Genome-Wide Identification of Aqp Family Related to Spermatogenesis in Turbot ( Scophthalmus maximus )
The development and maturation of sperm entails intricate metabolic processes involving water molecules, amino acids, hormones, and various substances. Among these processes, the role of aquaporins ( aqps ) in the testis is crucial. Turbot ( Scophthalmus maximus ) is a significant marine flatfish sp...
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ftdoajarticles:oai:doaj.org/article:d3ab699d008f41a88e60c3acba90b548 2023-08-20T04:09:40+02:00 Genome-Wide Identification of Aqp Family Related to Spermatogenesis in Turbot ( Scophthalmus maximus ) Xueying Wang Ning Zhao Tao Wang Jinwei Huang Qinghua Liu Jun Li 2023-07-01T00:00:00Z https://doi.org/10.3390/ijms241411770 https://doaj.org/article/d3ab699d008f41a88e60c3acba90b548 EN eng MDPI AG https://www.mdpi.com/1422-0067/24/14/11770 https://doaj.org/toc/1661-6596 https://doaj.org/toc/1422-0067 doi:10.3390/ijms241411770 1422-0067 1661-6596 https://doaj.org/article/d3ab699d008f41a88e60c3acba90b548 International Journal of Molecular Sciences, Vol 24, Iss 11770, p 11770 (2023) turbot aqps spermatogenesis sperm Biology (General) QH301-705.5 Chemistry QD1-999 article 2023 ftdoajarticles https://doi.org/10.3390/ijms241411770 2023-07-30T00:35:34Z The development and maturation of sperm entails intricate metabolic processes involving water molecules, amino acids, hormones, and various substances. Among these processes, the role of aquaporins ( aqps ) in the testis is crucial. Turbot ( Scophthalmus maximus ) is a significant marine flatfish species in China; however, natural egg laying in females is not feasible under cultured conditions. Consequently, artificial insemination becomes necessary, requiring the retrieval of sperm and eggs through artificial methods. In this study, we combined genomic, transcriptomics, RT-qPCR, computer-assisted sperm analysis (CASA), and immunohistochemistry to investigate the involvement of the aqp family in spermatogenesis in turbot. Through genomic data analysis, we identified 16 aqps genes dispersed across 13 chromosomes, each exhibiting the characteristic major intrinsic protein (MIP) domain associated with AQPs. The results from RNA-seq and RT-qPCR analysis revealed prominent expression of aqp4 , 10 , and 12 during the proliferative stage, whereas aqp1 showed primary expression during the mature stage. aqp11 displayed high expression levels during both MSII and MSV stages, potentially contributing significantly to the proliferation and maturation of male germ cells. Conversely, aqp8 showed elevated expression levels during the MSIII, MSIII-IV, and MSIV stages, suggesting its direct involvement in spermiogenesis. Immunohistochemical analysis unveiled the predominant localization of AQP1 protein in male germ cells rather than Sertoli cells, specifically concentrated in the head of sperm within cysts. Furthermore, a noteworthy decline in sperm motility was observed when sperm were subjected to treatment with either the AQP1-specific inhibitor (HgCl 2 ) or the AQP1 antibody. However, no direct correlation was found between the expression of Smaqp1 and sperm quality. Overall, these findings provide new insights into the involvement of aqps in teleost spermatogenesis. Moreover, they hold potential for improving techniques ... Article in Journal/Newspaper Scophthalmus maximus Turbot Directory of Open Access Journals: DOAJ Articles International Journal of Molecular Sciences 24 14 11770 |
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turbot aqps spermatogenesis sperm Biology (General) QH301-705.5 Chemistry QD1-999 |
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turbot aqps spermatogenesis sperm Biology (General) QH301-705.5 Chemistry QD1-999 Xueying Wang Ning Zhao Tao Wang Jinwei Huang Qinghua Liu Jun Li Genome-Wide Identification of Aqp Family Related to Spermatogenesis in Turbot ( Scophthalmus maximus ) |
