Polysaccharides from the Chinese medicinal herb Achyranthes bidentata enhance anti-malarial immunity during Plasmodium yoelii 17XL infection in mice
Abstract Background Clinical immunity to malaria in human populations is developed after repeated exposure to malaria. Regulation and balance of host immune responses may lead to optimal immunity against malaria parasite infection. Polysaccharides (ABPS) derived from the Chinese herb ox knee Achyran...
Published in: | Malaria Journal |
---|---|
Main Authors: | , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
BMC
2012
|
Subjects: | |
Online Access: | https://doi.org/10.1186/1475-2875-11-49 https://doaj.org/article/d325735975e8422f9a2aa75d9d4aa028 |
id |
ftdoajarticles:oai:doaj.org/article:d325735975e8422f9a2aa75d9d4aa028 |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:d325735975e8422f9a2aa75d9d4aa028 2023-05-15T15:15:56+02:00 Polysaccharides from the Chinese medicinal herb Achyranthes bidentata enhance anti-malarial immunity during Plasmodium yoelii 17XL infection in mice Zhu Xiaotong Pan Yanyan Zheng Li Cui Liwang Cao Yaming 2012-02-01T00:00:00Z https://doi.org/10.1186/1475-2875-11-49 https://doaj.org/article/d325735975e8422f9a2aa75d9d4aa028 EN eng BMC http://www.malariajournal.com/content/11/1/49 https://doaj.org/toc/1475-2875 doi:10.1186/1475-2875-11-49 1475-2875 https://doaj.org/article/d325735975e8422f9a2aa75d9d4aa028 Malaria Journal, Vol 11, Iss 1, p 49 (2012) Achyranthes bidentata polysaccharides Plasmodium yoelii 17XL Immune responses Immuno modulatory effect Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2012 ftdoajarticles https://doi.org/10.1186/1475-2875-11-49 2022-12-31T00:28:56Z Abstract Background Clinical immunity to malaria in human populations is developed after repeated exposure to malaria. Regulation and balance of host immune responses may lead to optimal immunity against malaria parasite infection. Polysaccharides (ABPS) derived from the Chinese herb ox knee Achyranthes bidentata possess immuno-modulatory functions. The aim of this study is to use the rodent malaria model Plasmodium yoelii 17XL ( P. y 17XL) to examine whether pretreatment with ABPS will modulate host immunity against malaria infection and improve the outcome of the disease. Methods To determine whether ABPS could modulate immunity against malaria, mice were pretreated with ABPS prior to blood-stage infection by P. y 17XL. Host survival and parasitaemia were monitored daily. The effect of pretreatment on host immune responses was studied through the quantitation of cytokines, dendritic cell populations, and natural regulatory T cells (Treg). Results Pretreatment with ABPS prior to infection significantly extended the survival time of mice after P. y 17XL infection. At three and five days post-infection, ABPS pretreated mice developed stronger Th1 immune responses against malaria infection with the number of F4/80 + CD36 + macrophages and levels of IFN-γ, TNF-α and nitric oxide being significantly higher than in the control group. More importantly, ABPS-treated mice developed more myeloid (CD11c + CD11b + ) and plasmacytoid dendritic cells (CD11c + CD45R + /B220 + ) than control mice. ABPS pretreatment also resulted in modulated expression of MHC-II, CD86, and especially Toll-like receptor 9 by CD11c + dendritic cells. In comparison, pretreatment with ABPS did not alter the number of natural Treg or the production of the anti-inflammatory cytokine IL-10. Conclusion Pretreatment with the immuno-modulatory ABPS selectively enhanced Th1 immune responses to control the proliferation of malaria parasites, and prolonged the survival of mice during subsequent malaria infection. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Malaria Journal 11 1 49 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Achyranthes bidentata polysaccharides Plasmodium yoelii 17XL Immune responses Immuno modulatory effect Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
spellingShingle |
Achyranthes bidentata polysaccharides Plasmodium yoelii 17XL Immune responses Immuno modulatory effect Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Zhu Xiaotong Pan Yanyan Zheng Li Cui Liwang Cao Yaming Polysaccharides from the Chinese medicinal herb Achyranthes bidentata enhance anti-malarial immunity during Plasmodium yoelii 17XL infection in mice |
