Bacterial endophyte communities in the foliage of coast redwood and giant sequoia

• Published in: Frontiers in Microbiology
• Main Authors: Alyssa Ann Carrell, Carolin eFrank
• Format: Article in Journal/Newspaper
• Language: English
• Published: Frontiers Media S.A. 2015
• Subjects: 16S rRNA; microbiome; bacterial endophytes; Foliage; Redwood; Giant Sequoia; Microbiology; QR1-502; Arctic; Tundra
• Online Access: https://doi.org/10.3389/fmicb.2015.01008; https://doaj.org/article/d3abd6910cdc4aa9b0ddca8315b3ad84

The endophytic bacterial microbiome, with an emerging role in plant nutrient acquisition and stress tolerance, is much less studied in natural plant populations than in agricultural crops. In a previous study, we found consistent associations between trees in the pine family and acetic acid bacteria (AAB) occurring at high relative abundance inside their needles. Our objective here was to determine if that pattern may be general to conifers, or alternatively, is more likely restricted to pines, or conifers growing in nutrient limited and exposed environments. We used 16S rRNA pyrosequencing to characterize the foliar endophyte communities of two conifers in the Cupressaceae family: Two coast redwood (Sequoia sempervirens) populations and one giant sequoia (Sequoiadendron giganteum) population were sampled. Similar to the pines, the endophyte communities of the giant trees were dominated by Proteobacteria, Firmicutes, Acidobacteria, and Actinobacteria. However, although some major OTUs occurred at a high relative abundance of 10-40% in multiple samples, no specific group of bacteria dominated the endophyte community to the extent previously observed in high-elevation pines. Several of the dominating bacterial groups in the coast redwood and giant sequoia foliage (e.g. Bacillus, Burkholderia, Actinomycetes) are known for disease- and pest suppression, raising the possibility that the endophytic microbiome protects the giant trees against biotic stress. Many of the most common and abundant OTUs in our dataset were most similar to 16S rRNA sequences from bacteria isolated from lichens or arctic plants. For example, an OTU belonging to the uncultured Rhizobiales LAR1 lineage, which is commonly associated with lichens, was observed at high relative abundance in many of the coast redwood samples. The taxa shared between the giant trees, arctic plants, and lichens may be part of a broadly defined endophyte microbiome common to temperate, boreal, and tundra ecosystems.