Novel Essential Role of Ethanol Oxidation Genes at Low Temperature Revealed by Transcriptome Analysis in the Antarctic Bacterium Pseudomonas extremaustralis.
Temperature is one of the most important factors for bacterial growth and development. Cold environments are widely distributed on earth, and psychrotolerant and psychrophilic microorganisms have developed different adaptation strategies to cope with the stress derived from low temperatures. Pseudom...
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ftdoajarticles:oai:doaj.org/article:d403abfa68f844c08b1675c0af2a8daa 2023-05-15T13:46:53+02:00 Novel Essential Role of Ethanol Oxidation Genes at Low Temperature Revealed by Transcriptome Analysis in the Antarctic Bacterium Pseudomonas extremaustralis. Paula M Tribelli Esmeralda C Solar Venero Martiniano M Ricardi Maria Gómez-Lozano Laura J Raiger Iustman Søren Molin Nancy I López 2015-01-01T00:00:00Z https://doi.org/10.1371/journal.pone.0145353 https://doaj.org/article/d403abfa68f844c08b1675c0af2a8daa EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC4686015?pdf=render https://doaj.org/toc/1932-6203 1932-6203 doi:10.1371/journal.pone.0145353 https://doaj.org/article/d403abfa68f844c08b1675c0af2a8daa PLoS ONE, Vol 10, Iss 12, p e0145353 (2015) Medicine R Science Q article 2015 ftdoajarticles https://doi.org/10.1371/journal.pone.0145353 2022-12-31T11:22:09Z Temperature is one of the most important factors for bacterial growth and development. Cold environments are widely distributed on earth, and psychrotolerant and psychrophilic microorganisms have developed different adaptation strategies to cope with the stress derived from low temperatures. Pseudomonas extremaustralis is an Antarctic bacterium able to grow under low temperatures and to produce high amounts of polyhydroxyalkanoates (PHAs). In this work, we analyzed the genome-wide transcriptome by RNA deep-sequencing technology of early exponential cultures of P. extremaustralis growing in LB (Luria Broth) supplemented with sodium octanoate to favor PHA accumulation at 8°C and 30°C. We found that genes involved in primary metabolism, including tricarboxylic acid cycle (TCA) related genes, as well as cytochromes and amino acid metabolism coding genes, were repressed at low temperature. Among up-regulated genes, those coding for transcriptional regulatory and signal transduction proteins were over-represented at cold conditions. Remarkably, we found that genes involved in ethanol oxidation, exaA, exaB and exaC, encoding a pyrroloquinoline quinone (PQQ)-dependent ethanol dehydrogenase, the cytochrome c550 and an aldehyde dehydrogenase respectively, were up-regulated. Along with RNA-seq experiments, analysis of mutant strains for pqqB (PQQ biosynthesis protein B) and exaA were carried out. We found that the exaA and pqqB genes are essential for growth under low temperature in LB supplemented with sodium octanoate. Additionally, p-rosaniline assay measurements showed the presence of alcohol dehydrogenase activity at both 8°C and 30°C, while the activity was abolished in a pqqB mutant strain. These results together with the detection of ethanol by gas chromatography in P. extremaustralis cultures grown at 8°C support the conclusion that this pathway is important under cold conditions. The obtained results have led to the identification of novel components involved in cold adaptation mechanisms in this bacterium, ... Article in Journal/Newspaper Antarc* Antarctic Directory of Open Access Journals: DOAJ Articles Antarctic The Antarctic PLOS ONE 10 12 e0145353 |
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Medicine R Science Q |
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Medicine R Science Q Paula M Tribelli Esmeralda C Solar Venero Martiniano M Ricardi Maria Gómez-Lozano Laura J Raiger Iustman Søren Molin Nancy I López Novel Essential Role of Ethanol Oxidation Genes at Low Temperature Revealed by Transcriptome Analysis in the Antarctic Bacterium Pseudomonas extremaustralis. |
topic_facet |
Medicine R Science Q |
description |