topic_facet |
Achyranthes bidentata polysaccharides Plasmodium yoelii 17XL Immune responses Immuno modulatory effect Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Abstract Background Clinical immunity to malaria in human populations is developed after repeated exposure to malaria. Regulation and balance of host immune responses may lead to optimal immunity against malaria parasite infection. Polysaccharides (ABPS) derived from the Chinese herb ox knee Achyranthes bidentata possess immuno-modulatory functions. The aim of this study is to use the rodent malaria model Plasmodium yoelii 17XL ( P. y 17XL) to examine whether pretreatment with ABPS will modulate host immunity against malaria infection and improve the outcome of the disease. Methods To determine whether ABPS could modulate immunity against malaria, mice were pretreated with ABPS prior to blood-stage infection by P. y 17XL. Host survival and parasitaemia were monitored daily. The effect of pretreatment on host immune responses was studied through the quantitation of cytokines, dendritic cell populations, and natural regulatory T cells (Treg). Results Pretreatment with ABPS prior to infection significantly extended the survival time of mice after P. y 17XL infection. At three and five days post-infection, ABPS pretreated mice developed stronger Th1 immune responses against malaria infection with the number of F4/80 + CD36 + macrophages and levels of IFN-γ, TNF-α and nitric oxide being significantly higher than in the control group. More importantly, ABPS-treated mice developed more myeloid (CD11c + CD11b + ) and plasmacytoid dendritic cells (CD11c + CD45R + /B220 + ) than control mice. ABPS pretreatment also resulted in modulated expression of MHC-II, CD86, and especially Toll-like receptor 9 by CD11c + dendritic cells. In comparison, pretreatment with ABPS did not alter the number of natural Treg or the production of the anti-inflammatory cytokine IL-10. Conclusion Pretreatment with the immuno-modulatory ABPS selectively enhanced Th1 immune responses to control the proliferation of malaria parasites, and prolonged the survival of mice during subsequent malaria infection. |
format |
Article in Journal/Newspaper |
author |
Zhu Xiaotong Pan Yanyan Zheng Li Cui Liwang Cao Yaming |
author_facet |
Zhu Xiaotong Pan Yanyan Zheng Li Cui Liwang Cao Yaming |
author_sort |
Zhu Xiaotong |
title |
Polysaccharides from the Chinese medicinal herb Achyranthes bidentata enhance anti-malarial immunity during Plasmodium yoelii 17XL infection in mice |
title_short |
Polysaccharides from the Chinese medicinal herb Achyranthes bidentata enhance anti-malarial immunity during Plasmodium yoelii 17XL infection in mice |
title_full |
Polysaccharides from the Chinese medicinal herb Achyranthes bidentata enhance anti-malarial immunity during Plasmodium yoelii 17XL infection in mice |
title_fullStr |
Polysaccharides from the Chinese medicinal herb Achyranthes bidentata enhance anti-malarial immunity during Plasmodium yoelii 17XL infection in mice |
title_full_unstemmed |
Polysaccharides from the Chinese medicinal herb Achyranthes bidentata enhance anti-malarial immunity during Plasmodium yoelii 17XL infection in mice |
title_sort |
polysaccharides from the chinese medicinal herb achyranthes bidentata enhance anti-malarial immunity during plasmodium yoelii 17xl infection in mice |
publisher |
BMC |
publishDate |
2012 |
url |
https://doi.org/10.1186/1475-2875-11-49 https://doaj.org/article/d325735975e8422f9a2aa75d9d4aa028 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Malaria Journal, Vol 11, Iss 1, p 49 (2012) |
op_relation |
http://www.malariajournal.com/content/11/1/49 https://doaj.org/toc/1475-2875 doi:10.1186/1475-2875-11-49 1475-2875 https://doaj.org/article/d325735975e8422f9a2aa75d9d4aa028 |
op_doi |
https://doi.org/10.1186/1475-2875-11-49 |
container_title |
Malaria Journal |
container_volume |
11 |
container_issue |
1 |
container_start_page |
49 |
_version_ |
1766346266449543168 |