Phototrophic pigment diversity and picophytoplankton in permafrost thaw lakes
Permafrost thaw lakes (thermokarst lakes) are widely distributed across the northern landscape, and are known to be biogeochemically active sites that emit large amounts of carbon to the atmosphere as CH 4 and CO 2 . However, the abundance and composition of the photosynthetic communities that fix C...
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ftdoajarticles:oai:doaj.org/article:d35ea4a559a14147b82eecea5dd4f16f 2023-05-15T17:54:29+02:00 Phototrophic pigment diversity and picophytoplankton in permafrost thaw lakes A. Przytulska J. Comte S. Crevecoeur C. Lovejoy I. Laurion W. F. Vincent 2016-01-01T00:00:00Z https://doi.org/10.5194/bg-13-13-2016 https://doaj.org/article/d35ea4a559a14147b82eecea5dd4f16f EN eng Copernicus Publications http://www.biogeosciences.net/13/13/2016/bg-13-13-2016.pdf https://doaj.org/toc/1726-4170 https://doaj.org/toc/1726-4189 1726-4170 1726-4189 doi:10.5194/bg-13-13-2016 https://doaj.org/article/d35ea4a559a14147b82eecea5dd4f16f Biogeosciences, Vol 13, Iss 1, Pp 13-26 (2016) Ecology QH540-549.5 Life QH501-531 Geology QE1-996.5 article 2016 ftdoajarticles https://doi.org/10.5194/bg-13-13-2016 2022-12-31T05:35:07Z Permafrost thaw lakes (thermokarst lakes) are widely distributed across the northern landscape, and are known to be biogeochemically active sites that emit large amounts of carbon to the atmosphere as CH 4 and CO 2 . However, the abundance and composition of the photosynthetic communities that fix CO 2 have been little explored in this ecosystem type. In order to identify the major groups of phototrophic organisms and their controlling variables, we sampled 12 permafrost thaw lakes along a permafrost degradation gradient in northern Québec, Canada. Additional samples were taken from five rock-basin reference lakes in the region to determine if the thaw lakes differed in limnological properties and phototrophs. Phytoplankton community structure was determined by high-performance liquid chromatography analysis of their photoprotective and photosynthetic pigments, and autotrophic picoplankton concentrations were assessed by flow cytometry. One of the black-colored lakes located in a landscape of rapidly degrading palsas (permafrost mounds) was selected for high-throughput 18S rRNA sequencing to complement conclusions based on the pigment and cytometry analyses. The results showed that the limnological properties of the thaw lakes differed significantly from the reference lakes, and were more highly stratified. However, both waterbody types contained similarly diverse phytoplankton groups, with dominance of the pigment assemblages by fucoxanthin-containing taxa, as well as chlorophytes, cryptophytes and cyanobacteria. Chlorophyll a concentrations (Chl a ) were correlated with total phosphorus (TP), and both were significantly higher in the thaw lakes (overall means of 3.3 µg Chl a L −1 and 34 µg TP L −1 ) relative to the reference lakes (2.0 µg Chl a L −1 and 8.2 µg TP L −1 ). Stepwise multiple regression of Chl a against the other algal pigments showed that it was largely a function of alloxanthin, fucoxanthin and Chl b ( R 2 = 0.85). The bottom waters of two of the thaw lakes also contained high concentrations of ... Article in Journal/Newspaper palsas permafrost Thermokarst Directory of Open Access Journals: DOAJ Articles Canada Biogeosciences 13 1 13 26 |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Ecology QH540-549.5 Life QH501-531 Geology QE1-996.5 |
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Ecology QH540-549.5 Life QH501-531 Geology QE1-996.5 A. Przytulska J. Comte S. Crevecoeur C. Lovejoy I. Laurion W. F. Vincent Phototrophic pigment diversity and picophytoplankton in permafrost thaw lakes |
