Dengue in Bali: Clinical characteristics and genetic diversity of circulating dengue viruses.
A high number of dengue cases are reported annually in Bali. Despite the endemicity, limited data on dengue is available for Bali localities. Molecular surveillance study was conducted to explore the clinical and virological characteristics of dengue patients in urban Denpasar and rural Gianyar area...
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ftdoajarticles:oai:doaj.org/article:d2554bef31344a62bdcebaac6b6d9179 2023-05-15T15:09:16+02:00 Dengue in Bali: Clinical characteristics and genetic diversity of circulating dengue viruses. Dewi Megawati Sri Masyeni Benediktus Yohan Asri Lestarini Rahma F Hayati Febrina Meutiawati Ketut Suryana Tangking Widarsa Dewa G Budiyasa Ngurah Budiyasa Khin S A Myint R Tedjo Sasmono 2017-05-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0005483 https://doaj.org/article/d2554bef31344a62bdcebaac6b6d9179 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC5456401?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0005483 https://doaj.org/article/d2554bef31344a62bdcebaac6b6d9179 PLoS Neglected Tropical Diseases, Vol 11, Iss 5, p e0005483 (2017) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2017 ftdoajarticles https://doi.org/10.1371/journal.pntd.0005483 2022-12-31T04:15:01Z A high number of dengue cases are reported annually in Bali. Despite the endemicity, limited data on dengue is available for Bali localities. Molecular surveillance study was conducted to explore the clinical and virological characteristics of dengue patients in urban Denpasar and rural Gianyar areas in Bali during the peak season in 2015. A total of 205 adult dengue-suspected patients were recruited in a prospective cross-sectional study. Demographic and clinical information were obtained, and dengue screening was performed using NS1 and IgM/IgG ELISAs. Viral RNA was subsequently extracted from patients' sera for serotyping using conventional RT-PCR and Simplexa Dengue real-time RT-PCR, followed by genotyping with sequencing method. We confirmed 161 patients as having dengue by NS1 and RT-PCR. Among 154 samples successfully serotyped, the DENV-3 was predominant, followed by DENV-1, DENV-2, and DENV-4. Serotype predominance was different between Denpasar and Gianyar. Genotyping results classify DENV-1 isolates into Genotype I and DENV-2 as Cosmopolitan Genotype. The classification grouped isolates into Genotype I and II for DENV-3 and DENV-4, respectively. Clinical parameters showed no relationship between infecting serotypes and severity. We observed the genetic diversity of circulating DENV isolates and their relatedness with historical data and importation to other countries. Our data highlights the role of this tourist destination as a potential source of dengue transmission in the region. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Bali ENVELOPE(-20.233,-20.233,64.067,64.067) PLOS Neglected Tropical Diseases 11 5 e0005483 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Dewi Megawati Sri Masyeni Benediktus Yohan Asri Lestarini Rahma F Hayati Febrina Meutiawati Ketut Suryana Tangking Widarsa Dewa G Budiyasa Ngurah Budiyasa Khin S A Myint R Tedjo Sasmono Dengue in Bali: Clinical characteristics and genetic diversity of circulating dengue viruses. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
A high number of dengue cases are reported annually in Bali. Despite the endemicity, limited data on dengue is available for Bali localities. Molecular surveillance study was conducted to explore the clinical and virological characteristics of dengue patients in urban Denpasar and rural Gianyar areas in Bali during the peak season in 2015. A total of 205 adult dengue-suspected patients were recruited in a prospective cross-sectional study. Demographic and clinical information were obtained, and dengue screening was performed using NS1 and IgM/IgG ELISAs. Viral RNA was subsequently extracted from patients' sera for serotyping using conventional RT-PCR and Simplexa Dengue real-time RT-PCR, followed by genotyping with sequencing method. We confirmed 161 patients as having dengue by NS1 and RT-PCR. Among 154 samples successfully serotyped, the DENV-3 was predominant, followed by DENV-1, DENV-2, and DENV-4. Serotype predominance was different between Denpasar and Gianyar. Genotyping results classify DENV-1 isolates into Genotype I and DENV-2 as Cosmopolitan Genotype. The classification grouped isolates into Genotype I and II for DENV-3 and DENV-4, respectively. Clinical parameters showed no relationship between infecting serotypes and severity. We observed the genetic diversity of circulating DENV isolates and their relatedness with historical data and importation to other countries. Our data highlights the role of this tourist destination as a potential source of dengue transmission in the region. |
format |
Article in Journal/Newspaper |
author |
Dewi Megawati Sri Masyeni Benediktus Yohan Asri Lestarini Rahma F Hayati Febrina Meutiawati Ketut Suryana Tangking Widarsa Dewa G Budiyasa Ngurah Budiyasa Khin S A Myint R Tedjo Sasmono |
author_facet |
Dewi Megawati Sri Masyeni Benediktus Yohan Asri Lestarini Rahma F Hayati Febrina Meutiawati Ketut Suryana Tangking Widarsa Dewa G Budiyasa Ngurah Budiyasa Khin S A Myint R Tedjo Sasmono |
author_sort |
Dewi Megawati |
title |
Dengue in Bali: Clinical characteristics and genetic diversity of circulating dengue viruses. |
title_short |
Dengue in Bali: Clinical characteristics and genetic diversity of circulating dengue viruses. |
title_full |
Dengue in Bali: Clinical characteristics and genetic diversity of circulating dengue viruses. |
title_fullStr |
Dengue in Bali: Clinical characteristics and genetic diversity of circulating dengue viruses. |
title_full_unstemmed |
Dengue in Bali: Clinical characteristics and genetic diversity of circulating dengue viruses. |
title_sort |
dengue in bali: clinical characteristics and genetic diversity of circulating dengue viruses. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2017 |
url |
https://doi.org/10.1371/journal.pntd.0005483 https://doaj.org/article/d2554bef31344a62bdcebaac6b6d9179 |
long_lat |
ENVELOPE(-20.233,-20.233,64.067,64.067) |
geographic |
Arctic Bali |
geographic_facet |
Arctic Bali |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 11, Iss 5, p e0005483 (2017) |
op_relation |
http://europepmc.org/articles/PMC5456401?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0005483 https://doaj.org/article/d2554bef31344a62bdcebaac6b6d9179 |
op_doi |
https://doi.org/10.1371/journal.pntd.0005483 |
container_title |
PLOS Neglected Tropical Diseases |
container_volume |
11 |
container_issue |
5 |
container_start_page |
e0005483 |
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1766340489799270400 |