Evaluation of the genetic polymorphism of Plasmodium falciparum P126 protein (SERA or SERP) and its influence on naturally acquired specific antibody responses in malaria-infected individuals living in the Brazilian Amazon
Abstract Background The Plasmodium falciparum P126 protein is an asexual blood-stage malaria vaccine candidate antigen. Antibodies against P126 are able to inhibit parasite growth in vitro , and a major parasite-inhibitory epitope has been recently mapped to its 47 kDa N-terminal extremity (octamer...
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Online Access: | https://doi.org/10.1186/1475-2875-7-144 https://doaj.org/article/d3ae2f891d8447efb3c94bcc0012dc2a |
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ftdoajarticles:oai:doaj.org/article:d3ae2f891d8447efb3c94bcc0012dc2a 2023-05-15T15:18:47+02:00 Evaluation of the genetic polymorphism of Plasmodium falciparum P126 protein (SERA or SERP) and its influence on naturally acquired specific antibody responses in malaria-infected individuals living in the Brazilian Amazon Daniel-Ribeiro Cláudio T Ferreira-Da-Cruz Maria de Fátima de Simone Salvatore G Morgado Mariza G de Simone Thatiane S Santos Fátima Guimarães Monick da Silva Bruno T de Oliveira-Ferreira Joseli Sallenave-Sales Selma Pratt-Riccio Lilian Zalis Mariano G Camus Daniel Banic Dalma M 2008-07-01T00:00:00Z https://doi.org/10.1186/1475-2875-7-144 https://doaj.org/article/d3ae2f891d8447efb3c94bcc0012dc2a EN eng BMC http://www.malariajournal.com/content/7/1/144 https://doaj.org/toc/1475-2875 doi:10.1186/1475-2875-7-144 1475-2875 https://doaj.org/article/d3ae2f891d8447efb3c94bcc0012dc2a Malaria Journal, Vol 7, Iss 1, p 144 (2008) Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2008 ftdoajarticles https://doi.org/10.1186/1475-2875-7-144 2022-12-31T09:09:29Z Abstract Background The Plasmodium falciparum P126 protein is an asexual blood-stage malaria vaccine candidate antigen. Antibodies against P126 are able to inhibit parasite growth in vitro , and a major parasite-inhibitory epitope has been recently mapped to its 47 kDa N-terminal extremity (octamer repeat domain – OR domain). The OR domain basically consists of six octamer units, but variation in the sequence and number of repeat units may appear in different alleles. The aim of the present study was to investigate the polymorphism of P126 N-terminal region OR domain in P. falciparum isolates from two Brazilian malaria endemic areas and its impact on anti-OR naturally acquired antibodies. Methods The study was carried out in two villages, Candeias do Jamari (Rondonia state) and Peixoto de Azevedo (Mato Grosso state), both located in the south-western part of the Amazon region. The repetitive region of the gene encoding the P126 antigen was PCR amplified and sequenced with the di-deoxy chain termination procedure. The antibody response was evaluated by ELISA with the Nt47 synthetic peptide corresponding to the P126 OR-II domain. Results Only two types of OR fragments were identified in the studied areas, one of 175 bp (OR-I) and other of 199 bp (OR-II). A predominance of the OR-II fragment was observed in Candeias do Jamari whereas in Peixoto de Azevedo both fragments OR-I and OR-II were frequent as well as mixed infection (both fragments simultaneously) reported here for the first time. Comparing the DNA sequencing of OR-I and OR-II fragments, there was a high conservation among predicted amino acid sequences of the P126 N-terminal extremity. Data of immune response demonstrated that the OR domain is highly immunogenic in natural conditions of exposure and that the polymorphism of the OR domain does not apparently influence the specific immune response. Conclusion These findings confirm a limited genetic polymorphism of the P126 OR domain in P. falciparum isolates and that this limited genetic polymorphism does ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Serp ENVELOPE(67.100,67.100,-71.983,-71.983) Malaria Journal 7 1 144 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Daniel-Ribeiro Cláudio T Ferreira-Da-Cruz Maria de Fátima de Simone Salvatore G Morgado Mariza G de Simone Thatiane S Santos Fátima Guimarães Monick da Silva Bruno T de Oliveira-Ferreira Joseli Sallenave-Sales Selma Pratt-Riccio Lilian Zalis Mariano G Camus Daniel Banic Dalma M Evaluation of the genetic polymorphism of Plasmodium falciparum P126 protein (SERA or SERP) and its influence on naturally acquired specific antibody responses in malaria-infected individuals living in the Brazilian Amazon |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Abstract Background The Plasmodium falciparum P126 protein is an asexual blood-stage malaria vaccine candidate antigen. Antibodies against P126 are able to inhibit