Comparative proteomics reveals Cryptosporidium parvum manipulation of the host cell molecular expression and immune response.
Cryptosporidium is a life-threating protozoan parasite belonging to the phylum Apicomplexa, which mainly causes gastroenteritis in a variety of vertebrate hosts. Currently, there is a re-emergence of Cryptosporidium infection; however, no fully effective drug or vaccine is available to treat Cryptos...
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ftdoajarticles:oai:doaj.org/article:d375035e32f348329b3ef35bda0c9eb8 2023-05-15T15:08:04+02:00 Comparative proteomics reveals Cryptosporidium parvum manipulation of the host cell molecular expression and immune response. Teng Li Hua Liu Nan Jiang Yiluo Wang Ying Wang Jing Zhang Yujuan Shen Jianping Cao 2021-11-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0009949 https://doaj.org/article/d375035e32f348329b3ef35bda0c9eb8 EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pntd.0009949 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0009949 https://doaj.org/article/d375035e32f348329b3ef35bda0c9eb8 PLoS Neglected Tropical Diseases, Vol 15, Iss 11, p e0009949 (2021) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2021 ftdoajarticles https://doi.org/10.1371/journal.pntd.0009949 2022-12-30T20:37:25Z Cryptosporidium is a life-threating protozoan parasite belonging to the phylum Apicomplexa, which mainly causes gastroenteritis in a variety of vertebrate hosts. Currently, there is a re-emergence of Cryptosporidium infection; however, no fully effective drug or vaccine is available to treat Cryptosporidiosis. In the present study, to better understand the detailed interaction between the host and Cryptosporidium parvum, a large-scale label-free proteomics study was conducted to characterize the changes to the proteome induced by C. parvum infection. Among 4406 proteins identified, 121 proteins were identified as differentially abundant (> 1.5-fold cutoff, P < 0.05) in C. parvum infected HCT-8 cells compared with uninfected cells. Among them, 67 proteins were upregulated, and 54 proteins were downregulated at 36 h post infection. Analysis of the differentially abundant proteins revealed an interferon-centered immune response of the host cells against C. parvum infection and extensive inhibition of metabolism-related enzymes in the host cells caused by infection. Several proteins were further verified using quantitative real-time reverse transcription polymerase chain reaction and western blotting. This systematic analysis of the proteomics of C. parvum-infected HCT-8 cells identified a wide range of functional proteins that participate in host anti-parasite immunity or act as potential targets during infection, providing new insights into the molecular mechanism of C. parvum infection. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 15 11 e0009949 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
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English |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Teng Li Hua Liu Nan Jiang Yiluo Wang Ying Wang Jing Zhang Yujuan Shen Jianping Cao Comparative proteomics reveals Cryptosporidium parvum manipulation of the host cell molecular expression and immune response. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Cryptosporidium is a life-threating protozoan parasite belonging to the phylum Apicomplexa, which mainly causes gastroenteritis in a variety of vertebrate hosts. Currently, there is a re-emergence of Cryptosporidium infection; however, no fully effective drug or vaccine is available to treat Cryptosporidiosis. In the present study, to better understand the detailed interaction between the host and Cryptosporidium parvum, a large-scale label-free proteomics study was conducted to characterize the changes to the proteome induced by C. parvum infection. Among 4406 proteins identified, 121 proteins were identified as differentially abundant (> 1.5-fold cutoff, P < 0.05) in C. parvum infected HCT-8 cells compared with uninfected cells. Among them, 67 proteins were upregulated, and 54 proteins were downregulated at 36 h post infection. Analysis of the differentially abundant proteins revealed an interferon-centered immune response of the host cells against C. parvum infection and extensive inhibition of metabolism-related enzymes in the host cells caused by infection. Several proteins were further verified using quantitative real-time reverse transcription polymerase chain reaction and western blotting. This systematic analysis of the proteomics of C. parvum-infected HCT-8 cells identified a wide range of functional proteins that participate in host anti-parasite immunity or act as potential targets during infection, providing new insights into the molecular mechanism of C. parvum infection. |
format |
Article in Journal/Newspaper |
author |
Teng Li Hua Liu Nan Jiang Yiluo Wang Ying Wang Jing Zhang Yujuan Shen Jianping Cao |
author_facet |
Teng Li Hua Liu Nan Jiang Yiluo Wang Ying Wang Jing Zhang Yujuan Shen Jianping Cao |
author_sort |
Teng Li |
title |
Comparative proteomics reveals Cryptosporidium parvum manipulation of the host cell molecular expression and immune response. |
title_short |
Comparative proteomics reveals Cryptosporidium parvum manipulation of the host cell molecular expression and immune response. |
title_full |
Comparative proteomics reveals Cryptosporidium parvum manipulation of the host cell molecular expression and immune response. |
title_fullStr |
Comparative proteomics reveals Cryptosporidium parvum manipulation of the host cell molecular expression and immune response. |
title_full_unstemmed |
Comparative proteomics reveals Cryptosporidium parvum manipulation of the host cell molecular expression and immune response. |
title_sort |
comparative proteomics reveals cryptosporidium parvum manipulation of the host cell molecular expression and immune response. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2021 |
url |
https://doi.org/10.1371/journal.pntd.0009949 https://doaj.org/article/d375035e32f348329b3ef35bda0c9eb8 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 15, Iss 11, p e0009949 (2021) |
op_relation |
https://doi.org/10.1371/journal.pntd.0009949 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0009949 https://doaj.org/article/d375035e32f348329b3ef35bda0c9eb8 |
op_doi |
https://doi.org/10.1371/journal.pntd.0009949 |
container_title |
PLOS Neglected Tropical Diseases |
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15 |
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11 |
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e0009949 |
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1766339500326256640 |