Comparison of diagnostic methods for the detection and quantification of the four sympatric Plasmodium species in field samples from Papua New Guinea
Abstract Background Accurate diagnosis of Plasmodium infections is essential for malaria morbidity and mortality reduction in tropical areas. Despite great advantages of light microscopy (LM) for malaria diagnosis, its limited sensitivity is a critical shortfall for epidemiological studies. Robust m...
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ftdoajarticles:oai:doaj.org/article:d39c61b312ed4bbfa35827514115c73b 2023-05-15T15:18:33+02:00 Comparison of diagnostic methods for the detection and quantification of the four sympatric Plasmodium species in field samples from Papua New Guinea Zimmerman Peter A Iga Jonah Barnadas Céline Betuela Inoni Mueller Dania Rosanas-Urgell Anna del Portillo Hernando A Siba Peter Mueller Ivo Felger Ingrid 2010-12-01T00:00:00Z https://doi.org/10.1186/1475-2875-9-361 https://doaj.org/article/d39c61b312ed4bbfa35827514115c73b EN eng BMC http://www.malariajournal.com/content/9/1/361 https://doaj.org/toc/1475-2875 doi:10.1186/1475-2875-9-361 1475-2875 https://doaj.org/article/d39c61b312ed4bbfa35827514115c73b Malaria Journal, Vol 9, Iss 1, p 361 (2010) Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2010 ftdoajarticles https://doi.org/10.1186/1475-2875-9-361 2022-12-31T08:35:55Z Abstract Background Accurate diagnosis of Plasmodium infections is essential for malaria morbidity and mortality reduction in tropical areas. Despite great advantages of light microscopy (LM) for malaria diagnosis, its limited sensitivity is a critical shortfall for epidemiological studies. Robust molecular diagnostics tools are thus needed. Methods The present study describes the development of a duplex quantitative real time PCR (qPCR) assay, which specifically detects and quantifies the four human Plasmodium species. Performance of this method was compared to PCR-ligase detection reaction-fluorescent microsphere assay (PCR_LDR_FMA), nested PCR (nPCR) and LM, using field samples collected from 452 children one to five years of age from the Sepik area in Papua New Guinea. Agreement between diagnostic methods was calcualted using kappa statistics. Results The agreement of qPCR with other molecular diagnostic methods was substantial for the detection of P. falciparum , but was moderate for the detection of P. vivax , P. malariae and P. ovale . P. falciparum and P. vivax prevalence by qPCR was 40.9% and 65.7% respectively. This compares to 43.8% and 73.2% by nPCR and 47.1% and 67.5% by PCR_LDR_FMA. P. malariae and P. ovale prevalence was 4.7% and 7.3% by qPCR, 3.3% and 3.8% by nPCR, and 7.7% and 4.4% by PCR_LDR_FMA. Prevalence by LM was lower for all four species, being 25.4% for P. falciparum , 54.9% for P. vivax , 2.4% for P. malariae and 0.0% for P. ovale . The quantification by qPCR closely correlated with microscopic quantification for P. falciparum and P. vivax samples (R2 = 0.825 and R2 = 0.505, respectively). The low prevalence of P. malariae and P. ovale did not permit a solid comparative analysis of quantification for these species. Conclusions The qPCR assay developed proved optimal for detection of all four Plasmodium species. Densities by LM were well reflected in quantification results by qPCR, whereby congruence was better for P. falciparum than for P. vivax . This likely is a consequence of the ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Malaria Journal 9 1 |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
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English |
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Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