topic_facet |
turbot aqps spermatogenesis sperm Biology (General) QH301-705.5 Chemistry QD1-999 |
description |
The development and maturation of sperm entails intricate metabolic processes involving water molecules, amino acids, hormones, and various substances. Among these processes, the role of aquaporins ( aqps ) in the testis is crucial. Turbot ( Scophthalmus maximus ) is a significant marine flatfish species in China; however, natural egg laying in females is not feasible under cultured conditions. Consequently, artificial insemination becomes necessary, requiring the retrieval of sperm and eggs through artificial methods. In this study, we combined genomic, transcriptomics, RT-qPCR, computer-assisted sperm analysis (CASA), and immunohistochemistry to investigate the involvement of the aqp family in spermatogenesis in turbot. Through genomic data analysis, we identified 16 aqps genes dispersed across 13 chromosomes, each exhibiting the characteristic major intrinsic protein (MIP) domain associated with AQPs. The results from RNA-seq and RT-qPCR analysis revealed prominent expression of aqp4 , 10 , and 12 during the proliferative stage, whereas aqp1 showed primary expression during the mature stage. aqp11 displayed high expression levels during both MSII and MSV stages, potentially contributing significantly to the proliferation and maturation of male germ cells. Conversely, aqp8 showed elevated expression levels during the MSIII, MSIII-IV, and MSIV stages, suggesting its direct involvement in spermiogenesis. Immunohistochemical analysis unveiled the predominant localization of AQP1 protein in male germ cells rather than Sertoli cells, specifically concentrated in the head of sperm within cysts. Furthermore, a noteworthy decline in sperm motility was observed when sperm were subjected to treatment with either the AQP1-specific inhibitor (HgCl 2 ) or the AQP1 antibody. However, no direct correlation was found between the expression of Smaqp1 and sperm quality. Overall, these findings provide new insights into the involvement of aqps in teleost spermatogenesis. Moreover, they hold potential for improving techniques ... |
format |
Article in Journal/Newspaper |
author |
Xueying Wang Ning Zhao Tao Wang Jinwei Huang Qinghua Liu Jun Li |
author_facet |
Xueying Wang Ning Zhao Tao Wang Jinwei Huang Qinghua Liu Jun Li |
author_sort |
Xueying Wang |
title |
Genome-Wide Identification of Aqp Family Related to Spermatogenesis in Turbot ( Scophthalmus maximus ) |
title_short |
Genome-Wide Identification of Aqp Family Related to Spermatogenesis in Turbot ( Scophthalmus maximus ) |
title_full |
Genome-Wide Identification of Aqp Family Related to Spermatogenesis in Turbot ( Scophthalmus maximus ) |
title_fullStr |
Genome-Wide Identification of Aqp Family Related to Spermatogenesis in Turbot ( Scophthalmus maximus ) |
title_full_unstemmed |
Genome-Wide Identification of Aqp Family Related to Spermatogenesis in Turbot ( Scophthalmus maximus ) |
title_sort |
genome-wide identification of aqp family related to spermatogenesis in turbot ( scophthalmus maximus ) |
publisher |
MDPI AG |
publishDate |
2023 |
url |
https://doi.org/10.3390/ijms241411770 https://doaj.org/article/d3ab699d008f41a88e60c3acba90b548 |
genre |
Scophthalmus maximus Turbot |
genre_facet |
Scophthalmus maximus Turbot |
op_source |
International Journal of Molecular Sciences, Vol 24, Iss 11770, p 11770 (2023) |
op_relation |
https://www.mdpi.com/1422-0067/24/14/11770 https://doaj.org/toc/1661-6596 https://doaj.org/toc/1422-0067 doi:10.3390/ijms241411770 1422-0067 1661-6596 https://doaj.org/article/d3ab699d008f41a88e60c3acba90b548 |
op_doi |
https://doi.org/10.3390/ijms241411770 |
container_title |
International Journal of Molecular Sciences |
container_volume |
24 |
container_issue |
14 |
container_start_page |
11770 |
_version_ |
1774723270561497088 |