Detection of Leishmania (V) guyanensis in Rhipicephalus (Boophilus) microplus (Acari: Ixodidae) collected from Pecari tajacu
Introduction: Previous studies identified the presence of Leishmania infantum in Rhipicephalus sanguineus and indicated the possibility that it could transmit leishmaniasis to a variety of hosts. Objective: To identify parasites of Leishmania (Viannia) spp. in ticks collected from wild animals in an...
Published in: | Biomédica |
---|---|
Main Authors: | , , , |
Format: | Article in Journal/Newspaper |
Language: | English Spanish |
Published: |
Instituto Nacional de Salud
2017
|
Subjects: | |
Online Access: | https://doi.org/10.7705/biomedica.v37i0.3435 https://doaj.org/article/d2f5b24d0f2d47c2b1e3bb9bd2d56be1 |
id |
ftdoajarticles:oai:doaj.org/article:d2f5b24d0f2d47c2b1e3bb9bd2d56be1 |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:d2f5b24d0f2d47c2b1e3bb9bd2d56be1 2023-05-15T15:06:03+02:00 Detection of Leishmania (V) guyanensis in Rhipicephalus (Boophilus) microplus (Acari: Ixodidae) collected from Pecari tajacu Jesús E. Rojas-Jaimes Germán H. Correa-Nuñez Nyshon Rojas Omar Cáceres-Rey 2017-03-01T00:00:00Z https://doi.org/10.7705/biomedica.v37i0.3435 https://doaj.org/article/d2f5b24d0f2d47c2b1e3bb9bd2d56be1 EN ES eng spa Instituto Nacional de Salud https://www.revistabiomedica.org/index.php/biomedica/article/view/3435 https://doaj.org/toc/0120-4157 0120-4157 doi:10.7705/biomedica.v37i0.3435 https://doaj.org/article/d2f5b24d0f2d47c2b1e3bb9bd2d56be1 Biomédica: revista del Instituto Nacional de Salud, Vol 37, Iss 0, Pp 208-214 (2017) Leishmania guyanensis leishmaniasis Rhipicephalus artiodactyla Peru Medicine R Arctic medicine. Tropical medicine RC955-962 article 2017 ftdoajarticles https://doi.org/10.7705/biomedica.v37i0.3435 2022-12-31T02:08:54Z Introduction: Previous studies identified the presence of Leishmania infantum in Rhipicephalus sanguineus and indicated the possibility that it could transmit leishmaniasis to a variety of hosts. Objective: To identify parasites of Leishmania (Viannia) spp. in ticks collected from wild animals in an endemic area for leishmaniasis. Materials and methods: We performed 81 individual DNA extractions from ticks collected from three Tapirus terrestris and three Pecari tajacu in Madre de Dios, Perú. Ticks were taxonomically identified and they were subsequently prepared to identify Leishmania (Viannia) spp. kDNA by PCR and the species of Leishmania by HRM-PCR. Results: Leishmania (Viannia) kDNA was detected in three wild ticks of the species R. microplus, collected from a collard peccary (P. tajacu) hunted in the forests of Madre de Dios. The HRM-PCR showed that one of the positive samples had a kDNA curve compatible with L. (V) guyanensis. Conclusion: The results showed the presence of L. (V) guyanensis DNA in R. microplus possibly acquired after biting a collarde peccary. Therefore, it is important to design future studies to clarify R. microplus involvement in the transmission of leishmaniasis. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Collard ENVELOPE(31.117,31.117,-72.633,-72.633) Biomédica 37 208 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English Spanish |
topic |
Leishmania guyanensis leishmaniasis Rhipicephalus artiodactyla Peru Medicine R Arctic medicine. Tropical medicine RC955-962 |
spellingShingle |