topic_facet |
Ecology QH540-549.5 Life QH501-531 Geology QE1-996.5 |
description |
Permafrost thaw lakes (thermokarst lakes) are widely distributed across the northern landscape, and are known to be biogeochemically active sites that emit large amounts of carbon to the atmosphere as CH 4 and CO 2 . However, the abundance and composition of the photosynthetic communities that fix CO 2 have been little explored in this ecosystem type. In order to identify the major groups of phototrophic organisms and their controlling variables, we sampled 12 permafrost thaw lakes along a permafrost degradation gradient in northern Québec, Canada. Additional samples were taken from five rock-basin reference lakes in the region to determine if the thaw lakes differed in limnological properties and phototrophs. Phytoplankton community structure was determined by high-performance liquid chromatography analysis of their photoprotective and photosynthetic pigments, and autotrophic picoplankton concentrations were assessed by flow cytometry. One of the black-colored lakes located in a landscape of rapidly degrading palsas (permafrost mounds) was selected for high-throughput 18S rRNA sequencing to complement conclusions based on the pigment and cytometry analyses. The results showed that the limnological properties of the thaw lakes differed significantly from the reference lakes, and were more highly stratified. However, both waterbody types contained similarly diverse phytoplankton groups, with dominance of the pigment assemblages by fucoxanthin-containing taxa, as well as chlorophytes, cryptophytes and cyanobacteria. Chlorophyll a concentrations (Chl a ) were correlated with total phosphorus (TP), and both were significantly higher in the thaw lakes (overall means of 3.3 µg Chl a L −1 and 34 µg TP L −1 ) relative to the reference lakes (2.0 µg Chl a L −1 and 8.2 µg TP L −1 ). Stepwise multiple regression of Chl a against the other algal pigments showed that it was largely a function of alloxanthin, fucoxanthin and Chl b ( R 2 = 0.85). The bottom waters of two of the thaw lakes also contained high concentrations of ... |
format |
Article in Journal/Newspaper |
author |
A. Przytulska J. Comte S. Crevecoeur C. Lovejoy I. Laurion W. F. Vincent |
author_facet |
A. Przytulska J. Comte S. Crevecoeur C. Lovejoy I. Laurion W. F. Vincent |
author_sort |
A. Przytulska |
title |
Phototrophic pigment diversity and picophytoplankton in permafrost thaw lakes |
title_short |
Phototrophic pigment diversity and picophytoplankton in permafrost thaw lakes |
title_full |
Phototrophic pigment diversity and picophytoplankton in permafrost thaw lakes |
title_fullStr |
Phototrophic pigment diversity and picophytoplankton in permafrost thaw lakes |
title_full_unstemmed |
Phototrophic pigment diversity and picophytoplankton in permafrost thaw lakes |
title_sort |
phototrophic pigment diversity and picophytoplankton in permafrost thaw lakes |
publisher |
Copernicus Publications |
publishDate |
2016 |
url |
https://doi.org/10.5194/bg-13-13-2016 https://doaj.org/article/d35ea4a559a14147b82eecea5dd4f16f |
geographic |
Canada |
geographic_facet |
Canada |
genre |
palsas permafrost Thermokarst |
genre_facet |
palsas permafrost Thermokarst |
op_source |
Biogeosciences, Vol 13, Iss 1, Pp 13-26 (2016) |
op_relation |
http://www.biogeosciences.net/13/13/2016/bg-13-13-2016.pdf https://doaj.org/toc/1726-4170 https://doaj.org/toc/1726-4189 1726-4170 1726-4189 doi:10.5194/bg-13-13-2016 https://doaj.org/article/d35ea4a559a14147b82eecea5dd4f16f |
op_doi |
https://doi.org/10.5194/bg-13-13-2016 |
container_title |
Biogeosciences |
container_volume |
13 |
container_issue |
1 |
container_start_page |
13 |
op_container_end_page |
26 |
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1766162248218181632 |