Diversity, distribution and natural Leishmania infection of sand flies from communities along the Interoceanic Highway in the Southeastern Peruvian Amazon.
The Peruvian-Brazilian border is a highly endemic tegumentary leishmaniasis region in South America. The interoceanic highway is a commercial route that connects Peru and Brazil through Madre de Dios and has raised concerns about its impact on previously undisturbed areas. In order to assess leishma...
Published in: | PLOS Neglected Tropical Diseases |
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2021
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Online Access: | https://doi.org/10.1371/journal.pntd.0009000 https://doaj.org/article/d240e1f6afb84935a37a9bce88ebb7b5 |
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ftdoajarticles:oai:doaj.org/article:d240e1f6afb84935a37a9bce88ebb7b5 2023-05-15T15:17:05+02:00 Diversity, distribution and natural Leishmania infection of sand flies from communities along the Interoceanic Highway in the Southeastern Peruvian Amazon. Hugo O Valdivia Victor O Zorrilla Liz J Espada Jocelyn G Perez Hugo R Razuri Hubert Vera Roberto Fernandez Carlos Tong Bruno M Ghersi Gissella M Vasquez Roxanne G Burrus Andres G Lescano Joel M Montgomery 2021-02-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0009000 https://doaj.org/article/d240e1f6afb84935a37a9bce88ebb7b5 EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pntd.0009000 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0009000 https://doaj.org/article/d240e1f6afb84935a37a9bce88ebb7b5 PLoS Neglected Tropical Diseases, Vol 15, Iss 2, p e0009000 (2021) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2021 ftdoajarticles https://doi.org/10.1371/journal.pntd.0009000 2023-01-29T01:27:48Z The Peruvian-Brazilian border is a highly endemic tegumentary leishmaniasis region in South America. The interoceanic highway is a commercial route that connects Peru and Brazil through Madre de Dios and has raised concerns about its impact on previously undisturbed areas. In order to assess leishmaniasis transmission risk along this highway, we conducted a surveillance study of the sand fly populations in this area. Sand flies were collected between 2009 and 2010 along transects at 200 m, 600 m and 1000 m from six study sites located along the highway (Iberia, La Novia, Alto Libertad, El Carmen, Florida Baja, Mazuko and Mavila) and an undisturbed area (Malinowski). Collected specimens were identified based on morphology and non-engorged females of each species were pooled and screened by kinetoplast PCR to detect natural Leishmania infections. A total of 9,023 specimens were collected belonging to 54 different Lutzomyia species including the first report of Lu. gantieri in Peru. Four species accounted for 50% of all specimens (Lutzomyia carrerai carrerai, Lu. davisi, Lu. shawi and Lu. richardwardi). El Carmen, Alto Libertad, Florida Baja and Malinowski presented higher Shannon diversity indexes (H = 2.36, 2.30, 2.17 and 2.13, respectively) than the most human disturbed sites of Mazuko and La Novia (H = 1.53 and 1.06, respectively). PCR detected 10 positive pools belonging to Lu. carrerai carrerai, Lu. yuilli yuilli, Lu. hirsuta hirsuta, Lu. (Trichophoromyia) spp., and Lu. (Lutzomyia) spp. Positive pools from 1,000 m transects had higher infectivity rates than those from 600 m and 200 m transects (9/169 = 5.3% vs 0/79 = 0% and 1/127 = 0.8%, p = 0.018). El Carmen, accounted for eight out of ten positives whereas one positive was collected in Florida Baja and Mazuko each. Our study has shown differences in sand fly diversity, abundance and species composition across and within sites. Multiple clustered Lutzomyia pools with natural Leishmania infection suggest a complex, diverse and spotty role in leishmaniasis ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Baja Libertad ENVELOPE(-55.817,-55.817,-63.000,-63.000) PLOS Neglected Tropical Diseases 15 2 e0009000 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Hugo O Valdivia Victor O Zorrilla Liz J Espada Jocelyn G Perez Hugo R Razuri Hubert Vera Roberto Fernandez Carlos Tong Bruno M Ghersi Gissella M Vasquez Roxanne G Burrus Andres G Lescano Joel M Montgomery Diversity, distribution and natural Leishmania infection of sand flies from communities along the Interoceanic Highway in the Southeastern Peruvian Amazon. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