Leishmania guyanensis leishmaniasis Rhipicephalus artiodactyla Peru Medicine R Arctic medicine. Tropical medicine RC955-962 Jesús E. Rojas-Jaimes Germán H. Correa-Nuñez Nyshon Rojas Omar Cáceres-Rey Detection of Leishmania (V) guyanensis in Rhipicephalus (Boophilus) microplus (Acari: Ixodidae) collected from Pecari tajacu |
topic_facet |
Leishmania guyanensis leishmaniasis Rhipicephalus artiodactyla Peru Medicine R Arctic medicine. Tropical medicine RC955-962 |
description |
Introduction: Previous studies identified the presence of Leishmania infantum in Rhipicephalus sanguineus and indicated the possibility that it could transmit leishmaniasis to a variety of hosts. Objective: To identify parasites of Leishmania (Viannia) spp. in ticks collected from wild animals in an endemic area for leishmaniasis. Materials and methods: We performed 81 individual DNA extractions from ticks collected from three Tapirus terrestris and three Pecari tajacu in Madre de Dios, Perú. Ticks were taxonomically identified and they were subsequently prepared to identify Leishmania (Viannia) spp. kDNA by PCR and the species of Leishmania by HRM-PCR. Results: Leishmania (Viannia) kDNA was detected in three wild ticks of the species R. microplus, collected from a collard peccary (P. tajacu) hunted in the forests of Madre de Dios. The HRM-PCR showed that one of the positive samples had a kDNA curve compatible with L. (V) guyanensis. Conclusion: The results showed the presence of L. (V) guyanensis DNA in R. microplus possibly acquired after biting a collarde peccary. Therefore, it is important to design future studies to clarify R. microplus involvement in the transmission of leishmaniasis. |
format |
Article in Journal/Newspaper |
author |
Jesús E. Rojas-Jaimes Germán H. Correa-Nuñez Nyshon Rojas Omar Cáceres-Rey |
author_facet |
Jesús E. Rojas-Jaimes Germán H. Correa-Nuñez Nyshon Rojas Omar Cáceres-Rey |
author_sort |
Jesús E. Rojas-Jaimes |
title |
Detection of Leishmania (V) guyanensis in Rhipicephalus (Boophilus) microplus (Acari: Ixodidae) collected from Pecari tajacu |
title_short |
Detection of Leishmania (V) guyanensis in Rhipicephalus (Boophilus) microplus (Acari: Ixodidae) collected from Pecari tajacu |
title_full |
Detection of Leishmania (V) guyanensis in Rhipicephalus (Boophilus) microplus (Acari: Ixodidae) collected from Pecari tajacu |
title_fullStr |
Detection of Leishmania (V) guyanensis in Rhipicephalus (Boophilus) microplus (Acari: Ixodidae) collected from Pecari tajacu |
title_full_unstemmed |
Detection of Leishmania (V) guyanensis in Rhipicephalus (Boophilus) microplus (Acari: Ixodidae) collected from Pecari tajacu |
title_sort |
detection of leishmania (v) guyanensis in rhipicephalus (boophilus) microplus (acari: ixodidae) collected from pecari tajacu |
publisher |
Instituto Nacional de Salud |
publishDate |
2017 |
url |
https://doi.org/10.7705/biomedica.v37i0.3435 https://doaj.org/article/d2f5b24d0f2d47c2b1e3bb9bd2d56be1 |
long_lat |
ENVELOPE(31.117,31.117,-72.633,-72.633) |
geographic |
Arctic Collard |
geographic_facet |
Arctic Collard |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Biomédica: revista del Instituto Nacional de Salud, Vol 37, Iss 0, Pp 208-214 (2017) |
op_relation |
https://www.revistabiomedica.org/index.php/biomedica/article/view/3435 https://doaj.org/toc/0120-4157 0120-4157 doi:10.7705/biomedica.v37i0.3435 https://doaj.org/article/d2f5b24d0f2d47c2b1e3bb9bd2d56be1 |
op_doi |
https://doi.org/10.7705/biomedica.v37i0.3435 |
container_title |
Biomédica |
container_volume |
37 |
container_start_page |
208 |
_version_ |
1766337706473816064 |