The genus Madurella: Molecular identification and epidemiology in Sudan.
Eumycetoma (mycotic mycetoma) is the fungal form of mycetoma, a subcutaneous infection occurring in individuals living in endemic areas of the disease. The Sudan is hyperendemic for mycetoma, with the highest incidence being reported from Gezira State, Central Sudan. The present study was conducted...
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ftdoajarticles:oai:doaj.org/article:d27544a1884d44488c8ca1f72d31371f 2023-05-15T15:06:35+02:00 The genus Madurella: Molecular identification and epidemiology in Sudan. Elhadi A Ahmed Bakri Y M Nour Adam D Abakar Samirah Hamid Ahmed A Mohamadani Mohamed Daffalla Mogahid Mahmoud Hisham N Altayb Marie Desnos-Ollivier Sybren de Hoog Sarah A Ahmed 2020-07-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0008420 https://doaj.org/article/d27544a1884d44488c8ca1f72d31371f EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pntd.0008420 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0008420 https://doaj.org/article/d27544a1884d44488c8ca1f72d31371f PLoS Neglected Tropical Diseases, Vol 14, Iss 7, p e0008420 (2020) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2020 ftdoajarticles https://doi.org/10.1371/journal.pntd.0008420 2022-12-31T07:17:08Z Eumycetoma (mycotic mycetoma) is the fungal form of mycetoma, a subcutaneous infection occurring in individuals living in endemic areas of the disease. The Sudan is hyperendemic for mycetoma, with the highest incidence being reported from Gezira State, Central Sudan. The present study was conducted at the Gezira Mycetoma Center and aimed to determine the cause of black-grain eumycetoma in the state and describe its epidemiology. Black-grain specimens were collected during the surgical operation and direct detection of the causative agent was performed using M. mycetomatis species-specific PCR and ITS PCR followed by sequencing. Black-grain was reported from 93.3% of all confirmed mycetoma cases (n = 111/119), with a prevalence in young males. Of the 91 samples subjected to direct PCR, 90.1% (n = 82) gave positive results. The predominant species (88.2%) was Madurella mycetomatis. One sample was identified as M. fahalii, one as M. tropicana, and one matched the phytopathogenic species Sphaerulina rhododendricola. The highest endemic zones were Southern Gezira (76.6%) and Northern Sinnar (23.4%). The study confirmed that direct molecular detection on grains provides rapid and specific diagnosis of agents of eumycetoma. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 14 7 e0008420 |
institution |
Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Elhadi A Ahmed Bakri Y M Nour Adam D Abakar Samirah Hamid Ahmed A Mohamadani Mohamed Daffalla Mogahid Mahmoud Hisham N Altayb Marie Desnos-Ollivier Sybren de Hoog Sarah A Ahmed The genus Madurella: Molecular identification and epidemiology in Sudan. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Eumycetoma (mycotic mycetoma) is the fungal form of mycetoma, a subcutaneous infection occurring in individuals living in endemic areas of the disease. The Sudan is hyperendemic for mycetoma, with the highest incidence being reported from Gezira State, Central Sudan. The present study was conducted at the Gezira Mycetoma Center and aimed to determine the cause of black-grain eumycetoma in the state and describe its epidemiology. Black-grain specimens were collected during the surgical operation and direct detection of the causative agent was performed using M. mycetomatis species-specific PCR and ITS PCR followed by sequencing. Black-grain was reported from 93.3% of all confirmed mycetoma cases (n = 111/119), with a prevalence in young males. Of the 91 samples subjected to direct PCR, 90.1% (n = 82) gave positive results. The predominant species (88.2%) was Madurella mycetomatis. One sample was identified as M. fahalii, one as M. tropicana, and one matched the phytopathogenic species Sphaerulina rhododendricola. The highest endemic zones were Southern Gezira (76.6%) and Northern Sinnar (23.4%). The study confirmed that direct molecular detection on grains provides rapid and specific diagnosis of agents of eumycetoma. |
format |
Article in Journal/Newspaper |
author |
Elhadi A Ahmed Bakri Y M Nour Adam D Abakar Samirah Hamid Ahmed A Mohamadani Mohamed Daffalla Mogahid Mahmoud Hisham N Altayb Marie Desnos-Ollivier Sybren de Hoog Sarah A Ahmed |
author_facet |
Elhadi A Ahmed Bakri Y M Nour Adam D Abakar Samirah Hamid Ahmed A Mohamadani Mohamed Daffalla Mogahid Mahmoud Hisham N Altayb Marie Desnos-Ollivier Sybren de Hoog Sarah A Ahmed |
author_sort |
Elhadi A Ahmed |
title |
The genus Madurella: Molecular identification and epidemiology in Sudan. |
title_short |
The genus Madurella: Molecular identification and epidemiology in Sudan. |
title_full |
The genus Madurella: Molecular identification and epidemiology in Sudan. |
title_fullStr |
The genus Madurella: Molecular identification and epidemiology in Sudan. |
title_full_unstemmed |
The genus Madurella: Molecular identification and epidemiology in Sudan. |
title_sort |
genus madurella: molecular identification and epidemiology in sudan. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2020 |
url |
https://doi.org/10.1371/journal.pntd.0008420 https://doaj.org/article/d27544a1884d44488c8ca1f72d31371f |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 14, Iss 7, p e0008420 (2020) |
op_relation |
https://doi.org/10.1371/journal.pntd.0008420 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0008420 https://doaj.org/article/d27544a1884d44488c8ca1f72d31371f |
op_doi |
https://doi.org/10.1371/journal.pntd.0008420 |
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PLOS Neglected Tropical Diseases |
container_volume |
14 |
container_issue |
7 |
container_start_page |
e0008420 |
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1766338158929117184 |