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Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Zimmerman Peter A Iga Jonah Barnadas Céline Betuela Inoni Mueller Dania Rosanas-Urgell Anna del Portillo Hernando A Siba Peter Mueller Ivo Felger Ingrid Comparison of diagnostic methods for the detection and quantification of the four sympatric Plasmodium species in field samples from Papua New Guinea |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Abstract Background Accurate diagnosis of Plasmodium infections is essential for malaria morbidity and mortality reduction in tropical areas. Despite great advantages of light microscopy (LM) for malaria diagnosis, its limited sensitivity is a critical shortfall for epidemiological studies. Robust molecular diagnostics tools are thus needed. Methods The present study describes the development of a duplex quantitative real time PCR (qPCR) assay, which specifically detects and quantifies the four human Plasmodium species. Performance of this method was compared to PCR-ligase detection reaction-fluorescent microsphere assay (PCR_LDR_FMA), nested PCR (nPCR) and LM, using field samples collected from 452 children one to five years of age from the Sepik area in Papua New Guinea. Agreement between diagnostic methods was calcualted using kappa statistics. Results The agreement of qPCR with other molecular diagnostic methods was substantial for the detection of P. falciparum , but was moderate for the detection of P. vivax , P. malariae and P. ovale . P. falciparum and P. vivax prevalence by qPCR was 40.9% and 65.7% respectively. This compares to 43.8% and 73.2% by nPCR and 47.1% and 67.5% by PCR_LDR_FMA. P. malariae and P. ovale prevalence was 4.7% and 7.3% by qPCR, 3.3% and 3.8% by nPCR, and 7.7% and 4.4% by PCR_LDR_FMA. Prevalence by LM was lower for all four species, being 25.4% for P. falciparum , 54.9% for P. vivax , 2.4% for P. malariae and 0.0% for P. ovale . The quantification by qPCR closely correlated with microscopic quantification for P. falciparum and P. vivax samples (R2 = 0.825 and R2 = 0.505, respectively). The low prevalence of P. malariae and P. ovale did not permit a solid comparative analysis of quantification for these species. Conclusions The qPCR assay developed proved optimal for detection of all four Plasmodium species. Densities by LM were well reflected in quantification results by qPCR, whereby congruence was better for P. falciparum than for P. vivax . This likely is a consequence of the ... |
format |
Article in Journal/Newspaper |
author |
Zimmerman Peter A Iga Jonah Barnadas Céline Betuela Inoni Mueller Dania Rosanas-Urgell Anna del Portillo Hernando A Siba Peter Mueller Ivo Felger Ingrid |
author_facet |
Zimmerman Peter A Iga Jonah Barnadas Céline Betuela Inoni Mueller Dania Rosanas-Urgell Anna del Portillo Hernando A Siba Peter Mueller Ivo Felger Ingrid |
author_sort |
Zimmerman Peter A |
title |
Comparison of diagnostic methods for the detection and quantification of the four sympatric Plasmodium species in field samples from Papua New Guinea |
title_short |
Comparison of diagnostic methods for the detection and quantification of the four sympatric Plasmodium species in field samples from Papua New Guinea |
title_full |
Comparison of diagnostic methods for the detection and quantification of the four sympatric Plasmodium species in field samples from Papua New Guinea |
title_fullStr |
Comparison of diagnostic methods for the detection and quantification of the four sympatric Plasmodium species in field samples from Papua New Guinea |
title_full_unstemmed |
Comparison of diagnostic methods for the detection and quantification of the four sympatric Plasmodium species in field samples from Papua New Guinea |
title_sort |
comparison of diagnostic methods for the detection and quantification of the four sympatric plasmodium species in field samples from papua new guinea |
publisher |
BMC |
publishDate |
2010 |
url |
https://doi.org/10.1186/1475-2875-9-361 https://doaj.org/article/d39c61b312ed4bbfa35827514115c73b |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Malaria Journal, Vol 9, Iss 1, p 361 (2010) |
op_relation |
http://www.malariajournal.com/content/9/1/361 https://doaj.org/toc/1475-2875 doi:10.1186/1475-2875-9-361 1475-2875 https://doaj.org/article/d39c61b312ed4bbfa35827514115c73b |
op_doi |
https://doi.org/10.1186/1475-2875-9-361 |
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Malaria Journal |
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9 |
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1 |
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1766348743726071808 |