parasite growth in vitro , and a major parasite-inhibitory epitope has been recently mapped to its 47 kDa N-terminal extremity (octamer repeat domain – OR domain). The OR domain basically consists of six octamer units, but variation in the sequence and number of repeat units may appear in different alleles. The aim of the present study was to investigate the polymorphism of P126 N-terminal region OR domain in P. falciparum isolates from two Brazilian malaria endemic areas and its impact on anti-OR naturally acquired antibodies. Methods The study was carried out in two villages, Candeias do Jamari (Rondonia state) and Peixoto de Azevedo (Mato Grosso state), both located in the south-western part of the Amazon region. The repetitive region of the gene encoding the P126 antigen was PCR amplified and sequenced with the di-deoxy chain termination procedure. The antibody response was evaluated by ELISA with the Nt47 synthetic peptide corresponding to the P126 OR-II domain. Results Only two types of OR fragments were identified in the studied areas, one of 175 bp (OR-I) and other of 199 bp (OR-II). A predominance of the OR-II fragment was observed in Candeias do Jamari whereas in Peixoto de Azevedo both fragments OR-I and OR-II were frequent as well as mixed infection (both fragments simultaneously) reported here for the first time. Comparing the DNA sequencing of OR-I and OR-II fragments, there was a high conservation among predicted amino acid sequences of the P126 N-terminal extremity. Data of immune response demonstrated that the OR domain is highly immunogenic in natural conditions of exposure and that the polymorphism of the OR domain does not apparently influence the specific immune response. Conclusion These findings confirm a limited genetic polymorphism of the P126 OR domain in P. falciparum isolates and that this limited genetic polymorphism does ... |
format |
Article in Journal/Newspaper |
author |
Daniel-Ribeiro Cláudio T Ferreira-Da-Cruz Maria de Fátima de Simone Salvatore G Morgado Mariza G de Simone Thatiane S Santos Fátima Guimarães Monick da Silva Bruno T de Oliveira-Ferreira Joseli Sallenave-Sales Selma Pratt-Riccio Lilian Zalis Mariano G Camus Daniel Banic Dalma M |
author_facet |
Daniel-Ribeiro Cláudio T Ferreira-Da-Cruz Maria de Fátima de Simone Salvatore G Morgado Mariza G de Simone Thatiane S Santos Fátima Guimarães Monick da Silva Bruno T de Oliveira-Ferreira Joseli Sallenave-Sales Selma Pratt-Riccio Lilian Zalis Mariano G Camus Daniel Banic Dalma M |
author_sort |
Daniel-Ribeiro Cláudio T |
title |
Evaluation of the genetic polymorphism of Plasmodium falciparum P126 protein (SERA or SERP) and its influence on naturally acquired specific antibody responses in malaria-infected individuals living in the Brazilian Amazon |
title_short |
Evaluation of the genetic polymorphism of Plasmodium falciparum P126 protein (SERA or SERP) and its influence on naturally acquired specific antibody responses in malaria-infected individuals living in the Brazilian Amazon |
title_full |
Evaluation of the genetic polymorphism of Plasmodium falciparum P126 protein (SERA or SERP) and its influence on naturally acquired specific antibody responses in malaria-infected individuals living in the Brazilian Amazon |
title_fullStr |
Evaluation of the genetic polymorphism of Plasmodium falciparum P126 protein (SERA or SERP) and its influence on naturally acquired specific antibody responses in malaria-infected individuals living in the Brazilian Amazon |
title_full_unstemmed |
Evaluation of the genetic polymorphism of Plasmodium falciparum P126 protein (SERA or SERP) and its influence on naturally acquired specific antibody responses in malaria-infected individuals living in the Brazilian Amazon |
title_sort |
evaluation of the genetic polymorphism of plasmodium falciparum p126 protein (sera or serp) and its influence on naturally acquired specific antibody responses in malaria-infected individuals living in the brazilian amazon |
publisher |
BMC |
publishDate |
2008 |
url |
https://doi.org/10.1186/1475-2875-7-144 https://doaj.org/article/d3ae2f891d8447efb3c94bcc0012dc2a |
long_lat |
ENVELOPE(67.100,67.100,-71.983,-71.983) |
geographic |
Arctic Serp |
geographic_facet |
Arctic Serp |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Malaria Journal, Vol 7, Iss 1, p 144 (2008) |
op_relation |
http://www.malariajournal.com/content/7/1/144 https://doaj.org/toc/1475-2875 doi:10.1186/1475-2875-7-144 1475-2875 https://doaj.org/article/d3ae2f891d8447efb3c94bcc0012dc2a |
op_doi |
https://doi.org/10.1186/1475-2875-7-144 |
container_title |
Malaria Journal |
container_volume |
7 |
container_issue |
1 |
container_start_page |
144 |
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1766348962027012096 |