Febrile temperatures increase in vitro antibody affinity for malarial and dengue antigens.
Fever is a regulated increase of the body temperature resulting from both infectious and non-infectious causes. Fever is known to play a role in modulating immune responses to infection, but the potential of febrile temperatures in regulating antigen binding affinity to antibodies has not been explo...
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ftdoajarticles:oai:doaj.org/article:d28a145eb7514548be282ba0cc7e40c2 2023-05-15T15:04:17+02:00 Febrile temperatures increase in vitro antibody affinity for malarial and dengue antigens. Razvan C Stan Katia S Françoso Rubens P S Alves Luís Carlos S Ferreira Irene S Soares Maristela M de Camargo 2019-04-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0007239 https://doaj.org/article/d28a145eb7514548be282ba0cc7e40c2 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC6464238?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0007239 https://doaj.org/article/d28a145eb7514548be282ba0cc7e40c2 PLoS Neglected Tropical Diseases, Vol 13, Iss 4, p e0007239 (2019) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2019 ftdoajarticles https://doi.org/10.1371/journal.pntd.0007239 2022-12-30T21:54:52Z Fever is a regulated increase of the body temperature resulting from both infectious and non-infectious causes. Fever is known to play a role in modulating immune responses to infection, but the potential of febrile temperatures in regulating antigen binding affinity to antibodies has not been explored. Here we investigated this process under in vitro conditions using Isothermal titration calorimetry and ELISA. We used selected malarial and dengue antigens against specific monoclonal antibodies, and observed a marked increase in the affinity of these antibody-antigen complexes at 40°C, compared to physiological (37°C) or pathophysiological temperatures (42°C). Induced thermal equilibration of the protein partners at these temperatures in vitro, prior to measurements, further increased their binding affinity. These results suggest another positive and adaptive role for fever in vivo, and highlight the favourable role of thermal priming in enhancing protein-protein affinity for samples with limited availability. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 13 4 e0007239 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
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English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Razvan C Stan Katia S Françoso Rubens P S Alves Luís Carlos S Ferreira Irene S Soares Maristela M de Camargo Febrile temperatures increase in vitro antibody affinity for malarial and dengue antigens. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Fever is a regulated increase of the body temperature resulting from both infectious and non-infectious causes. Fever is known to play a role in modulating immune responses to infection, but the potential of febrile temperatures in regulating antigen binding affinity to antibodies has not been explored. Here we investigated this process under in vitro conditions using Isothermal titration calorimetry and ELISA. We used selected malarial and dengue antigens against specific monoclonal antibodies, and observed a marked increase in the affinity of these antibody-antigen complexes at 40°C, compared to physiological (37°C) or pathophysiological temperatures (42°C). Induced thermal equilibration of the protein partners at these temperatures in vitro, prior to measurements, further increased their binding affinity. These results suggest another positive and adaptive role for fever in vivo, and highlight the favourable role of thermal priming in enhancing protein-protein affinity for samples with limited availability. |
format |
Article in Journal/Newspaper |
author |
Razvan C Stan Katia S Françoso Rubens P S Alves Luís Carlos S Ferreira Irene S Soares Maristela M de Camargo |
author_facet |
Razvan C Stan Katia S Françoso Rubens P S Alves Luís Carlos S Ferreira Irene S Soares Maristela M de Camargo |
author_sort |
Razvan C Stan |
title |
Febrile temperatures increase in vitro antibody affinity for malarial and dengue antigens. |
title_short |
Febrile temperatures increase in vitro antibody affinity for malarial and dengue antigens. |
title_full |
Febrile temperatures increase in vitro antibody affinity for malarial and dengue antigens. |
title_fullStr |
Febrile temperatures increase in vitro antibody affinity for malarial and dengue antigens. |
title_full_unstemmed |
Febrile temperatures increase in vitro antibody affinity for malarial and dengue antigens. |
title_sort |
febrile temperatures increase in vitro antibody affinity for malarial and dengue antigens. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2019 |
url |
https://doi.org/10.1371/journal.pntd.0007239 https://doaj.org/article/d28a145eb7514548be282ba0cc7e40c2 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 13, Iss 4, p e0007239 (2019) |
op_relation |
http://europepmc.org/articles/PMC6464238?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0007239 https://doaj.org/article/d28a145eb7514548be282ba0cc7e40c2 |
op_doi |
https://doi.org/10.1371/journal.pntd.0007239 |
container_title |
PLOS Neglected Tropical Diseases |
container_volume |
13 |
container_issue |
4 |
container_start_page |
e0007239 |
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1766336081557454848 |