Optimization and in Silico Analysis of a Cold-Adapted Lipase from an Antarctic Pseudomonas sp. Strain AMS8 Reaction in Triton X-100 Reverse Micelles

• Published in: Catalysts
• Main Authors: Fatin Nur Fauzi Ana Abd. Jalil, Raja Noor Zaliha Raja Abd. Rahman, Abu Bakar Salleh, Mohd Shukuri Mohamad Ali
• Format: Article in Journal/Newspaper
• Language: English
• Published: MDPI AG 2018
• Subjects: reverse micellar extraction; cold-adapted lipase; Triton X-100; molecular dynamics simulations; Chemical technology; TP1-1185; Chemistry; QD1-999; Antarctic; Triton; Antarc*
• Online Access: https://doi.org/10.3390/catal8070289; https://doaj.org/article/d3e7ed37cba2424db6bc4e6374f79fc8

A moderate yield of a purified enzyme can be achieved by using the simple technique of reverse micellar extraction (RME). RME is a liquid–liquid extraction method that uses a surfactant and an organic solvent to extract biomolecules. Instead of traditional chromatographic purification methods, which...