Evaluation of serological diagnostic test systems assessing the immune response to Japanese encephalitis vaccination.
A new commercial anti-Japanese encephalitis virus IgM and IgG indirect immunofluorescence test (IIFT) was evaluated for the detection of the humoral immune response after Japanese encephalitis vaccination. The IgM IIFT was compared to two IgM capture ELISAs and the IgG IIFT was analysed in compariso...
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ftdoajarticles:oai:doaj.org/article:d381d634f4764f228ecedf126c87830f 2023-05-15T15:15:05+02:00 Evaluation of serological diagnostic test systems assessing the immune response to Japanese encephalitis vaccination. Nadine Litzba Christoph S Klade Sabine Lederer Matthias Niedrig 2010-01-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0000883 https://doaj.org/article/d381d634f4764f228ecedf126c87830f EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC2982812?pdf=render https://doaj.org/toc/1935-2735 1935-2735 doi:10.1371/journal.pntd.0000883 https://doaj.org/article/d381d634f4764f228ecedf126c87830f PLoS Neglected Tropical Diseases, Vol 4, Iss 11, p e883 (2010) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2010 ftdoajarticles https://doi.org/10.1371/journal.pntd.0000883 2022-12-31T03:38:12Z A new commercial anti-Japanese encephalitis virus IgM and IgG indirect immunofluorescence test (IIFT) was evaluated for the detection of the humoral immune response after Japanese encephalitis vaccination. The IgM IIFT was compared to two IgM capture ELISAs and the IgG IIFT was analysed in comparison to a plaque reduction neutralization test (PRNT50) and an IgG ELISA. Moreover, the course of the immune reaction after vaccination with an inactivated JEV vaccine was examined. For the present study 300 serum samples from different blood withdrawals from 100 persons vaccinated against Japanese encephalitis were used. For the IgM evaluation, altogether 78 PRNT50 positive samples taken 7 to 56 days after vaccination and 78 PRNT50 negative sera were analyzed with the Euroimmun anti-JEV IgM IIFT, the Panbio Japanese Encephalitis - Dengue IgM Combo ELISA and the InBios JE Detect IgM capture ELISA. For the IgG evaluation, 100 sera taken 56 days after vaccination and 100 corresponding sera taken before vaccination were tested in the PRNT50, the Euroimmun anti-JEV IgG IIFT, and the InBios JE Detect IgG ELISA. The Euroimmun IgM IIFT showed in comparison to the Panbio ELISA a specificity of 95% and a sensitivity of 86%. With respect to the InBios ELISA, the values were 100% and 83.9%, respectively. The analysis of the Euroimmun IgG IIFT performance and the PRNT50 results demonstrated a specificity of 100% and a sensitivity of 93.8%, whereas it was not possible to detect more than 6.6% of the PRNT50 positive sera as positive with the InBios JE Detect IgG ELISA. Thus, the IIFT is a valuable alternative to the established methods in detecting anti-JEV antibodies after vaccination in travellers and it might prove useful for the diagnosis of acutely infected persons. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLoS Neglected Tropical Diseases 4 11 e883 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Nadine Litzba Christoph S Klade Sabine Lederer Matthias Niedrig Evaluation of serological diagnostic test systems assessing the immune response to Japanese encephalitis vaccination. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
A new commercial anti-Japanese encephalitis virus IgM and IgG indirect immunofluorescence test (IIFT) was evaluated for the detection of the humoral immune response after Japanese encephalitis vaccination. The IgM IIFT was compared to two IgM capture ELISAs and the IgG IIFT was analysed in comparison to a plaque reduction neutralization test (PRNT50) and an IgG ELISA. Moreover, the course of the immune reaction after vaccination with an inactivated JEV vaccine was examined. For the present study 300 serum samples from different blood withdrawals from 100 persons vaccinated against Japanese encephalitis were used. For the IgM evaluation, altogether 78 PRNT50 positive samples taken 7 to 56 days after vaccination and 78 PRNT50 negative sera were analyzed with the Euroimmun anti-JEV IgM IIFT, the Panbio Japanese Encephalitis - Dengue IgM Combo ELISA and the InBios JE Detect IgM capture ELISA. For the IgG evaluation, 100 sera taken 56 days after vaccination and 100 corresponding sera taken before vaccination were tested in the PRNT50, the Euroimmun anti-JEV IgG IIFT, and the InBios JE Detect IgG ELISA. The Euroimmun IgM IIFT showed in comparison to the Panbio ELISA a specificity of 95% and a sensitivity of 86%. With respect to the InBios ELISA, the values were 100% and 83.9%, respectively. The analysis of the Euroimmun IgG IIFT performance and the PRNT50 results demonstrated a specificity of 100% and a sensitivity of 93.8%, whereas it was not possible to detect more than 6.6% of the PRNT50 positive sera as positive with the InBios JE Detect IgG ELISA. Thus, the IIFT is a valuable alternative to the established methods in detecting anti-JEV antibodies after vaccination in travellers and it might prove useful for the diagnosis of acutely infected persons. |
format |
Article in Journal/Newspaper |
author |
Nadine Litzba Christoph S Klade Sabine Lederer Matthias Niedrig |
author_facet |
Nadine Litzba Christoph S Klade Sabine Lederer Matthias Niedrig |
author_sort |
Nadine Litzba |
title |
Evaluation of serological diagnostic test systems assessing the immune response to Japanese encephalitis vaccination. |
title_short |
Evaluation of serological diagnostic test systems assessing the immune response to Japanese encephalitis vaccination. |
title_full |
Evaluation of serological diagnostic test systems assessing the immune response to Japanese encephalitis vaccination. |
title_fullStr |
Evaluation of serological diagnostic test systems assessing the immune response to Japanese encephalitis vaccination. |
title_full_unstemmed |
Evaluation of serological diagnostic test systems assessing the immune response to Japanese encephalitis vaccination. |
title_sort |
evaluation of serological diagnostic test systems assessing the immune response to japanese encephalitis vaccination. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2010 |
url |
https://doi.org/10.1371/journal.pntd.0000883 https://doaj.org/article/d381d634f4764f228ecedf126c87830f |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 4, Iss 11, p e883 (2010) |
op_relation |
http://europepmc.org/articles/PMC2982812?pdf=render https://doaj.org/toc/1935-2735 1935-2735 doi:10.1371/journal.pntd.0000883 https://doaj.org/article/d381d634f4764f228ecedf126c87830f |
op_doi |
https://doi.org/10.1371/journal.pntd.0000883 |
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PLoS Neglected Tropical Diseases |
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container_issue |
11 |
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e883 |
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