Production, purification and characterization of bacteriocin from Lactobacillus murinus AU06 and its broad antibacterial spectrum
Objective: To study the production, purification and characterization of bacteriocin from Lactobacillus murinus AU06 isolated from marine sediments and its broad spectrum of inhibition against fish pathogens. Methods: The selected strain was used in production, purification and characterized of bact...
Published in: | Asian Pacific Journal of Tropical Biomedicine |
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Wolters Kluwer Medknow Publications
2014
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ftdoajarticles:oai:doaj.org/article:d36cd2ddcfd1465884cc7c6e4a595cfd 2023-05-15T15:05:12+02:00 Production, purification and characterization of bacteriocin from Lactobacillus murinus AU06 and its broad antibacterial spectrum Sivaramasamy Elayaraja Neelamegam Annamalai Packiyam Mayavu Thangavel Balasubramanian 2014-05-01T00:00:00Z https://doi.org/10.12980/APJTB.4.2014C537 https://doaj.org/article/d36cd2ddcfd1465884cc7c6e4a595cfd EN eng Wolters Kluwer Medknow Publications http://www.sciencedirect.com/science/article/pii/S2221169115302872 https://doaj.org/toc/2221-1691 2221-1691 doi:10.12980/APJTB.4.2014C537 https://doaj.org/article/d36cd2ddcfd1465884cc7c6e4a595cfd Asian Pacific Journal of Tropical Biomedicine, Vol 4, Iss S1, Pp S305-S311 (2014) Lactobacillus murinus Bacteriocin Purification Antibacterial Pathogen Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 article 2014 ftdoajarticles https://doi.org/10.12980/APJTB.4.2014C537 2022-12-31T09:16:08Z Objective: To study the production, purification and characterization of bacteriocin from Lactobacillus murinus AU06 isolated from marine sediments and its broad spectrum of inhibition against fish pathogens. Methods: The selected strain was used in production, purification and characterized of bacteriocin. In addition, purified bacteriocin was tested for its antimicrobial activity against fish pathogens. Results: In the present study, the bacteriocin production was found to be higher at 35 °C, pH 6.0 and was purified to 4.74 fold with 55. 38 U/mg of specific activity with the yield of 28.92%. The molecular weight of the purified bacteriocin was estimated as 21 kDa. The purified bacteriocin exhibited complete inactivation of antimicrobial activity when treated with proteinase K, pronase, chymotrypsin, trypsin, pepsin and papain. The purified bacteriocin exhibited broad inhibitory spectrum against both Gram positive and negative bacteria. Conclusions: It is concluded that the ability of bacteriocin in inhibiting a wide-range of pathogenic bacteria is of potential interest for food safety and may have future applications in food preservative. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Asian Pacific Journal of Tropical Biomedicine 4 S305 S311 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Lactobacillus murinus Bacteriocin Purification Antibacterial Pathogen Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
spellingShingle |
Lactobacillus murinus Bacteriocin Purification Antibacterial Pathogen Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 Sivaramasamy Elayaraja Neelamegam Annamalai Packiyam Mayavu Thangavel Balasubramanian Production, purification and characterization of bacteriocin from Lactobacillus murinus AU06 and its broad antibacterial spectrum |
topic_facet |
Lactobacillus murinus Bacteriocin Purification Antibacterial Pathogen Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
description |
Objective: To study the production, purification and characterization of bacteriocin from Lactobacillus murinus AU06 isolated from marine sediments and its broad spectrum of inhibition against fish pathogens. Methods: The selected strain was used in production, purification and characterized of bacteriocin. In addition, purified bacteriocin was tested for its antimicrobial activity against fish pathogens. Results: In the present study, the bacteriocin production was found to be higher at 35 °C, pH 6.0 and was purified to 4.74 fold with 55. 38 U/mg of specific activity with the yield of 28.92%. The molecular weight of the purified bacteriocin was estimated as 21 kDa. The purified bacteriocin exhibited complete inactivation of antimicrobial activity when treated with proteinase K, pronase, chymotrypsin, trypsin, pepsin and papain. The purified bacteriocin exhibited broad inhibitory spectrum against both Gram positive and negative bacteria. Conclusions: It is concluded that the ability of bacteriocin in inhibiting a wide-range of pathogenic bacteria is of potential interest for food safety and may have future applications in food preservative. |
format |
Article in Journal/Newspaper |
author |
Sivaramasamy Elayaraja Neelamegam Annamalai Packiyam Mayavu Thangavel Balasubramanian |
author_facet |
Sivaramasamy Elayaraja Neelamegam Annamalai Packiyam Mayavu Thangavel Balasubramanian |
author_sort |
Sivaramasamy Elayaraja |
title |
Production, purification and characterization of bacteriocin from Lactobacillus murinus AU06 and its broad antibacterial spectrum |
title_short |
Production, purification and characterization of bacteriocin from Lactobacillus murinus AU06 and its broad antibacterial spectrum |
title_full |
Production, purification and characterization of bacteriocin from Lactobacillus murinus AU06 and its broad antibacterial spectrum |
title_fullStr |
Production, purification and characterization of bacteriocin from Lactobacillus murinus AU06 and its broad antibacterial spectrum |
title_full_unstemmed |
Production, purification and characterization of bacteriocin from Lactobacillus murinus AU06 and its broad antibacterial spectrum |
title_sort |
production, purification and characterization of bacteriocin from lactobacillus murinus au06 and its broad antibacterial spectrum |
publisher |
Wolters Kluwer Medknow Publications |
publishDate |
2014 |
url |
https://doi.org/10.12980/APJTB.4.2014C537 https://doaj.org/article/d36cd2ddcfd1465884cc7c6e4a595cfd |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Asian Pacific Journal of Tropical Biomedicine, Vol 4, Iss S1, Pp S305-S311 (2014) |
op_relation |
http://www.sciencedirect.com/science/article/pii/S2221169115302872 https://doaj.org/toc/2221-1691 2221-1691 doi:10.12980/APJTB.4.2014C537 https://doaj.org/article/d36cd2ddcfd1465884cc7c6e4a595cfd |
op_doi |
https://doi.org/10.12980/APJTB.4.2014C537 |
container_title |
Asian Pacific Journal of Tropical Biomedicine |
container_volume |
4 |
container_start_page |
S305 |
op_container_end_page |
S311 |
_version_ |
1766336949614804992 |