Bacterial endophyte communities in the foliage of coast redwood and giant sequoia
The endophytic bacterial microbiome, with an emerging role in plant nutrient acquisition and stress tolerance, is much less studied in natural plant populations than in agricultural crops. In a previous study, we found consistent associations between trees in the pine family and acetic acid bacteria...
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ftdoajarticles:oai:doaj.org/article:d3abd6910cdc4aa9b0ddca8315b3ad84 2023-05-15T15:02:02+02:00 Bacterial endophyte communities in the foliage of coast redwood and giant sequoia Alyssa Ann Carrell Carolin eFrank 2015-09-01T00:00:00Z https://doi.org/10.3389/fmicb.2015.01008 https://doaj.org/article/d3abd6910cdc4aa9b0ddca8315b3ad84 EN eng Frontiers Media S.A. http://journal.frontiersin.org/Journal/10.3389/fmicb.2015.01008/full https://doaj.org/toc/1664-302X 1664-302X doi:10.3389/fmicb.2015.01008 https://doaj.org/article/d3abd6910cdc4aa9b0ddca8315b3ad84 Frontiers in Microbiology, Vol 6 (2015) 16S rRNA microbiome bacterial endophytes Foliage Redwood Giant Sequoia Microbiology QR1-502 article 2015 ftdoajarticles https://doi.org/10.3389/fmicb.2015.01008 2022-12-30T21:30:02Z The endophytic bacterial microbiome, with an emerging role in plant nutrient acquisition and stress tolerance, is much less studied in natural plant populations than in agricultural crops. In a previous study, we found consistent associations between trees in the pine family and acetic acid bacteria (AAB) occurring at high relative abundance inside their needles. Our objective here was to determine if that pattern may be general to conifers, or alternatively, is more likely restricted to pines, or conifers growing in nutrient limited and exposed environments. We used 16S rRNA pyrosequencing to characterize the foliar endophyte communities of two conifers in the Cupressaceae family: Two coast redwood (Sequoia sempervirens) populations and one giant sequoia (Sequoiadendron giganteum) population were sampled. Similar to the pines, the endophyte communities of the giant trees were dominated by Proteobacteria, Firmicutes, Acidobacteria, and Actinobacteria. However, although some major OTUs occurred at a high relative abundance of 10-40% in multiple samples, no specific group of bacteria dominated the endophyte community to the extent previously observed in high-elevation pines. Several of the dominating bacterial groups in the coast redwood and giant sequoia foliage (e.g. Bacillus, Burkholderia, Actinomycetes) are known for disease- and pest suppression, raising the possibility that the endophytic microbiome protects the giant trees against biotic stress. Many of the most common and abundant OTUs in our dataset were most similar to 16S rRNA sequences from bacteria isolated from lichens or arctic plants. For example, an OTU belonging to the uncultured Rhizobiales LAR1 lineage, which is commonly associated with lichens, was observed at high relative abundance in many of the coast redwood samples. The taxa shared between the giant trees, arctic plants, and lichens may be part of a broadly defined endophyte microbiome common to temperate, boreal, and tundra ecosystems. Article in Journal/Newspaper Arctic Tundra Directory of Open Access Journals: DOAJ Articles Arctic Frontiers in Microbiology 6 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
16S rRNA microbiome bacterial endophytes Foliage Redwood Giant Sequoia Microbiology QR1-502 |
spellingShingle |
16S rRNA microbiome bacterial endophytes Foliage Redwood Giant Sequoia Microbiology QR1-502 Alyssa Ann Carrell Carolin eFrank Bacterial endophyte communities in the foliage of coast redwood and giant sequoia |
topic_facet |
16S rRNA microbiome bacterial endophytes Foliage Redwood Giant Sequoia Microbiology QR1-502 |
description |
The endophytic bacterial microbiome, with an emerging role in plant nutrient acquisition and stress tolerance, is much less studied in natural plant populations than in agricultural crops. In a previous study, we found consistent associations between trees in the pine family and acetic acid bacteria (AAB) occurring at high relative abundance inside their needles. Our objective here was to determine if that pattern may be general to conifers, or alternatively, is more likely restricted to pines, or conifers growing in nutrient limited and exposed environments. We used 16S rRNA pyrosequencing to characterize the foliar endophyte communities of two conifers in the Cupressaceae family: Two coast redwood (Sequoia sempervirens) populations and one giant sequoia (Sequoiadendron giganteum) population were sampled. Similar to the pines, the endophyte communities of the giant trees were dominated by Proteobacteria, Firmicutes, Acidobacteria, and Actinobacteria. However, although some major OTUs occurred at a high relative abundance of 10-40% in multiple samples, no specific group of bacteria dominated the endophyte community to the extent previously observed in high-elevation pines. Several of the dominating bacterial groups in the coast redwood and giant sequoia foliage (e.g. Bacillus, Burkholderia, Actinomycetes) are known for disease- and pest suppression, raising the possibility that the endophytic microbiome protects the giant trees against biotic stress. Many of the most common and abundant OTUs in our dataset were most similar to 16S rRNA sequences from bacteria isolated from lichens or arctic plants. For example, an OTU belonging to the uncultured Rhizobiales LAR1 lineage, which is commonly associated with lichens, was observed at high relative abundance in many of the coast redwood samples. The taxa shared between the giant trees, arctic plants, and lichens may be part of a broadly defined endophyte microbiome common to temperate, boreal, and tundra ecosystems. |
format |
Article in Journal/Newspaper |
author |
Alyssa Ann Carrell Carolin eFrank |
author_facet |
Alyssa Ann Carrell Carolin eFrank |
author_sort |
Alyssa Ann Carrell |
title |
Bacterial endophyte communities in the foliage of coast redwood and giant sequoia |
title_short |
Bacterial endophyte communities in the foliage of coast redwood and giant sequoia |
title_full |
Bacterial endophyte communities in the foliage of coast redwood and giant sequoia |
title_fullStr |
Bacterial endophyte communities in the foliage of coast redwood and giant sequoia |
title_full_unstemmed |
Bacterial endophyte communities in the foliage of coast redwood and giant sequoia |
title_sort |
bacterial endophyte communities in the foliage of coast redwood and giant sequoia |
publisher |
Frontiers Media S.A. |
publishDate |
2015 |
url |
https://doi.org/10.3389/fmicb.2015.01008 https://doaj.org/article/d3abd6910cdc4aa9b0ddca8315b3ad84 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic Tundra |
genre_facet |
Arctic Tundra |
op_source |
Frontiers in Microbiology, Vol 6 (2015) |
op_relation |
http://journal.frontiersin.org/Journal/10.3389/fmicb.2015.01008/full https://doaj.org/toc/1664-302X 1664-302X doi:10.3389/fmicb.2015.01008 https://doaj.org/article/d3abd6910cdc4aa9b0ddca8315b3ad84 |
op_doi |
https://doi.org/10.3389/fmicb.2015.01008 |
container_title |
Frontiers in Microbiology |
container_volume |
6 |
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1766334026501586944 |