Desiccation survival in an Antarctic nematode: molecular analysis using expressed sequenced tags
Abstract Background Nematodes are the dominant soil animals in Antarctic Dry Valleys and are capable of surviving desiccation and freezing in an anhydrobiotic state. Genes induced by desiccation stress have been successfully enumerated in nematodes; however we have little knowledge of gene regulatio...
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ftdoajarticles:oai:doaj.org/article:d331c5de65f44db08860060fa76ec3c1 2023-05-15T13:53:05+02:00 Desiccation survival in an Antarctic nematode: molecular analysis using expressed sequenced tags Wall Diana H Adhikari Bishwo N Adams Byron J 2009-02-01T00:00:00Z https://doi.org/10.1186/1471-2164-10-69 https://doaj.org/article/d331c5de65f44db08860060fa76ec3c1 EN eng BMC http://www.biomedcentral.com/1471-2164/10/69 https://doaj.org/toc/1471-2164 doi:10.1186/1471-2164-10-69 1471-2164 https://doaj.org/article/d331c5de65f44db08860060fa76ec3c1 BMC Genomics, Vol 10, Iss 1, p 69 (2009) Biotechnology TP248.13-248.65 Genetics QH426-470 article 2009 ftdoajarticles https://doi.org/10.1186/1471-2164-10-69 2022-12-30T22:29:16Z Abstract Background Nematodes are the dominant soil animals in Antarctic Dry Valleys and are capable of surviving desiccation and freezing in an anhydrobiotic state. Genes induced by desiccation stress have been successfully enumerated in nematodes; however we have little knowledge of gene regulation by Antarctic nematodes which can survive multiple environmental stresses. To address this problem we investigated the genetic responses of a nematode species, Plectus murrayi , that is capable of tolerating Antarctic environmental extremes, in particular desiccation and freezing. In this study, we provide the first insight into the desiccation induced transcriptome of an Antarctic nematode through cDNA library construction and suppressive subtractive hybridization. Results We obtained 2,486 expressed sequence tags (ESTs) from 2,586 clones derived from the cDNA library of desiccated P. murrayi . The 2,486 ESTs formed 1,387 putative unique transcripts of which 523 (38%) had matches in the model-nematode Caenorhabditis elegans , 107 (7%) in nematodes other than C. elegans , 153 (11%) in non-nematode organisms and 605 (44%) had no significant match to any sequences in the current databases. The 1,387 unique transcripts were functionally classified by using Gene Ontology (GO) hierarchy and the Kyoto Encyclopedia of Genes and Genomes (KEGG) database. The results indicate that the transcriptome contains a group of transcripts from diverse functional areas. The subtractive library of desiccated nematodes showed 80 transcripts differentially expressed during desiccation stress, of which 28% were metabolism related, 19% were involved in environmental information processing, 28% involved in genetic information processing and 21% were novel transcripts. Expression profiling of 14 selected genes by quantitative Real-time PCR showed 9 genes significantly up-regulated, 3 down-regulated and 2 continuously expressed in response to desiccation. Conclusion The establishment of a desiccation EST collection for Plectus murrayi , a ... Article in Journal/Newspaper Antarc* Antarctic Directory of Open Access Journals: DOAJ Articles Antarctic BMC Genomics 10 1 69 |
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Biotechnology TP248.13-248.65 Genetics QH426-470 |
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Biotechnology TP248.13-248.65 Genetics QH426-470 Wall Diana H Adhikari Bishwo N Adams Byron J Desiccation survival in an Antarctic nematode: molecular analysis using expressed sequenced tags |
topic_facet |
Biotechnology TP248.13-248.65 Genetics QH426-470 |
description |
Abstract Background Nematodes are the dominant soil animals in Antarctic Dry Valleys and are capable of surviving desiccation and freezing in an anhydrobiotic state. Genes induced by desiccation stress have been successfully enumerated in nematodes; however we have little knowledge of gene regulation by Antarctic nematodes which can survive multiple environmental stresses. To address this problem we investigated the genetic responses of a nematode species, Plectus murrayi , that is capable of tolerating Antarctic environmental extremes, in particular desiccation and freezing. In this study, we provide the first insight into the desiccation induced transcriptome of an Antarctic nematode through cDNA library construction and suppressive subtractive hybridization. Results We obtained 2,486 expressed sequence tags (ESTs) from 2,586 clones derived from the cDNA library of desiccated P. murrayi . The 2,486 ESTs formed 1,387 putative unique transcripts of which 523 (38%) had matches in the model-nematode Caenorhabditis elegans , 107 (7%) in nematodes other than C. elegans , 153 (11%) in non-nematode organisms and 605 (44%) had no significant match to any sequences in the current databases. The 1,387 unique transcripts were functionally classified by using Gene Ontology (GO) hierarchy and the Kyoto Encyclopedia of Genes and Genomes (KEGG) database. The results indicate that the transcriptome contains a group of transcripts from diverse functional areas. The subtractive library of desiccated nematodes showed 80 transcripts differentially expressed during desiccation stress, of which 28% were metabolism related, 19% were involved in environmental information processing, 28% involved in genetic information processing and 21% were novel transcripts. Expression profiling of 14 selected genes by quantitative Real-time PCR showed 9 genes significantly up-regulated, 3 down-regulated and 2 continuously expressed in response to desiccation. Conclusion The establishment of a desiccation EST collection for Plectus murrayi , a ... |
format |
Article in Journal/Newspaper |
author |
Wall Diana H Adhikari Bishwo N Adams Byron J |
author_facet |
Wall Diana H Adhikari Bishwo N Adams Byron J |
author_sort |
Wall Diana H |
title |
Desiccation survival in an Antarctic nematode: molecular analysis using expressed sequenced tags |
title_short |
Desiccation survival in an Antarctic nematode: molecular analysis using expressed sequenced tags |
title_full |
Desiccation survival in an Antarctic nematode: molecular analysis using expressed sequenced tags |
title_fullStr |
Desiccation survival in an Antarctic nematode: molecular analysis using expressed sequenced tags |
title_full_unstemmed |
Desiccation survival in an Antarctic nematode: molecular analysis using expressed sequenced tags |
title_sort |
desiccation survival in an antarctic nematode: molecular analysis using expressed sequenced tags |
publisher |
BMC |
publishDate |
2009 |
url |
https://doi.org/10.1186/1471-2164-10-69 https://doaj.org/article/d331c5de65f44db08860060fa76ec3c1 |
geographic |
Antarctic |
geographic_facet |
Antarctic |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_source |
BMC Genomics, Vol 10, Iss 1, p 69 (2009) |
op_relation |
http://www.biomedcentral.com/1471-2164/10/69 https://doaj.org/toc/1471-2164 doi:10.1186/1471-2164-10-69 1471-2164 https://doaj.org/article/d331c5de65f44db08860060fa76ec3c1 |
op_doi |
https://doi.org/10.1186/1471-2164-10-69 |
container_title |
BMC Genomics |
container_volume |
10 |
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1 |
container_start_page |
69 |
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1766258035966083072 |