reaction indicated that all of them belonged to T. regenti. The sequence alignment of present work isolates and those depo- sited in GenBank showed differences in nucleotide sequences of repeat region in ITS1. Conclusion: Trichobilharzia regenti is the most frequent parasite of Anatid birds in North of Iran. This corresponds to the distribution of this parasite along the flyway of migratory birds, which annually migrate from Siberia and northern countries of Caspian Sea to wintering areas in southern regions of it. |
format |
Article in Journal/Newspaper |
author |
SH. Maleki A. Athari A. Haghighi N. Taghipour SH. Gohardehi S. Seyyed-Tabaei |
author_facet |
SH. Maleki A. Athari A. Haghighi N. Taghipour SH. Gohardehi S. Seyyed-Tabaei |
author_sort |
SH. Maleki |
title |
Species Identification of Birds Nasal Trichobilharzia in Sari, North of Iran |
title_short |
Species Identification of Birds Nasal Trichobilharzia in Sari, North of Iran |
title_full |
Species Identification of Birds Nasal Trichobilharzia in Sari, North of Iran |
title_fullStr |
Species Identification of Birds Nasal Trichobilharzia in Sari, North of Iran |
title_full_unstemmed |
Species Identification of Birds Nasal Trichobilharzia in Sari, North of Iran |
title_sort |
species identification of birds nasal trichobilharzia in sari, north of iran |
publisher |
Tehran University of Medical Sciences |
publishDate |
2015 |
url |
https://doaj.org/article/d438a40bcda243d4bc7217fef530f602 |
genre |
Anas clypeata Siberia |
genre_facet |
Anas clypeata Siberia |
op_source |
Iranian Journal of Parasitology, Vol 7, Iss 4 (2015) |
op_relation |
https://ijpa.tums.ac.ir/index.php/ijpa/article/view/287 https://doaj.org/toc/1735-7020 https://doaj.org/toc/2008-238X 1735-7020 2008-238X https://doaj.org/article/d438a40bcda243d4bc7217fef530f602 |
_version_ |
1766383016140079104 |