The Peruvian-Brazilian border is a highly endemic tegumentary leishmaniasis region in South America. The interoceanic highway is a commercial route that connects Peru and Brazil through Madre de Dios and has raised concerns about its impact on previously undisturbed areas. In order to assess leishmaniasis transmission risk along this highway, we conducted a surveillance study of the sand fly populations in this area. Sand flies were collected between 2009 and 2010 along transects at 200 m, 600 m and 1000 m from six study sites located along the highway (Iberia, La Novia, Alto Libertad, El Carmen, Florida Baja, Mazuko and Mavila) and an undisturbed area (Malinowski). Collected specimens were identified based on morphology and non-engorged females of each species were pooled and screened by kinetoplast PCR to detect natural Leishmania infections. A total of 9,023 specimens were collected belonging to 54 different Lutzomyia species including the first report of Lu. gantieri in Peru. Four species accounted for 50% of all specimens (Lutzomyia carrerai carrerai, Lu. davisi, Lu. shawi and Lu. richardwardi). El Carmen, Alto Libertad, Florida Baja and Malinowski presented higher Shannon diversity indexes (H = 2.36, 2.30, 2.17 and 2.13, respectively) than the most human disturbed sites of Mazuko and La Novia (H = 1.53 and 1.06, respectively). PCR detected 10 positive pools belonging to Lu. carrerai carrerai, Lu. yuilli yuilli, Lu. hirsuta hirsuta, Lu. (Trichophoromyia) spp., and Lu. (Lutzomyia) spp. Positive pools from 1,000 m transects had higher infectivity rates than those from 600 m and 200 m transects (9/169 = 5.3% vs 0/79 = 0% and 1/127 = 0.8%, p = 0.018). El Carmen, accounted for eight out of ten positives whereas one positive was collected in Florida Baja and Mazuko each. Our study has shown differences in sand fly diversity, abundance and species composition across and within sites. Multiple clustered Lutzomyia pools with natural Leishmania infection suggest a complex, diverse and spotty role in leishmaniasis ... |
format |
Article in Journal/Newspaper |
author |
Hugo O Valdivia Victor O Zorrilla Liz J Espada Jocelyn G Perez Hugo R Razuri Hubert Vera Roberto Fernandez Carlos Tong Bruno M Ghersi Gissella M Vasquez Roxanne G Burrus Andres G Lescano Joel M Montgomery |
author_facet |
Hugo O Valdivia Victor O Zorrilla Liz J Espada Jocelyn G Perez Hugo R Razuri Hubert Vera Roberto Fernandez Carlos Tong Bruno M Ghersi Gissella M Vasquez Roxanne G Burrus Andres G Lescano Joel M Montgomery |
author_sort |
Hugo O Valdivia |
title |
Diversity, distribution and natural Leishmania infection of sand flies from communities along the Interoceanic Highway in the Southeastern Peruvian Amazon. |
title_short |
Diversity, distribution and natural Leishmania infection of sand flies from communities along the Interoceanic Highway in the Southeastern Peruvian Amazon. |
title_full |
Diversity, distribution and natural Leishmania infection of sand flies from communities along the Interoceanic Highway in the Southeastern Peruvian Amazon. |
title_fullStr |
Diversity, distribution and natural Leishmania infection of sand flies from communities along the Interoceanic Highway in the Southeastern Peruvian Amazon. |
title_full_unstemmed |
Diversity, distribution and natural Leishmania infection of sand flies from communities along the Interoceanic Highway in the Southeastern Peruvian Amazon. |
title_sort |
diversity, distribution and natural leishmania infection of sand flies from communities along the interoceanic highway in the southeastern peruvian amazon. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2021 |
url |
https://doi.org/10.1371/journal.pntd.0009000 https://doaj.org/article/d240e1f6afb84935a37a9bce88ebb7b5 |
long_lat |
ENVELOPE(-55.817,-55.817,-63.000,-63.000) |
geographic |
Arctic Baja Libertad |
geographic_facet |
Arctic Baja Libertad |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 15, Iss 2, p e0009000 (2021) |
op_relation |
https://doi.org/10.1371/journal.pntd.0009000 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0009000 https://doaj.org/article/d240e1f6afb84935a37a9bce88ebb7b5 |
op_doi |
https://doi.org/10.1371/journal.pntd.0009000 |
container_title |
PLOS Neglected Tropical Diseases |
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15 |
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2 |
container_start_page |
e0009000 |
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1766347367761575936 |