Temperature is one of the most important factors for bacterial growth and development. Cold environments are widely distributed on earth, and psychrotolerant and psychrophilic microorganisms have developed different adaptation strategies to cope with the stress derived from low temperatures. Pseudomonas extremaustralis is an Antarctic bacterium able to grow under low temperatures and to produce high amounts of polyhydroxyalkanoates (PHAs). In this work, we analyzed the genome-wide transcriptome by RNA deep-sequencing technology of early exponential cultures of P. extremaustralis growing in LB (Luria Broth) supplemented with sodium octanoate to favor PHA accumulation at 8°C and 30°C. We found that genes involved in primary metabolism, including tricarboxylic acid cycle (TCA) related genes, as well as cytochromes and amino acid metabolism coding genes, were repressed at low temperature. Among up-regulated genes, those coding for transcriptional regulatory and signal transduction proteins were over-represented at cold conditions. Remarkably, we found that genes involved in ethanol oxidation, exaA, exaB and exaC, encoding a pyrroloquinoline quinone (PQQ)-dependent ethanol dehydrogenase, the cytochrome c550 and an aldehyde dehydrogenase respectively, were up-regulated. Along with RNA-seq experiments, analysis of mutant strains for pqqB (PQQ biosynthesis protein B) and exaA were carried out. We found that the exaA and pqqB genes are essential for growth under low temperature in LB supplemented with sodium octanoate. Additionally, p-rosaniline assay measurements showed the presence of alcohol dehydrogenase activity at both 8°C and 30°C, while the activity was abolished in a pqqB mutant strain. These results together with the detection of ethanol by gas chromatography in P. extremaustralis cultures grown at 8°C support the conclusion that this pathway is important under cold conditions. The obtained results have led to the identification of novel components involved in cold adaptation mechanisms in this bacterium, ... |
format |
Article in Journal/Newspaper |
author |
Paula M Tribelli Esmeralda C Solar Venero Martiniano M Ricardi Maria Gómez-Lozano Laura J Raiger Iustman Søren Molin Nancy I López |
author_facet |
Paula M Tribelli Esmeralda C Solar Venero Martiniano M Ricardi Maria Gómez-Lozano Laura J Raiger Iustman Søren Molin Nancy I López |
author_sort |
Paula M Tribelli |
title |
Novel Essential Role of Ethanol Oxidation Genes at Low Temperature Revealed by Transcriptome Analysis in the Antarctic Bacterium Pseudomonas extremaustralis. |
title_short |
Novel Essential Role of Ethanol Oxidation Genes at Low Temperature Revealed by Transcriptome Analysis in the Antarctic Bacterium Pseudomonas extremaustralis. |
title_full |
Novel Essential Role of Ethanol Oxidation Genes at Low Temperature Revealed by Transcriptome Analysis in the Antarctic Bacterium Pseudomonas extremaustralis. |
title_fullStr |
Novel Essential Role of Ethanol Oxidation Genes at Low Temperature Revealed by Transcriptome Analysis in the Antarctic Bacterium Pseudomonas extremaustralis. |
title_full_unstemmed |
Novel Essential Role of Ethanol Oxidation Genes at Low Temperature Revealed by Transcriptome Analysis in the Antarctic Bacterium Pseudomonas extremaustralis. |
title_sort |
novel essential role of ethanol oxidation genes at low temperature revealed by transcriptome analysis in the antarctic bacterium pseudomonas extremaustralis. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2015 |
url |
https://doi.org/10.1371/journal.pone.0145353 https://doaj.org/article/d403abfa68f844c08b1675c0af2a8daa |
geographic |
Antarctic The Antarctic |
geographic_facet |
Antarctic The Antarctic |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_source |
PLoS ONE, Vol 10, Iss 12, p e0145353 (2015) |
op_relation |
http://europepmc.org/articles/PMC4686015?pdf=render https://doaj.org/toc/1932-6203 1932-6203 doi:10.1371/journal.pone.0145353 https://doaj.org/article/d403abfa68f844c08b1675c0af2a8daa |
op_doi |
https://doi.org/10.1371/journal.pone.0145353 |
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PLOS ONE |
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10 |
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12 |
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e0145353 |
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1766245508918018048 |